Light Microscopy

Polarized Light Microscopy

Examination of Birefringent Objects

Polarized light microscopy is used to detect birefringence within microscopically small structures (e.g. mineral grains, textile fibers, polymers, parts of plants/ animals etc.). This makes a discrimination and characterization of objects possible and allows for conclusions about the molecular or atomic built- up of such birefringent objects.

Course Details


Moreover, with this sensitive method the impact of external parameters (temperature, pressure etc.) on transparent, birefringent objects/ raw material becomes possible to investigate. You will learn, how to optimally make use of your polarization microscope. You will learn, how to interpret the images properly.

Topics of the course

  • Practical handling (alignment and workflow) of the polarized light microscope
  • The application of polarized light microscopy as a contrasting method in transmitted and reflected light
  • Birefringence measurement (optical retardation) in transmitted light
  • Explanation of the theoretical background of phenomena, instrument and measurement tools without using complex mathematical context

Learning Level



Basic knowledge of light microscopy (e.g. Microscopy from the very Beginning) and recommended basic polarization knowledge


Stemi 508
Axiolab 5 Conoscopy
Axioscope 5 Pol
Axio Imager.A2m Pol




February 21 to 23, 2023
Registration until 2 weeks prior to the course


3 days (3 x 6 hours)
Course takes place in the European timezone (CET/CEST)


Virtual classroom
(Required: reliable internet connection; computer speakers, headphones or a reliable telephone connection; Microsoft Teams and/or TeamViewer)



Course Fee

Per person: 1418 € (plus VAT)


Max. 4

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