Imaging Workshop | Lighthouse Core Facility

Unlock the Full Potential of Your Confocal Microscope

Boost your skills

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Confocal Microscopy

Unlock the Full Potential of Your ZEISS Confocal Microscope

We warmly invite all scientists in the life sciences to participate in our upcoming workshop “Training Confocal Microscopy”. Whether you are a beginner or looking to deepen your expertise, this training is designed to boost your skills and confidence in using a ZEISS confocal microscope.

In this interactive workshop, we will cover both basic principles and advanced techniques. After completing the training, you will be able to operate your confocal system more confidently and even carry out complex multi-modal overnight experiments with ease.

What to Expect:

  • Fundamentals of Confocal Microscopy: Learn the core concepts and settings.
  • Advanced Techniques: Master multi-channel, time-lapse, and overnight imaging experiments.
  • Hands-on Sessions: Apply what you've learned immediately in practical exercises.

Special Feature: ZEISS Apotome 3 with Apotome Plus - Optical Sectioning in Widefield Fluorescence Microscopy

Discover how to achieve 3D widefield imaging with confocal-like quality using the ZEISS Apotome. With Joint Deconvolution, you can now experience optical sectioning at lateral resolutions down to 180 nm.

At a dedicated hands-on station, you’ll have the opportunity to:

  • Compare images before and after optical sectioning
  • Understand the underlying technology
  • Explore the possibilities Apotome 3 offers for your own research
  • Bring your own samples and test them live!

Language Options

We offer sessions in both German and English. You can choose the language option that best fits your preference when registering.

Don’t miss this opportunity to refine your confocal microscopy skills and experience cutting-edge technology first-hand. We look forward to welcoming you to an inspiring and practical workshop!

Agenda

  • Training konfokale Mikroskopie

    10  Uhr

    Willkommen

    10.15 Uhr

    Konfokale Mikroskopie: Grundprinzipien - der schnelle Weg zum ersten Bild

    11.15 Uhr

    Kaffeepause

    11.30 Uhr

    Bildaufnahme I: Aufnahmeparameter optimieren - Konfokale Detektion

    12.30 Uhr

    Mittagspause

    13.15 Uhr

    Bildaufnahme II: Mehrdimensionale Bildaufnahme: Z-Stapel, Zeitserien, Kacheln, Positionen, Fokusstrategien

    14.15 Uhr

    Kaffeepause

    14.30 Uhr

    Airyscanning: Grundprinzipien - Airyscan FAST - Multiplexing

    15.30 Uhr

    Kaffeepause

    15.45 Uhr

    Photomanipulation - Prozessierung - 3D Darstellung - Bildexport

    16.45 Uhr

    Ende

  • Training Confocal Microscopy

    10 AM

    Welcome 

    10.15 AM

    Confocal Microscopy: Basic Principles - The First Frames

    11.15 AM

    Coffee Break

    11.30 AM

    Image Acquisition I - Optimizing Imaging Parameters - Detectors

    12.30 PM

    Lunch

    1.15 PM

    Image Acquisition II - Multidimensional Acquisition: Z-Stacks, Time Series, Tiles, Focus Strategies

    2.15 PM

    Coffee Break

    2.30 PM

    Airyscanning: Basic principles - Airyscan FAST - Multiplexing

    3.30 PM

    Coffee Break

    3.45 PM

    Photo-Manipulation - Processing - 3D rendering - Image export

    4.45 PM

    End

  • ZEISS Axio Observer with Apotome 3 and Apotome PLUS

    Create brilliant optical sections of your fluorescent samples.​

    ZEISS LSM 900 with Airyscan 2

    Sensitive high-speed super-resolution imaging and molecular characterization.

Location

Universitätsklinikum Freiburg

IMITATE Breisacher Strasse 113 79106 Freiburg Germany

Registration

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