Incubators typically restrict the access to your sample, making formerly simple tasks like positioning, focusing and generation of an overview image more complicated. AI Sample Finder performs these tasks with the push of a button. Application of immersion media to your water immersion objectives is performed in less than a second without changing focus, stage position, or disturbing the incubation chamber for independent, automated imaging. Environmental parameters are automatically recorded so that you can be confident that the observed dynamics are not caused by fluctuating temperature or atmosphere. Additionally, ZEISS Axio Observer live is equipped with advanced software tools for streamlined analyses of large, multivalued timelapse experiments. ​

Multi-position, extended time lapse experiment with automated immersion:
When working with living samples, you might not know where your event of interest may occur. To capture the uptake of nanoparticles by macrophages, many locations from a multi-well plate are acquired as well as multiple z-planes over several hours at 37°C using re-immersion. The region shown above is a subset of the much larger dataset that was captured using automated imaging and shows the uptake of nanoparticles inside the cells (top row). The surface of the cells were also imaged to verify that the nanoparticles are inside the cells and not simple sitting on the cell surface (bottom row).

^{Sample courtesy: F. Páez Larios and C. Eggeling, Institute for Applied Optics and Biophysics, Friedrich-Schiller-Universität Jena, Germany}

Live cell experiments over extended periods with automated immersion:
HEK KO PEX5 cells expressing eGFP with a photocaged peroxisomal targeting signal type 1 were reconstituted with the peroxisomal import receptor PEX5. A light induced conformational change of the photocage leads to exposure of the peroxisomal targeting signal. If the WT PEX5 is expressed, accumulation of the eGFP signal in the dotted peroxisomes can be monitored (top row). In case of the mutated PEX5 (bottom row), even after 18 hours, no peroxisomal import could be detected.

^{Sample courtesy of K. Reglinski, Institute for Applied Optics and Biophysics, Friedrich-Schiller-Universität Jena, Germany}