ZEISS LSM Lightfield 4D On-demand Web Demo

Built for New Discoveries

Key Features of ZEISS LSM Lightfield 4D at a glance

Capture Spatial Signals & Fast Dynamics

Capture the fastest movements within whole organisms at up to 80 volumes per second with all spatiotemporal information intact. The unique one-snap-one-volume imaging ensures that crucial events are not missed or distorted.
Gently Observe Organisms Over Time

A complete Z-stack is acquired with a single illumination event, reducing light exposure and phototoxic effects to a minimum. Living samples can be imaged over long periods of time at high temporal density.
Speed Up Acquisition & Throughput

The unmatched speed with which Lightfield 4D captures multi-color volumes can be used to increase the productivity of experiments. Image and analyze more samples than ever before for statistically accurate and more meaningful data.
Combine Lightfield with Confocal Imaging

Take your experiments to the next level by pairing the flexibility of LSM (e.g., super-resolution imaging, spectral unmixing, and measurement of molecular dynamics) with the gentle and instant volume imaging of Lightfield 4D.

^{Image 1: Zebrafish heartbeat. Courtesy of Stone Elworthy and Emily Noël, School of Biosciences, University of Sheffield, UK. Data acquired at Wolfson Light Microscopy Facility in the School of Biosciences at the University of Sheffield.}

^{Image 2: Developing Zebrafish ear. Courtesy of Tanya Whitfield, Sarah Baxendale, School of Biosciences, University of Sheffield, UK. Data acquired at the Wolfson Light, Microscopy Facility, University of Sheffield.}

^{Image 3: Cleared Spheroid. Sample courtesy of InSphero AG, Schlieren, Switzerland.}

^{Image 4: Calcium signalling in the zebrafish brain. Courtesy of Anton Nikolaev, University of Sheffield, UK. Data acquired at Wolfson Light Microscopy Facility in the School of Biosciences at the University of Sheffield.}

Capture 3D Volumes in a Single Snapshot

Experience true instant volumetric imaging. ZEISS LSM 9 with Lightfield 4D captures 3D volumes from 37 angles in a single snapshot with no z-stack scanning needed. Researchers can record full datasets in seconds instead of minutes or hours, ideal for dynamic live samples like zebrafish or Drosophila embryos.

^{Image description - 3D information collection in Lightfield 4D: Different perspective views are generated on the camera sensor by the single lenslets depending on their position on the MLA.}

Ultra-Fast Imaging up to 80 volumes per second

Achieve ultra-fast 4D imaging at up to 80 volumes per second at cellular resolution. Perfect for capturing neural activity, heartbeat motion, or other rapid biological phenomena without delay or distortion.

Minimise Sample Stress, Maximise Insights

Lightfield 4D uses only one illumination per capture, dramatically reducing phototoxicity and photobleaching. This makes it a super-gentle technology for long-term developmental or live-cell imaging, preserving sample health even over hours of recording.

^{Sample courtesy of Andrew T Plygawko, School of Biosciences, University of Sheffield, UK. Data acquired at the Wolfson Light Microscopy Facility, University of Sheffield.}

One Microscope. Multiple Modalities.

Fully integrated into the powerful LSM 9 platforms, Lightfield 4D works seamlessly with existing LSM workflows, e.g., combining Lightfield 4D with super-resolution imaging of Airyscan 2, spectral unmixing using 32 channel GaAsP array detectors, or molecular dynamics measurement of FRAP/FRET/FCS.

^{Sample courtesy of Anton Nikolaev, University of Sheffield, UK. Data acquired at Wolfson Light Microscopy Facility in the School of Biosciences at the University of Sheffield.}

Download the ZEISS LSM Lightfield 4D Web Demo

Learn about the technology and its applications

In this web demo, learn about:

Introduction to imaging technology
Applications
Technical background
Data processing and analysis using ZEN and arivis software

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ZEISS LSM Lightfield 4D Web Demo

Built for New Discoveries

Capture Spatial Signals & Fast Dynamics

Gently Observe Organisms Over Time

Speed Up Acquisition & Throughput

Combine Lightfield with Confocal Imaging

Capture 3D Volumes in a Single Snapshot

Ultra-Fast Imaging up to 80 volumes per second

Minimise Sample Stress, Maximise Insights

One Microscope. Multiple Modalities.

Download the ZEISS LSM Lightfield 4D Web Demo

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