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Tissue Clearing and 3D Light Sheet Imaging of Neural Circuits in the Intact and Injured Spinal Cord

JAN 19, 2022 · 41 watch
  • Life Sciences
  • Light Sheet Microscopy
Author

Dr. Dylan McCreedy

Assistant Professor
Texas A&M University (USA)

Abstract

Tissue Clearing and 3D Light Sheet Imaging of Neural Circuits in the Intact and Injured Spinal Cord

Tissue clearing and 3D light sheet imaging have enabled detailed interrogation of neural circuits in the brain, however, corresponding studies in the spinal cord have been more limited. We have optimized aqueous- and organic solvent-based tissue clearing protocols, which has enabled high-resolution imaging of the full spinal cord using light sheet microscopy. Molecularly-defined neuronal subsets can then be readily visualized in the cleared spinal cord using multiple fluorescent labeling and reporter systems. We are currently developing high-throughput image analysis methods to generate a 3D map of neural circuits in the intact spinal cord and to characterize damage that occurs following spinal cord injury. The knowledge gained from these studies will advance our understanding of motor and sensory systems in the spinal cord and provide critical insight into repair strategies to restore function lost due to injury.

Key Learnings:

  • Comprehensive overview of how to prepare cleared mouse spinal cords for fluorescence microscopy
  • Overview of fluorescent markers and reporters used to visualize different neuronal cell subtypes within whole mouse spinal cords
  • Analysis of spinal cord injury using light sheet microscopy

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