How-to Video

How to Set up Koehler Illumination

On Your Upright Transmitted Light Microscope

8 April 2020 · 3 min watch
  • Teaching
  • Widefield Light Microscopy

Abstract

How to Set up Koehler Illumination: On Your Upright Transmitted Light Microscope

You want to image your samples with optimized contrast and resolution?

Then you need to apply Köhler illumination, a process that provides homogeneous illumination without stray light. It still is the only method to obtain reproducible quantitative data in light microscopy.

How-to Video

  • How to Set up Koehler Illumination

    On Your Upright Transmitted Light Microscope


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