Optical Sections in Fluorescence Imaging
Because your applications need different objectives, you need a system that gives you the best resolution for each one. Apotome.2 automatically uses the right grid for your objective, selecting from three grids with different frequencies. With a defined optical section thickness in the region of a Rayleigh unit, you profit from simply brilliant images.
From conventional HBO illumination to adjustment free metal-halide lamp HXP 120 C to Colibri.2, the LED illumination source that is gentle on your samples: with Apotome.2 you use exactly the light you need. Apotome.2 also gives you the choice of fluorophores. Whether you work with DAPI, FITC, Rhodamin, Cy5 or with vital dyes such as GFP or mRFP, it’s your decision, not the technology’s. Just change the filter and your system automatically moves the grid to the correct position. From DAPI to Cy5, you get perfect optical sections for multichannel imaging.
Your optical section thickness is close to one Rayleigh unit, a value that stands for high axial resolution with a good signal-to-noise ratio. Apotome.2 increases the resolution in Z direction compared to conventional fluorescence microscopy: you obtain brilliant optical sections that allow 3D-rendering , even from thick specimens.
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