ZEISS LSM 880 with Airyscan

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LSM 880 with Airyscan

Your New Standard for Fast and Gentle Confocal Imaging

Dr. Ralf Engelmann, product manager, introducing LSM 880 with Airyscan.

To get ahead in your research you may want to image the smallest structures, catch the faintest signals or track the fastest processes – or do all of that at once. When it comes to getting accurate data from live cells or other weakly-labeled samples, there is no such thing as too much sensitivity, resolution or speed. Each photon of emission light is precious.

With Airyscan you have the unrivaled combination of fast superresolution and sensitive confocal image acquisition at hand. Use multicolor samples with any label and get image quality like you’ve never seen before. Decide on this novel detector design and get a 4-8× improvement in signal-to-noise ratio (SNR) while simultaneously achieving a 1.7x increase in resolution. You will also be harnessing this unique combination of greater SNR and resolution at increased acquisition speeds of 27fps (at 480 x 480 pixels).

Highlights

A New World of Confocal Performance

  • Gain sensitivity, resolution and speed for all your experiments. Image with negligible phototoxicity or bleaching - without changing your workflow, sample labels or system operation.
  • Airyscan’s unique Fast mode speeds up your imaging by a factor of four. This will propel you into the traditional domain of resonant scanning confocals without sacrificing sensitivity or resolution.
  • Airyscan delivers superresolution with high sensitivity at 140 nm laterally and 400 nm axially. This transcends the deconvolution approach by preserving precious emission light normally rejected at a closed pinhole.

Resonance Scanner using standard confocal


 

LSM 880 Airyscan Fast

Videos: Cardiomyocyte Cells with tubulin-EMTB to measure microtubule buckling.
Top video: Resonance Scanner using standard confocal; Acquisition speed: 80fps. 
Bottom video: LSM 880 Airyscan Fast; Acquisitionspeed: 96 fps. 
Images and Samples courtesy of Ben Prosser, University of Pennsylvania, USA “Detyrosinated microtubules buckle and bear load in contracting cardiomyocytes”, Science April 2016.

HeLa cells
HeLa cells

Enhanced resolution with Airyscan. HeLa cells, Courtesy of S. Traikov, BIOTEC, TU Dresden, Germany

Enhanced resolution with Airyscan. HeLa cells, Courtesy of S. Traikov, BIOTEC, TU Dresden, Germany

Sample: U2OS Cells with Lifeactin; Max projection of 6 plane z-stack, 50 volumes over 17.5 minutes.


The patented Airyscan detector design allows gentle live cell imaging with superresolution. You collect more photons than with the closed pinhole of a traditional LSM and deconvolution. 

Application Examples
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Increase Your Productivity

  • Combine the Fast mode for Airyscan with Z-stacks and tiling to image even large sample volumes with high image quality.
  • Collect all your fluorescent signals in one go. Parallel acquisition lets you investigate multiple labels in minimum time, equipped with the highest number of detectors of any confocal.
  • Improve your image quality with the unique combination of parallel spectral acquisition and high speed GPU deconvolution.
  • Make the most of large fields of view and the highest speed of any linear scanning confocal – ZEISS LSM 880 with Airyscan delivers up to 27 fps at 480x480 pixels in Fast mode.

Drosophila embryo, depth coded maximum intensity projection. Airyscan in Fast mode. Courtesy of B. Erdi, Max F. Perutz Laboratories, University of Vienna, Austria

 

Confocal Drosophila Brain NDD Airyscan.
Confocal Drosophila Brain NDD Airyscan.

Combine the resolution and sensitivity advantage of Airyscan with multiphoton excitation for superior imaging deep in your specimens. Confocal Drosophila Brain Zoom NDD Airyscan.

Confocal Drosophila Brain NDD Airyscan. 

 

Use high frame rates in large fields of view with lowest light exposure for live imaging of whole organisms.


Combine the resolution and sensitivity advantage of Airyscan with multiphoton excitation for superior imaging deep in your specimens. 

 

  • Combine the resolution and sensitivity advantage of Airyscan with multiphoton excitation for superior imaging deep in your specimens. 
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Application Examples
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Choose the Most Flexible Confocal

  • Tailor Airyscan to your research needs with a choice of Superresolution, Sensitivity mode or the new Fast mode.
  • Remove autofluorescence and simultaneously separate highly overlapping fluorophores in a single scan. This will minimize phototoxicity in the sample.
  • Combine confocal imaging with single molecule techniques to gain mobility/concentration/oligomeric state information (FCS/FCCS/RICS/PCH).
  • Select Airyscan’s Fast mode to perform functional multiphoton imaging deep inside your sample.
Choanoflagellate rosette colony.

Choanoflagellate rosette colony. Blue: nuclei stained with Hoechst; magenta: tubulin of the flagella and cell body; green: actin microvilli collars stained with phalloidin. Courtesy of H. Aaron, UC Berkeley, USA

Choanoflagellate rosette colony.

Choanoflagellate rosette colony. Blue: nuclei stained with Hoechst; magenta: tubulin of the flagella and cell body; green: actin microvilli collars stained with phalloidin. Courtesy of H. Aaron, UC Berkeley, USA

 

Mitosis in HeLa-Kyoto cell line, imaged with ZEISS LSM 880 with Airyscan. Video showing Histone 2B (H2B, red, mCherry) and microtubule end binding protein 3 (EB3, green, EGFP) during mitosis.

Combine your data from multiple complementary imaging modalities – e.g., widefield, confocal and three different superresolution techniques. You can even correlate data with electron microcopy.


Gentle superresolution imaging allows long-term live cell imaging without artefacts caused by influencing the sample’s viability and biological function. 

