Light Microscopy

Superresolution Microscopy

@ Elyra 7

Superresolution microscopy enables insights into structures beyond the classical resolution limit. Since the introduction of Elyra 7, structured illumination microscopy (SIM) has been brought to a new level. This course will provide the skills to acquire Lattice SIM, PALM, and dSTORM data from specimens and will enable you to perform the appropriate processing.

Course Details

Description

Groundbreaking light efficiency empowers gentle superresolution imaging with incredibly high speed: to examine the fastest processes in living samples – in large fields of view, in 3D, over long time periods, and with multiple colors. Elyra 7 combines Lattice SIM with single molecule localization microscopy (SMLM) for techniques such as PALM, dSTORM and PAINT.

Topics of the course

Lattice SIM

  • Principle, acquisition and filtering
  • Versatility: SIM Mode, Leap Mode, Apotome Mode, Burst Mode
  • Camera alignment, channel alignment
  • PSF measurement
  • Processing

Single-molecule localization microscopy 

  • Basics and applications
  • Comparison: SMLM is more than PALM
  • Multichannel imaging
  • Grouping and working with fiducials
  • Activation strategies
  • 3D PALM

Learning Level

Expert

Prerequisites

Profound knowledge of laser scanning microscopy

Systems

Elyra 7

Software

ZEN SR black

Date

January 31 to February 3, 2023
Registration until 2 weeks prior to the course

Duration

4 days

Location

Training and Support Center Jena

Language

English

Course Fee

Per person: 2 880 € (plus VAT)

Participants

Max. 3

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