Digital Slide Scanner

ZEISS Axioscan 7 offers reliably reproducible image quality, no matter whether you repeat your imaging task after a day, a week, a month or even on a different machine.

Introducing Transfer of Intensity Equation (TIE), the new contrast method for contrast generation in transparent samples. Now you can record the interaction of a narrow cone of light with your sample’s structures in three images: one in focus and two out of focus above and below the focal plane. From these three images, the phase information for the central plane is automatically extracted. Continuous acquisition in the z dimension, combined with flash illumination and GPU-based fast image processing, enables very fast delivery of the final contrast images. Present this as either phase contrast or DIC-like relief contrast: it’s your choice.

TIE contrast is an excellent tool that supports your experiments when working with sensitive fluorescent dyes:

• Detect transparent tissues with little to no contrast in regular brightfield mode.
• Speed up the subsequent fluorescence imaging process with very fast flash-based focusing.
• Protect your sensitive dyes from bleaching during focusing by using the lowest light doses.
• Bring your fluorescent labels into context easily by applying additional contrast information.

The UltiMapper I/O PD-L1 kit from Ultivue looks at whether a tumor is ‘hot‘ or ’cold’. It also addresses whether the tumor will respond to immune checkpoint inhibition because of a high immune filtrate (hot in contrast to tumors with low immune infiltrates ( ‘cold tumors’ or non-T-cell-inflamed cancers). It does this by exploring multiple cell phenotypes such as cytotoxic immune cells (CD8), immunosuppressive macrophages (Markers CD68, PD-L1) or immune evading tumor cells (Markers CK, PD-L1).

Please note. The following images represent research content. ZEISS explicitly excludes the possibility of making a diagnosis or recommending treatment for possibly affected patients on the basis of the information generated with the Axioscan 7 spatial biology slide scanner.

Use different forms of polarization illumination to highlight different features. Plane polarized light (PPL) shows the overall crystal color, habit and pleochroism. Crossed polarized light (XPL) at multiple orientations lets you assess the extinction angle and birefringence. Circular polarization allows maximum birefringence to be observed regardless of grain orientation. All channels are aligned using powerful computational algorithms during acquisition, making the resulting data perfect for subsequent segmentation and analysis.