ZEISS Axio Observer section

Name: ZEISS Axio Observer section
Brand: ZEISS

High-resolution 3D visualization and evaluation

3D imaging at high resolution is important to understand the spatial context of your biological structures of interest. ZEISS Axio Observer section provides streamlined workflows for cost-effective 3D imaging from data collection to evaluation. Image thick tissues without out-of-focus light blur and improve your segmentation results. Create stunning 3D renderings for visualizing details you haven’t seen before with your widefield microscope.

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3D cell culture models (organoids, spheroids)
Small model organisms (yeast, C. elegans, zebrafish)
Cell monolayers
Tissue sections

Detect rare events like mitosis with guided acquisition. Improve image quality with deconvolution. Visualize your results in 3D.

A Streamlined process from acquisition to 3D visualization

Equip yourself with tools for guided and automated image acquisition to break through previous experimental barriers. Utilize software-based optical sectioning as the most light- and cost-efficient method for high-resolution 3D imaging. Or include ZEISS Apotome 3 structured illumination for increased optical sectioning power and gain sharp images even of thicker samples. Process your images during acquisition using one of several functions like deconvolution, deblurring or denoising.

Bundle components

Microscope	Axio Observer 7 (inverted widefield microscope stand) Scanning stage 130 × 100 Motorized condenser NA 0.55 AI Sample Finder Apotome 3
Light source / camera	Viluma 7 Filter sets 38, 43, 50, 64, 96, 115 Axiocam 820 mono
Objectives	EC Plan-Neofluar 5× / 0.16 Plan-Apochromat 10× / 0.45 Plan-Apochromat 20× / 0.8 Plan-Apochromat 63× / 1.4 Oil
Workstation	Z8 Workstation with 128 GB RAM and nVidia Quadro RTX A4000 16 GB DP

Motorized Acquisition	Acquire images and control motorized components in multi-dimensional experiments.
2D Toolkit	Create flexible automatic measurement programs for the analysis of your 2D images.

Looking for an upright microscope configuration?

This widefield bundle for high-resolution 3D visualization and evaluation can also be configured based on the upright microscope ZEISS Axio Imager 2.

Axio Imager section

Example applications

Courtesy of F. A. Ditengou, University of Freiburg, Germany.
Autofluorescence of a Lotus Japonicus root infected with symbiotic bacteria stained with mcherry.
Courtesy of H. Reuter, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany.
Transgenic zebrafish larvae at 4 days post fertilization staining for: Glial fibrillary acidic protein, acetylated Tubulin, GFP and DNA. Embedded in 1.2% low melt agarose.
Sample courtesy: P. Hemmerich, Leibniz Institute on Aging – Fritz Lipmann Institute e. V. (FLI), Germany
Maximum intensity projection of PML nuclear bodies of U-2 OS cells transiently transfected with mRFP-SUMO-2 (magenta) and pEGFP-PML-I (green). Note that overexpression of PML and SUMO-2 leads to an increased size of the nuclear bodies. Image sizes: 2×2 µm.

Downloads

ZEISS Microscope Bundle for Optical Sectioning

Creating crisp sections of thick samples with ease

328 KB

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Visit the ZEISS Download Center for available translations and further manuals.

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ZEISS Axio Observer section

A Streamlined process from acquisition to 3D visualization

Bundle components