Application Examples
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Proof of performance – see the difference

Slide between a standard confocal and the Airyscan Fast mode

Standard Confocal - 15 FPS; Pixel Dwell 320ns; Effective Laser Dosage 2.2x Airyscan Fast Mode - 30 FPS; Pixel Dwell 580ns; Color Coded Time Projection

Improve your highly dynamic Ca2+ imaging with the Fast mode for Airyscan

You capture more signal at high speeds while keeping light exposure to a minimum

Airyscan in Fast mode for Calcium Spark Imaging. With Airyscan in Fast superresolution mode you get more structural information at high speeds. The donut shape of the Golgi in plant cells is nicely resolved.

The technology behind it

The Airyscan Principle

Airyscan solves this conundrum between resolution and light efficiency by imaging the Airy disk onto a concentrically-arranged hexagonal detector array.

Airyscan principle

Your Powerful Combination

Add Airyscan with the Fast module to image with up to 27 frames per second at 480 × 480 pixels with highest image quality.

Powerful combination

 

Parallel Acquisition of Multiple Channels

Gain new insights into biological structures and processes by acquiring the entire fluorescent spectra of all your labels at once.

Parallel acquisition

Application Samples

  • Images
    Drosophila embryo. Color coded maximum intensity projection of the central nervous system.

    Drosophila embryo. Color coded maximum intensity projection of the central nervous system. Courtesy of J. Sellin, AG Hoch, LIMES Institute, Bonn, Germany.

    Drosophila embryo. Color coded maximum intensity projection of the central nervous system.

    Drosophila embryo. Color coded maximum intensity projection of the central nervous system. Courtesy of J. Sellin, AG Hoch, LIMES Institute, Bonn, Germany

    IMR90 human diploid lung fibroblasts.

    IMR90 human diploid lung fibroblasts. Sample: Courtesy of J. Karlseder, Molecular and Cell Biology Laboratory; J. Fitzpatrick, Waitt Advanced Biophotonics Core, Salk Institute for Biological Studies, La Jolla, USA.

    IMR90 human diploid lung fibroblasts.

    IMR90 human diploid lung fibroblasts. DNA has been stained with DAPI, the telomeric G strand (leading strand) in green with a Peptide Nucleic Acid probe and Alexa 488 and the telomeric C strand (lagging strand) in red with a Peptide Nucleic Acid probe and Alexa 546. Prior to their harvest the cells have been treated with siRNAs targeting RTEL1. RTEL1 is a helicase that is essential for telomere replication, and lack of the protein leads to stalled forks at telomeres and telomere breakage. This can be seen by individual telomeres that appear as more than one dot, as highlighted in the images. Airyscan resolves multiple telomere dots, thereby allowing an accurate quantification of telomere replication problems. Sample: Courtesy of J. Karlseder, Molecular and Cell Biology Laboratory; J. Fitzpatrick, Waitt Advanced Biophotonics Core, Salk Institute for Biological Studies, La Jolla, USA.

    Human RPE cells

    Human RPE cells, ZO1 (tight junction marker) in blue, photoreceptor outer segments stained with FITC in green, EEA1 (endosomal marker) in red. Courtesy of S. Almewadar, CRTD, TU Dresden, Germany

    Human RPE cells

    Human RPE cells, ZO1 (tight junction marker) in blue, photoreceptor outer segments stained with FITC in green, EEA1 (endosomal marker) in red. Courtesy of S. Almewadar, CRTD, TU Dresden, Germany

  • Videos

    HeLa cells stained for Actin (green), Adapter Protein AP-3 (magenta) and Septin A (red). Courtesy of S. Traikov, BIOTEC, TU Dresden, Germany

    Scroll through the Playlist to discover Airyscan Technology.

    Fixed tumor cells, tubulin labelled with Alexa 555, Airyscan SR mode. Sample courtesy of P. O'Toole and P. Pryor, University of York, UK. 

    Oligodendrocyte, CNPase-antibody staining. Courtesy of C. Dornblut, Leibniz Institute for Age Research (FLI), Jena, Germany

    Drosophila embryo, maximum intensity projection. Microtubules labelled with GFP. Left: z-stack with 55 slices. Imaged for 203 min at 3 min interval. Courtesy of B. Erdi, Max F. Perutz Laboratories, University of Vienna, Austria

    Drosophila embryo, maximum intensity projection. Microtubules labelled with GFP. Imaged at higher magnification. Z-stack with 117 slices, imaged for 75 min at 3 min interval. Courtesy of B. Erdi, Max F. Perutz Laboratories, University of Vienna, Austria

Downloads

ZEISS LSM 880 with Airyscan

Your New Standard for Fast and Gentle Confocal Imaging

Pages: 36
Filesize: 11,605 kB

White Paper: Airyscanning - Short version

A Novel Approach to Confocal Imaging

Pages: 5
Filesize: 1,709 kB

Beampath - ZEISS LSM 880 with Airyscan

Revolutionize Your Confocal Imaging

Pages: 1
Filesize: 607 kB

Application Note: Spectral Imaging: a Powerful Tool for Confocal Multicolor Imaging in Living Plant Cells

Pages: 7
Filesize: 1,138 kB

Application Note: Cryo-Confocal Imaging with Airyscan

Improving Resolution and Signal-to-Noise in Cryo-Fluorescence Microscopy

Pages: 8
Filesize: 1,483 kB

Technology Note: The Airyscan Detector from ZEISS

Confocal Imaging with Improved Signal-to-Noise Ratio and Superresolution

Pages: 19
Filesize: 3,958 kB

Technology Note: ZEISS LSM 880 with Airyscan

Introducing the Fast Acquisition Mode

Pages: 7
Filesize: 1,071 kB

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