ZEISS Primostar 3 iLED Your Fluorescence Microscope for Sputum Examination
ZEISS Primostar 3 iLED is your microscope to visualize small structures down to 0.2 – 5 μm. So you can even observe objects such as the rod-shaped Mycobacterium tuberculosis. The gold standard for sputum smear microscopy is Ziehl-Neelsen staining and brightfield light microscopy. According to WHO1, LED fluorescence microscopy is even more sensitive and less time-consuming, making it a real alternative to the conventional standard.
With Primostar 3 iLED you:
Fluorescence or Brightfield
The Choice is Yours
Easily change between fluorescence and brightfield. Auramine-rhodamine fluorescently labeled mycobacteria light up greenish yellow in front of a dark background. Your images will show excellent contrast.
With Fluorescence up to Four Times Faster
Using fluorescence and the 40× objective lens of your Primostar 3 iLED allows to visualize structures smaller than 0.5 μm such as Mycobacterium tuberculosis. With the large field of view you identify such details up to four times faster than with brightfield microscopy.2
Stop TB Partnership
According to the WHO1, 10 million people fall ill with tuberculosis (TB) every year. 1.5 million even die. Those numbers make TB the world top infectious disease. It is caused by the bacteria Mycobacterium tuberculosis, which was detected by Robert Koch in 1882. As a member of the Stop TB Partnership ZEISS is following Koch's footsteps in the battle against TB.
How to Stain Auramine-Rhodamine
In this video tutorial Dr Harald Hoffmann, MD and head of the WHO-certified supranational reference laboratory IML in Gauting, Germany, demonstrates how to stain smear preparations for diagnosis of tuberculosis with fluorescence microscopy.
*The protocol and microscope recommendation shown in the video is based on the experience of the WHO reference laboratory in Gauting.