Laser Scanning Microscopy

Multicolor Imaging

From the Beginning to Channel Unmixing (2/3)

This course provides basic knowledge about multicolor imaging using the LSM 9 family with ZEN (blue edition) in respect to different configurations and hardware settings.

Course Details

Description

Learn how to evaluate your image quality regarding crosstalk and more important – learn how to overcome it. In addition, you will learn basic steps regarding linear unmixing in confocal channel mode.

Topics of the course

  • Emission and excitation crosstalk
  • Sequential vs. simultaneous image acquisition
  • Channel-based linear unmixing

Learning Level

Beginner

Prerequisites

Basic knowledge of light microscopy

Systems

LSM 9 family

Software

ZEN (blue edition)

Date

November 9, 2022
Registration until 2 weeks prior to the course

Duration

1 day (4 hours)
Course takes place in the European timezone (CET/CEST)

Location

Virtual classroom
(Required: reliable internet connection; computer speakers, headphones or a reliable telephone connection; Microsoft Teams and/or TeamViewer)

Language

English

Course Fee

Per person: 450 € (plus VAT)

Participants

Max. 3

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