ZEN Correlative Array Tomography
Software

ZEN Correlative Array Tomography

3D Light and Electron Microscopy for Serial Sections

With Array Tomography, you use serial sections to reconstruct your sample volume. You cut resin-embedded tissue samples with an ultramicrotome into consecutive sections and image them. After automated acquisition in the light microscope, you transfer the sample to your electron microscope where you find the same software tools. Image hundreds of sections across length scales automatically and combine them into one single correlative volume dataset.

  • Large-scale 3D correlative microscopy
  • Wizard-guided workflows
  • Precise reconstruction of sample volumes
Assembly of a correlative 3D dataset

Perform Large-Scale 3D Correlative Microscopy

Correlative imaging of a large number of serial sections has never been easier: Simply outline one of the sections in your ribbon, and ZEN Correlative Array Tomography automatically detects and marks all other sections. You then sketch the region of interest (ROI) in any section. The software suggests an optimal tiling setup and automatically transfers the ROI to all other sections.

Serial sections (green frame) after the automatic section detection

Enjoy Wizard-Guided Workflows

ZEN Correlative Array Tomography offers the same working environment on the light and electron microscope, adapted to their respective requirements. Let software wizards guide you step-by-step through the process of acquiring hundreds of serial sections on the light microscope. Then transfer the sample to your electron microscope. ZEN Correlative Array Tomography provides you with all the information you need to seamlessly start imaging the same regions at nanometer resolution.

3D reconstruction of serial sections from root nodules with the distribution of plasmodesmata. Courtesy of D. Sherrier, J. Caplan, E. Kmiec and S. Modla, University of Delaware, USA.

Reconstruct Your Correlative 3D Data Set

Use ZEN Correlative Array Tomography to stitch, align and reconstruct images from your light and electron microscope into 3D stacks. Then combine corresponding LM and EM datasets into one correlative 3D volume. You get an improved z-resolution based on the section thickness and not on optical constraints. Investigate the finest details in your data from light and electron microscopy – all in one software.

Symbiotic Relationship of Plants and Bacteria in Root Nodules

  • 3D reconstruction of serial sections from root nodules with the distribution of plasmodesmata.

    Courtesy of D. Sherrier, J. Caplan, E. Kmiec and S. Modla, University of Delaware, USA.

Downloads

    • ZEN Correlative Array Tomography

      3D Correlative Light and Electron Microscopy for Serial Sections

      Pages: 13
      File size: 1 MB
    • ZEN Correlative Array Tomography

      Sample Preparation for Correlative Array Tomography

      Pages: 8
      File size: 1 MB

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