Imagine you had access to an imaging system that could deliver optical sections of large samples, with virtually no phototoxicity or bleaching and with high temporal resolution.
That is exactly what Lightsheet Z.1 from ZEISS does. The unique Multiview light sheet fluorescence microscope allows you to record the development of large, living samples and gently image them to deliver exceptionally high information content.
It is also fast: Lightsheet Z.1 is the tool you need to get optical sections at unprecedented speed. Acquire images of your whole sample volume at sub-cellular resolution – in a fraction of the time it takes using other techniques.
"We are very satisfied with Lightsheet Z.1 microscope. We are developing sample preparation protocols for diverse plant species and materials and successfully use them for long-term imaging of plant development under favourable physiological conditions. We can warmly recommend this instrument to your customers."
Prof. Dr. Jozef Šamaj
Palacký University Olomouc
Centre of the Region Haná for Biotechnological and Agricultural Research
June 2, 2015
Mouse hippocampus, optically cleared in LUMOS agent. Data processing and 3D rendering was done with arivis Vision4D™
Mouse brain, optically cleared in LUMOS agent. Stitching and 3D volume rendering was done with arivis Vision4D™
Tissue clearing allows you to image deep into large biological samples such as tissue sections, brains, embryos, organs, spheroids or biopsies. You can use its enhanced optical penetration depth to capture fluorescent signals of whole organs. This makes clearing a promising technique when, for example, investigating neuronal networks in mouse brains.
Lightsheet Z.1 combines the advantages of clearing with light sheet fluorescence microscopy. It lets you image large cleared specimens with exceptional light efficiency, speed and next to no photo damage. Now you can acquire multiple tiles of Z-stacks with several thousand high quality images. A typical imaging speed of 10-40 frames per second reduces your imaging time from hours to minutes.
Use Lightsheet Z.1 with Clr Plan-Apochromat 20x/1.0 Corr nd=1.38 to perform experiments with tissue cleared by Scale medium (Hama et al, Nat Neurosci. 2011), which has a refractive index of n=1.38. If your aqueous clearing medium has a refractive index of n=1.45, you can choose either Clr Plan-Neofluar 20x/1.0 Corr nd=1.45 (optimized for FocusClear™ by CelExplorer Labs, http://www.celexplorer.com, the embedding medium for CLARITY (Chung et al, Nature 2013) or Lightsheet Z.1 detection optics 5x/0.16 to investigate your sample.
The sample holder with its easy-to-access interface gives you the flexibility to adapt the sample mounting to your specific needs. Different adapters will either support your sample from below or let you mount the sample hanging from above: you are always free to choose the perfect viewing angle.
Because Lightsheet Z.1 is the gentlest way to observe the development of living specimens, it opens up a new world of experiments for your research.
These are just some of the light sheet microscopy applications that are now available to you:
Scroll through the playlist to observe the work of scientists who are using Lightsheet Z.1
Light sheet fluorescence microscopy or LSFM splits fluorescence excitation and detection into two separate light paths, with the axis of illumination perpendicular to the detection axis. That means you illuminate a single thin section of your sample at one time, generating an inherent optical section by exciting only fluorescence from the in-focus plane. No pinhole or image processing is required for light sheet microscopy.
Light from the in-focus plane is collected on the pixels of a camera, rather than pixel by pixel as, for example, in confocal or other laser scanning microscopy approaches. Parallelization of the image collection on a camera-based detector lets you collect images faster and with less excitation light than you would with many other microscope techniques.
In summary, LSFM combines the optical sectioning effect with parallel image acquisition from the complete focal plane. This makes 3D imaging extremely fast and very light efficient.
Light Sheet Fluorescence Microscopy for Multiview Imaging of Large Specimens
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Refractive Index on Demand
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Integration of Daylight Illumination into Time Lapse Experiments
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Protocols and Guidelines for ZEISS Lightsheet Z.1
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Multiview Imaging of Large Specimens
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Stitching with arivis Vision4D ZEISS Edition
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