Microscopy Courses, Training and Education

The main aim is to understand the basic concept of Axio Scan.Z1 and learn how to operate the system for brightfield and fluorescence scanning including maintenance and tips and tricks. The knowledge transfer is realized mainly by hands-on.

The course’ objectives are to expand your knowledge on our powerful Tiles & Position module and understand what applications require which focus strategy. Generally, the course aims to make you feel more comfortable when working with those modules and improve your knowledge on how to do what and where to find what you need.

After a short review of the basics of ZEN core, we train advanced functions in theory and experiment.

You will learn about the theoretical foundations of ZEN 2 core and get insights into the practical application. Working in small groups what has been learned can be immediately put into practice.

After an introduction to the ZEN programming language and the functions of the macro editor in ZEN, application examples of automatic image acquisition and image analysis are presented and programmed by the user.

Advanced fluorescence techniques, commonly known as the F-techniques, measure the kinetics and the interactions of biomolecules with high sensitivity and spatiotemporal resolution. This course provides detailed theoretical and practical knowledge about principles and applications of the most commonly used F-techniques: Fluorescence Recovery After Photo-bleaching (FRAP) & Förster Resonance Energy Transfer (FRET) on the LSM 9 family.

Fluorescence Correlation Spectroscopy (FCS) is a highly sensitive method that detects movement and binding of fluorescence-labeled particles on the single molecule level. This course will familiarize you with the theoretical background of FCS and train you on how to perform and evaluate measurements in solutions.

Laser Scanning Microscopy is a standard method in biomedical research. Excellent knowledge of its theory and application is a prerequisite for experimental success. This course provides detailed knowledge about point scanning confocal microscopes of the LSM 9 family with ZEN Blue in respect to its assembly, features and application in biomedical sciences. 

This course provides basic knowledge about multicolor imaging using the LSM 9 family with ZEN Blue in respect to different configurations and hardware settings. Learn how to evaluate your image quality regarding crosstalk and more important – learn how to overcome it. In addition, you will learn basic steps regarding linear unmixing in confocal channel mode.

Multiphoton microscopy is a preferred method for non-invasive and deep tissue imaging of living or fixed samples. The course provides theoretical knowledge of this imaging technique and practical hands-on exercise in ZEN (blue edition).

Do you want to image large fields of view and whole sample volumes in shortest possible time? And do you want to image with superb image quality at the same time? The LSM 9 family with Airyscan 2 now gives you more options to fit imaging speeds and resolution to your experimental needs. This course provides detailed knowledge about the Airyscan 2 technology with the new Multiplex mode. 

This course provides detailed knowledge about spectral imaging using the LSM 9 family with ZEN Blue in respect to different configurations and hardware settings. Learn workflows of typical applications, e.g. how to separate overlapping dyes and auto fluorescence or detect multiple dyes simultaneously and gently using the spectral detector in online fingerprinting mode.