ZEISS Axiocam 820 mono
Cameras

ZEISS Axiocam 820 mono

Your Sensitive 20 Megapixel Microscope Camera for Demanding, Large Field of View, Fluorescence Applications

The Axiocam 820 mono 20 megapixel camera is equipped with a large 17.5 mm diagonal back-illuminated CMOS sensor having a peak quantum efficiency of 86%. In combination with its low readout noise, this camera is the perfect choice for imaging faint fluorescence signals in living or fixed samples. Its square sensor efficiently utilizes the optical field of view of the microscope for capturing more details. The dual USB 3.0 interface enables the highest acquisition speeds without sacrificing the robustness of a standardized interface. The high performance and unmatched flexibility of the Axiocam 820 mono camera makes it the perfect choice for the most challenging samples in life science research.

Murine lung tissue. Sample courtesy of A. Feuchtinger, Helmholtz Zentrum München, Germany

ZEISS Axiocam 820 mono is Recommended for

  • The most demanding fluorescence applications in life sciences
  • Imaging dim fluorescent signals with a good signal-to-noise ratio
  • Fast tile scanning applications
  • Dynamic events in live cell imaging
  • Flexible setups with varying applications
Quantum efficiency
Quantum efficiency

Quantum efficiency

Quantum efficiency

Highlights of Axiocam 820 mono

  • 20 megapixel back-thinned square CMOS sensor with 17.5 mm diagonal
  • 28 full-resolution images per second
  • 30 frames per second in live image mode
  • Small 2.74 micron pixels for resolving the finest details at all magnifications
  • High 86% QE by back illuminated sensor architecture
  • Low readout noise of 1.3 e- by high-quality noise inhibition technology for low light imaging
  • Wide sensitivity spectrum from 350 nm to 1000 nm
  • Fast read-out with global shutter architecture for distortion-free images
  • Reproducible image quality due to active thermal stabilization of the sensor
  • Robust, very fast and easy-to-use dual USB 3.0 connection
  • Hardware triggering

Application Examples

Mouse pancreas tissue

Mouse pancreas tissue

Mouse pancreas tissue
Sample courtesy: A. Feuchtinger, Helmholtz Zentrum München, Germany

Stained for DNA (blue), insulin (green) and glucagon (red) on pancreas islet.

Mouse pancreas tissue

Stained for DNA (blue), insulin (green) and glucagon (red) on pancreas islet.

Sample courtesy: A. Feuchtinger, Helmholtz Zentrum München, Germany

Murine lung tissue with tumor metastasis

Murine lung tissue with tumor metastasis

Murine lung tissue with tumor metastasis
Sample courtesy of H. Ishikawa-Ankerhold, Walter-Brendel-Zentrum für Experimentelle Medizin München, Germany.

Fixed with 4% PFA and stained for: tumor cells (RFP), macrophages (siglecH-GFP), T-cells (Ly6-G647) and DNA (DAPI).

Murine lung tissue with tumor metastasis

Fixed with 4% PFA and stained for: tumor cells (RFP), macrophages (siglecH-GFP), T-cells (Ly6-G647) and DNA (DAPI).

Sample courtesy of H. Ishikawa-Ankerhold, Walter-Brendel-Zentrum für Experimentelle Medizin München, Germany

Downloads

    • ZEISS Axiocam 820 mono

      Your Sensitive 20 Megapixel Microscope Camera for Demanding, Large Field of View, Fluorescence Applications

      Pages: 4
      File size: 2 MB
    • ZEISS Axiocam Family

      Your Guide to Microscope Camera Technology from ZEISS

      Pages: 44
      File size: 15 MB

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