PALM MicroBeam

PALM MicroBeam is designed to give you advanced laser microdissection capabilities. Highlights of this patented, future-oriented and upgradable technology from Carl Zeiss include:

• Suitability for laser microdissection and analysis for DNA, RNA and protein isolation  — whether from archive material or live cells
• A system that completes the step from laser-microdissection to integrated imaging workstation
• Applicable to cryosections, FFPE (formalin fixed and paraffin embedded) tissue
• Performing LCM even from standard glass slides, e.g. archived material, with ZEISS-patented sample capture technology
• Extendibility — add digital cameras from the AxioCam camera range for fluorescence, bright field, multi-channel fluorescence and extended focus
• Ability to integrate additional components into MicroBeam and create simple workflows, whether for individual experiments or automation with flexible collectors
• Calibration routines that allow you to easily adjust MicroBeam for laser parameters appropriate to your chosen specimen.

When you are investigating DNA, you need to select your source material carefully. Even sensitive methods of analysis can amplify unwanted material — and the slightest contamination can alter your results. If you are testing individual cells, it only takes one unwanted cell to result in a contamination error of 100%.

PALM MicroBeam solves this problem by allowing you to isolate high-purity biomolecules from heterogeneous tissue safely, quickly and reproducibly. For example, you can separate tumor tissue with a high level of purity and carry out exact, error-free and reproducible mutation analyses. You also successfully investigate archived specimens from pathology or forensics with PALM MicroBeam — and in contrast to other laser microdissection techniques you are able to use your normal glass slides.

Because analyses of gene expression patterns rely on exactly- separated analytical material, unwanted cells can alter your results and conceal the signals of the relevant cells. PALM MicroBeam prevents this happening by allowing you to define cells and tissue regions precisely, ensuring your results are exact and reproducible.