Apotome.2  - Optical Sectioning Using Structured Illumination


Optical sectioning using structured illumination

Create optical sections of your fluorescent samples – free of scattered light. With structured illumination, you know that only the focal plane appears in your image: Apotome.2 recognizes the magnification and moves the appropriate grid into the beampath. The system then calculates your optical section from three images with different grid positions without time lag. It’s a totally reliable way to prevent scattered out-of-focus light, even in your thicker specimens. Operate your system just as easy as always. You get images with high contrast in the best possible resolution – simply brilliant optical sections.



Perfect Images – with All Magnifications

Because your applications need different objectives, you need a system that gives you the best resolution for each one. Apotome.2 automatically uses the right grid for your objective, selecting from three grids with different frequencies. With a defined optical section thickness in the region of a Rayleigh unit, you profit from simply brilliant images.

Optimum Results – Free Choice of Light Source and Dyes

From conventional HBO illumination to adjustment free metal-halide lamp HXP 120 C to Colibri.2, the LED illumination source that is gentle on your samples: with Apotome.2 you use exactly the light you need. Apotome.2 also gives you the choice of fluorophores. Whether you work with DAPI, FITC, Rhodamin, Cy5 or with vital dyes such as GFP or mRFP, it’s your decision, not the technology’s. Just change the filter and your system automatically moves the grid to the correct position. From DAPI to Cy5, you get perfect optical sections for multichannel imaging.



Brilliant Images – Even with Thick Specimens

Your optical section thickness is close to one Rayleigh unit, a value that stands for high axial resolution with a good signal-to-noise ratio. Apotome.2 increases the resolution in Z direction compared to conventional fluorescence microscopy: you obtain brilliant optical sections that allow 3D-rendering , even from thick specimens.




Optical sectioning (right) comparison with widefield microscopy (left).
Mouse embryo, tissue section, green: GFP, red: Cy3.
Objective: Plan APOCHROMAT 40 x/1.3 Oil.
Courtesy of N. Büttner, T. Vogel, Centre for Anatomy, University of Göttingen, Germany.


Rat hippocampus, triple fluorescence, maximum projection of 3D image stack.
Objective: Plan-APOCHROMAT 63x/1.4.
Courtesy of E. Fuchs & S. Bauch, DPZ Göttingen, Germany.


Drosophila embryo, green: HRP, red: glia marker, 100µm Z-stack.
Courtesy of C. Klämbt, Institute for Neurobiology, University of Münster, Germany.


ZEISS Apotome.2

Optical Sections in Fluorescence Imaging

Pages: 16
Filesize: 6,688 kB

Product Information

For the iBooks version: (interactive iBook; 119 MB)

Either download the file to your computer and perform a manual sync with iTunes, or download the file directly onto your iOS device via WiFi and the Safari Browser.


Quick Reference Guide (Multilanguage)

ApoTome.2 Imaging system

Operating Manual (English)

ApoTome.2 Imaging system