How-to Video
How to Set up Koehler Illumination
On Your Upright Transmitted Light Microscope
8 April 2020
· 3 min watch
Abstract
How to Set up Koehler Illumination: On Your Upright Transmitted Light Microscope
You want to image your samples with optimized contrast and resolution?
Then you need to apply Köhler illumination, a process that provides homogeneous illumination without stray light. It still is the only method to obtain reproducible quantitative data in light microscopy.
![Using Zebrafish to Spark Student Interest in Science]({"xsmall":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/undergraduate-students-get-an-edge-with-advanced-microscopy/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg/_jcr_content/renditions/original.image_file.100.56.0,113,1280,833.file/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg","small":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/undergraduate-students-get-an-edge-with-advanced-microscopy/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg/_jcr_content/renditions/original.image_file.360.203.0,113,1280,833.file/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg","medium":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/undergraduate-students-get-an-edge-with-advanced-microscopy/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg/_jcr_content/renditions/original.image_file.768.432.0,113,1280,833.file/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg","large":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/undergraduate-students-get-an-edge-with-advanced-microscopy/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg/_jcr_content/renditions/original.image_file.1024.576.0,113,1280,833.file/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg","xlarge":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/undergraduate-students-get-an-edge-with-advanced-microscopy/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg/_jcr_content/renditions/original.image_file.1280.720.0,113,1280,833.file/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg","xxlarge":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/undergraduate-students-get-an-edge-with-advanced-microscopy/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg/_jcr_content/renditions/original.image_file.1280.720.0,113,1280,833.file/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg","max":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/undergraduate-students-get-an-edge-with-advanced-microscopy/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg/_jcr_content/renditions/original.image_file.1280.720.0,113,1280,833.file/student-at-sigma-field-emission-scanning-electron-microscope-lyon.jpg"} "Using Zebrafish to Spark Student Interest in Science")
![Winner in Education: Vitamin C crystals, acquired with ZEISS Axioscope]({"xsmall":"https://www.zeiss.com/content/dam/z/rms/reference-master/campaigns/sector-independent/fy-21-22/2022-zeiss-microscopy-image-contest/robert_berdan_vitamin_c_crystals_education_prev.jpg/_jcr_content/renditions/original.image_file.100.56.file/robert_berdan_vitamin_c_crystals_education_prev.jpg","small":"https://www.zeiss.com/content/dam/z/rms/reference-master/campaigns/sector-independent/fy-21-22/2022-zeiss-microscopy-image-contest/robert_berdan_vitamin_c_crystals_education_prev.jpg/_jcr_content/renditions/original.image_file.360.203.file/robert_berdan_vitamin_c_crystals_education_prev.jpg","medium":"https://www.zeiss.com/content/dam/z/rms/reference-master/campaigns/sector-independent/fy-21-22/2022-zeiss-microscopy-image-contest/robert_berdan_vitamin_c_crystals_education_prev.jpg/_jcr_content/renditions/original.image_file.768.432.file/robert_berdan_vitamin_c_crystals_education_prev.jpg","large":"https://www.zeiss.com/content/dam/z/rms/reference-master/campaigns/sector-independent/fy-21-22/2022-zeiss-microscopy-image-contest/robert_berdan_vitamin_c_crystals_education_prev.jpg/_jcr_content/renditions/original.image_file.1024.576.file/robert_berdan_vitamin_c_crystals_education_prev.jpg","xlarge":"https://www.zeiss.com/content/dam/z/rms/reference-master/campaigns/sector-independent/fy-21-22/2022-zeiss-microscopy-image-contest/robert_berdan_vitamin_c_crystals_education_prev.jpg/_jcr_content/renditions/original.image_file.1280.720.file/robert_berdan_vitamin_c_crystals_education_prev.jpg","xxlarge":"https://www.zeiss.com/content/dam/z/rms/reference-master/campaigns/sector-independent/fy-21-22/2022-zeiss-microscopy-image-contest/robert_berdan_vitamin_c_crystals_education_prev.jpg/_jcr_content/renditions/original.image_file.1440.810.file/robert_berdan_vitamin_c_crystals_education_prev.jpg","max":"https://www.zeiss.com/content/dam/z/rms/reference-master/campaigns/sector-independent/fy-21-22/2022-zeiss-microscopy-image-contest/robert_berdan_vitamin_c_crystals_education_prev.jpg/_jcr_content/renditions/original./robert_berdan_vitamin_c_crystals_education_prev.jpg"} "Winner in Education: Vitamin C crystals, acquired with ZEISS Axioscope")
