Super-resolution image of a large field of view of HeLa cells. Courtesy of A. Politi, J. Jakobi and P. Lenart, MPI for Biophysical Chemistry, Göttingen, Germany.
Microscopy Applications for Live Sciences

3D Cell Imaging

With Fluorescence Microscopy

Whether you study cell compartmentalization, protein trafficking, cytoskeleton, cell division, cell death and apoptosis, stem cells and differentiation or many other topics in cell biology, fluorescence microscopy provides critical information for your research. When selecting a microscopy technology, you must carefully consider:

  • How many fluorophores are required?
  • How much resolution do you need?
  • How sensitive are your samples?
  • Do your experiments require high-content imaging?

ZEISS has the most powerful portfolio of fluorescence imaging systems to support your cell biology and cancer research.

  • Cortical neurons stained for DNA, microtubules and microtubule-associated proteins
    Cortical neurons stained for DNA, microtubules and microtubule-associated proteins

    Cortical neurons stained for DNA, microtubules and microtubule-associated proteins imaged with ZEISS Apotome 3. Courtesy of L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany.
     

    Cortical neurons stained for DNA, microtubules and microtubule-associated proteins imaged with ZEISS Apotome 3. Courtesy of L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany.
     

    Structured Illumination

    Economical 3D Fluorescence Imaging

    Widefield microscopes, such as the ZEISS Axio Observer inverted microscope or the ZEISS Axio Imager 2 upright microscope, can be used with the ZEISS Apotome 3 structured illumination system to block out of focus light and increase the resolution of your 3D fluorescence data.

    Apotome 3 is robust, easy-to-use and economical compared to confocal and other advanced fluorescence systems making it an ideal choice for single labs working with 3D fluorescent, fixed cells and tissues.

  • Cos7 cells imaged with LSM Plus, including the ZEISS NIR detector in channel mode.
    Cos7 cells imaged with LSM Plus, including the ZEISS NIR detector in channel mode.

    Cos7 cells imaged with LSM Plus, including the ZEISS NIR detector in channel mode. Sample courtesy of U. Ziegler and J. Doehner, University of Zurich, ZMB, Switzerland.
     

    Cos7 cells imaged with LSM Plus, including the ZEISS NIR detector in channel mode. Sample courtesy of U. Ziegler and J. Doehner, University of Zurich, ZMB, Switzerland.
     

    Confocal Microscopy

    Flexibility in Fluorophore Choice with Options for Super-resolution Imaging and Higher Throughput

    The revolutionary design of the ZEISS LSM 9 family of confocal microscopes offer high sensitivity for crisp imaging even with delicate samples.

    Spectral flexibility for use of multiple fluorophores with overlapping excitation and emission spectra is possible with capabilities into the NIR as well as LSM Plus for improved resolution of spectral data.

    Airyscan 2 can be added for super-resolution capabilities and now, with jDCV, the resolution of the system can be pushed ~90 nm laterally.

    Multiplex mode is also available for increased speeds and higher throughput.

  • Lattice SIM² image of Cos-7 cells labeled with phalloidin Alexa 488
    Lattice SIM² image of Cos-7 cells labeled with phalloidin Alexa 488

    Lattice SIM² image of Cos-7 cells labeled with phalloidin Alexa 488. Maximum intensity projection of Z stack.
     

    Lattice SIM² image of Cos-7 cells labeled with phalloidin Alexa 488. Maximum intensity projection of Z stack.
     

    Super-resolution Light Microscopy

    With Resolution Down to 60 nm

    ZEISS Elyra 7 with Lattice SIM² takes you far beyond the diffraction limit of conventional microscopy for superresolution; you can now double the conventional SIM resolution and discriminate the finest sub-organelle structures, even those no more than 60 nm apart, using standard fluorophores. Image up to four conventional fluorophores for your sub-cellular investigations.

  • Eightfold symmetry of the nuclear pore complex in the frog kidney cell line, A6. Gp210 was labeled with Alexa Fluor 647.
    Eightfold symmetry of the nuclear pore complex in the frog kidney cell line, A6. Gp210 was labeled with Alexa Fluor 647.


    Eightfold symmetry of the nuclear pore complex in the frog kidney cell line, A6. Gp210 was labeled with Alexa Fluor 647. Widefield image (top row, left), SMLM image (top row, right) and zoomed in region (bottom row).


    Eightfold symmetry of the nuclear pore complex in the frog kidney cell line, A6. Gp210 was labeled with Alexa Fluor 647. Widefield image (top row, left), SMLM image (top row, right) and zoomed in region (bottom row).

    3D Imaging

    At Molecular Resolution

    ZEISS Elyra 7 offers single molecule localization microscopy (SMLM) for techniques such as PALM, dSTORM and PAINT to achieve lateral resolution of 20 – 30 nm, the highest super-resolution level for light microscopy. In addition, Elyra 7 provides you with 3D SMLM mode based on PRILM technology for 3D data from a whole cell with consistent molecular precision.

  • Multiplex imaging of non-small cell lung cancer (NSCLC) tissue stained with UltiMapper I/O PD-L1 kit.
    Multiplex imaging of non-small cell lung cancer (NSCLC) tissue stained with UltiMapper I/O PD-L1 kit.

    Multiplex imaging of non-small cell lung cancer (NSCLC) tissue stained with UltiMapper I/O PD-L1 kit. Nuclear counterstain (blue), CD8 (green), CD68 (orange), PD-L1 (red), panCytoKeratin(magenta) acquired with ZEISS Axioscan 7. Sample courtesy of Ultivue, Inc. Cambridge, Massachusetts, USA

    Multiplex imaging of non-small cell lung cancer (NSCLC) tissue stained with UltiMapper I/O PD-L1 kit. Nuclear counterstain (blue), CD8 (green), CD68 (orange), PD-L1 (red), panCytoKeratin(magenta) acquired with ZEISS Axioscan 7. Sample courtesy of Ultivue, Inc. Cambridge, Massachusetts, USA

    Automated Microscopy

    For High-Content Fluorescence Cell Imaging

    If your experiment demands higher throughput, automation is necessary to collect the data you need. ZEISS Axioscan 7 offers automated, high-quality imaging of up to 100 slides in a single run for high-content imaging and screening. Set up your experiment and let it run overnight or over the weekend. Axioscan 7 software is built to flawlessly process large amounts of raw data – in the range of several terabytes.

  • Plunge-frozen adenocarcinoma cells grown on sapphire discs.
    Plunge-frozen adenocarcinoma cells grown on sapphire discs.


    Plunge-frozen adenocarcinoma cells grown on sapphire discs. All regions of interest are shown in context in ZEN Connect


    Plunge-frozen adenocarcinoma cells grown on sapphire discs. All regions of interest are shown in context in ZEN Connect

    Combine Your Fluorescence Microscopy Data

    With Ultrastructural Information

    The combination of fluorescence microscopy with electron microscopy provides a powerful data set of both function and structure. Native morphology can only be observed when you freeze your sample instead of using chemical fixatives. ZEISS field emission SEMs and FIB-SEMs support cryo workflows and can image your delicate samples with outstanding performances at low voltages. ZEISS has developed a seamless Correlative Cryo Workflow which connects fluorescence widefield, laser scanning confocal and focused ion beam scanning electron microscopy in a seamless and easy-to-use procedure.

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    * The images shown on this page represent research content. ZEISS explicitly excludes the possibility of making a diagnosis or recommending treatment for possibly affected patients on the basis of the information generated with an Axioscan 7 slide scanner.