Light Microscopy

Superresolution Microscopy

@ Elyra 7

Superresolution microscopy enables insights into structures beyond the classical resolution limit. Since the introduction of Elyra 7, structured illumination microscopy (SIM) has been brought to a new level. This course will provide the skills to acquire Lattice SIM, PALM, and dSTORM data from specimens and will enable you to perform the appropriate processing.

Course Details


Groundbreaking light efficiency empowers gentle superresolution imaging with incredibly high speed: to examine the fastest processes in living samples – in large fields of view, in 3D, over long time periods, and with multiple colors. Elyra 7 combines Lattice SIM with single molecule localization microscopy (SMLM) for techniques such as PALM, dSTORM and PAINT.

Topics of the course

Lattice SIM

  • Principle, acquisition and filtering
  • Versatility: SIM Mode, Leap Mode, Apotome Mode, Burst Mode
  • Camera alignment, channel alignment
  • PSF measurement
  • Processing

Single-molecule localization microscopy 

  • Basics and applications
  • Comparison: SMLM is more than PALM
  • Multichannel imaging
  • Grouping and working with fiducials
  • Activation strategies
  • 3D PALM

Learning Level



Profound knowledge of laser scanning microscopy


Elyra 7


ZEN SR black


January 31 to February 3, 2023
Registration until 2 weeks prior to the course


4 days


Training and Support Center Jena



Course Fee

Per person: 3028 € (plus VAT)


Max. 3

Book Course

Please fill in the required fields. Make sure to put your invoice address in the address boxes. Afterwards you will receive a confirmation email.

Form is loading...

Click here  to read our statement on data protection.

Contact Us

For additional information please send a request to