Fluorescence imaging is used in a wide application range from immunohistochemical labeling of fixed samples up to tagging dynamic molecules in living cells using fluorescence proteins. Samples can vary strongly from thick organoids to thinnest cell extensions like lamellipodia and filopodia. Furthermore, specimen can be mounted on a large variety of sample carriers from standard slides to dedicated 96 wells.
To cover this huge variety of samples and applications a flexible imaging tool is needed that guarantees reliable results. ZEISS Celldiscoverer 7 with LSM 900 Airyscan 2 offers the necessary options for fast and gentle fluorescence imaging in widefield or confocal mode – mixed mode imaging is also possible. Sample carriers are automatically detected. The system calibrates automatically the optical system to the used sample bottom to guarantee highest image quality and reliability. The system is completely controlled by the ZEN software for daily easy-to-use routine microscopy and screening applications.
In this workshop we will present the technical features and possible applications of Celldiscoverer 7 followed by detailed hands-on sessions.