![Using Zebrafish to Spark Student Interest in Science]({"xsmall":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/using-zebrafish-to-spark-student-interest-in-science/student-at-stereo-microscope-zebrafish-larvae.png/_jcr_content/renditions/original.image_file.100.56.0,3,1080,611.file/student-at-stereo-microscope-zebrafish-larvae.png","small":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/using-zebrafish-to-spark-student-interest-in-science/student-at-stereo-microscope-zebrafish-larvae.png/_jcr_content/renditions/original.image_file.360.203.0,3,1080,611.file/student-at-stereo-microscope-zebrafish-larvae.png","medium":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/using-zebrafish-to-spark-student-interest-in-science/student-at-stereo-microscope-zebrafish-larvae.png/_jcr_content/renditions/original.image_file.768.432.0,3,1080,611.file/student-at-stereo-microscope-zebrafish-larvae.png","large":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/using-zebrafish-to-spark-student-interest-in-science/student-at-stereo-microscope-zebrafish-larvae.png/_jcr_content/renditions/original.image_file.1024.576.0,3,1080,611.file/student-at-stereo-microscope-zebrafish-larvae.png","xlarge":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/using-zebrafish-to-spark-student-interest-in-science/student-at-stereo-microscope-zebrafish-larvae.png/_jcr_content/renditions/original.image_file.1080.608.0,3,1080,611.file/student-at-stereo-microscope-zebrafish-larvae.png","xxlarge":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/using-zebrafish-to-spark-student-interest-in-science/student-at-stereo-microscope-zebrafish-larvae.png/_jcr_content/renditions/original.image_file.1080.608.0,3,1080,611.file/student-at-stereo-microscope-zebrafish-larvae.png","max":"https://www.zeiss.com/content/dam/z/rms/reference-master/resource-center/insights-hub/user-story/using-zebrafish-to-spark-student-interest-in-science/student-at-stereo-microscope-zebrafish-larvae.png/_jcr_content/renditions/original.image_file.1080.608.0,3,1080,611.file/student-at-stereo-microscope-zebrafish-larvae.png"} "Using Zebrafish to Spark Student Interest in Science")
![ZEISS Axioscope 5]({"xsmall":"https://www.zeiss.com/content/dam/z/rms/reference-master/products/light/widefield/axioscope-5/axioscope-5-fl_axiocam-208-color.jpg/_jcr_content/renditions/original.image_file.100.100.392,0,1472,1080.file/axioscope-5-fl_axiocam-208-color.jpg","small":"https://www.zeiss.com/content/dam/z/rms/reference-master/products/light/widefield/axioscope-5/axioscope-5-fl_axiocam-208-color.jpg/_jcr_content/renditions/original.image_file.360.360.392,0,1472,1080.file/axioscope-5-fl_axiocam-208-color.jpg","medium":"https://www.zeiss.com/content/dam/z/rms/reference-master/products/light/widefield/axioscope-5/axioscope-5-fl_axiocam-208-color.jpg/_jcr_content/renditions/original.image_file.768.768.392,0,1472,1080.file/axioscope-5-fl_axiocam-208-color.jpg","large":"https://www.zeiss.com/content/dam/z/rms/reference-master/products/light/widefield/axioscope-5/axioscope-5-fl_axiocam-208-color.jpg/_jcr_content/renditions/original.image_file.1024.1024.392,0,1472,1080.file/axioscope-5-fl_axiocam-208-color.jpg","xlarge":"https://www.zeiss.com/content/dam/z/rms/reference-master/products/light/widefield/axioscope-5/axioscope-5-fl_axiocam-208-color.jpg/_jcr_content/renditions/original.image_file.1080.1080.392,0,1472,1080.file/axioscope-5-fl_axiocam-208-color.jpg","xxlarge":"https://www.zeiss.com/content/dam/z/rms/reference-master/products/light/widefield/axioscope-5/axioscope-5-fl_axiocam-208-color.jpg/_jcr_content/renditions/original.image_file.1080.1080.392,0,1472,1080.file/axioscope-5-fl_axiocam-208-color.jpg","max":"https://www.zeiss.com/content/dam/z/rms/reference-master/products/light/widefield/axioscope-5/axioscope-5-fl_axiocam-208-color.jpg/_jcr_content/renditions/original.image_file.1080.1080.392,0,1472,1080.file/axioscope-5-fl_axiocam-208-color.jpg"} "ZEISS Axioscope 5")