![超微細構造の高分解能イメージング – 電子顕微鏡による生物試料の観察 超微細構造の高分解能イメージング – 電子顕微鏡による生物試料の観察]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.100.100.file/fe-sem_dendritic-cell_3view_2.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.360.360.file/fe-sem_dendritic-cell_3view_2.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.768.768.file/fe-sem_dendritic-cell_3view_2.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/fe-sem_dendritic-cell_3view_2.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/fe-sem_dendritic-cell_3view_2.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/fe-sem_dendritic-cell_3view_2.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/fe-sem_dendritic-cell_3view_2.jpg/_jcr_content/renditions/original./fe-sem_dendritic-cell_3view_2.jpg"})
超微細構造の高分解能イメージング
電子顕微鏡による生物試料の観察
電子顕微鏡のみが提供できる最高レベルの分解能が必要とされるのは、たとえば、試料の奥深くにある小さな構造の3Dイメージング、試料本来の超微細構造を観察するような場合です。ZEISSの電子顕微鏡を使用することで、細胞研究や癌研究、微生物学やウイルス学、免疫学、神経科学、発生学、発生生物学、植物科学において、生物試料の構造を高分解能でイメージングすることが可能となります。
![マウス脳、オスミウム・チオカルボヒドラジド染色、ZEISS GeminiSEM 300でイメージング。試料ご提供:C. Genoud, FMI, Basel, Switzerland マウス脳、オスミウム・チオカルボヒドラジド染色、ZEISS GeminiSEM 300でイメージング。試料ご提供:C. Genoud, FMI, Basel, Switzerland]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1920,1082.file/geminisem-braindetail.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1920,1082.file/geminisem-braindetail.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1920,1082.file/geminisem-braindetail.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1920,1082.file/geminisem-braindetail.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1280.720.0,0,1920,1082.file/geminisem-braindetail.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1440.810.0,0,1920,1082.file/geminisem-braindetail.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1920.1080.0,0,1920,1082.file/geminisem-braindetail.jpg"})
![マウス脳、オスミウム・チオカルボヒドラジド染色、ZEISS GeminiSEM 300でイメージング。試料ご提供:C. Genoud, FMI, Basel, Switzerland マウス脳、オスミウム・チオカルボヒドラジド染色、ZEISS GeminiSEM 300でイメージング。試料ご提供:C. Genoud, FMI, Basel, Switzerland]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.100.56.file/geminisem-braindetail.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.360.203.file/geminisem-braindetail.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.768.432.file/geminisem-braindetail.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1024.576.file/geminisem-braindetail.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1280.720.file/geminisem-braindetail.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original.image_file.1440.810.file/geminisem-braindetail.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/geminisem-braindetail.jpg/_jcr_content/renditions/original./geminisem-braindetail.jpg"})
マウス脳、オスミウム・チオカルボヒドラジド染色、ZEISS GeminiSEM 300でイメージング。
試料ご提供:C. Genoud, FMI, Basel, Switzerland
マウス脳、オスミウム・チオカルボヒドラジド染色、ZEISS GeminiSEM 300でイメージング。
試料ご提供:C. Genoud, FMI, Basel, Switzerland
超微細構造の高分解能イメージング
ZEISS GeminiSEMシリーズやZEISS Sigmaシリーズなどの電界放出型SEM(FE-SEM)を使用することで、サブナノレベルの高分解能で高コントラストのイメージングを行えます。FE-SEMでは、樹脂包埋試料の超薄切片内の細胞内構造、細胞小器官、ウイルスをTEMレベルで正確に視覚化できます。
事前セクショニング不要の高分解能3Dイメージング
高分解能3Dイメージングにより、コネクトミクスでの単細胞や組織の観察、および発生生物学での生物全体の観察において、詳細な構造情報を得ることができます。
ZEISS FE-SEM用の3Viewを使用したブロックフェイスイメージングでは、試料ブロックをチェンバー内で直接スライスし、その度にイメージングを行います。この方法によって、最小Z軸スライス厚15 nmで数百万立法マイクロメートルまでの大きな3D像を観察することができます。
また、ZEISS Crossbeamの集束イオンビームを使用した加工とFIB-SEMトモグラフィー解析により、最小Z軸分解能3 nmの超解像3Dイメージングが可能です。
![マウス脳切片、最高取得速度1.22ギガピクセル/秒。 マウス脳切片、最高取得速度1.22ギガピクセル/秒。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.100.67.0,0,1917,1280.file/multisem-mouse-brain.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.360.240.0,0,1917,1280.file/multisem-mouse-brain.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.768.512.0,0,1917,1280.file/multisem-mouse-brain.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1024.683.0,0,1917,1280.file/multisem-mouse-brain.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1280.853.0,0,1917,1280.file/multisem-mouse-brain.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1440.960.0,0,1917,1280.file/multisem-mouse-brain.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1917.1278.0,0,1917,1280.file/multisem-mouse-brain.jpg"})
![マウス脳切片、最高取得速度1.22ギガピクセル/秒。 マウス脳切片、最高取得速度1.22ギガピクセル/秒。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.100.67.file/multisem-mouse-brain.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.360.240.file/multisem-mouse-brain.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.768.512.file/multisem-mouse-brain.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1024.683.file/multisem-mouse-brain.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1280.853.file/multisem-mouse-brain.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1440.960.file/multisem-mouse-brain.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/multisem-mouse-brain.jpg/_jcr_content/renditions/original./multisem-mouse-brain.jpg"})
マウス脳切片、最高取得速度1.22ギガピクセル/秒。
ご提供:J. Lichtman, Harvard University, Cambridge, MA, USA
マウス脳切片、最高取得速度1.22ギガピクセル/秒。
ご提供:J. Lichtman, Harvard University, Cambridge, MA, USA
かってない速度での大型組織切片の走査型電子顕微鏡観察
試料の広範囲をイメージングするため、ZEISSは新たなマルチビームSEM技術であるZEISS MultiSEMシリーズを開発しました。試料調整を自動化することで、MultiSEMはアレイトモグラフィー解析による超高分解能3Dデータ取得の劇的な高速化に成功しました。コネクトミクス解析に使われるような大きな脳組織(1 mm³)などの高分解能マッピングが可能となりました。
クライオFE-SEMを使用した実際に近い構造と機能の観察
試料の本来の形態を観察するには、化学固定ではなく凍結が必要です。凍結試料を使ったワークフローに適応するZEISSの電界放出型SEM(FE-SEM)と集束イオンビームSEM(FIB-SEM)により、繊細な試料に対する優れた低電圧イメージングが可能になります。ZEISSが開発したCorrelative Cryo Workflowは、ワイドフィールド顕微鏡、レーザー走査型顕微鏡、FIB-SEMをシームレスに組み合わせ、使いやすい手順にしたものです。
![マウス舌の断面像、SEMの可変圧力モードでイメージング。 マウス舌の断面像、SEMの可変圧力モードでイメージング。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.100.75.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.360.270.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.768.576.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1024.768.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1280.960.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1440.1080.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1920.1440.0,0,2048,1536.file/sen_mouse-tongue_evo.jpg"})
![マウス舌の断面像、SEMの可変圧力モードでイメージング。 マウス舌の断面像、SEMの可変圧力モードでイメージング。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.100.75.file/sen_mouse-tongue_evo.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.360.270.file/sen_mouse-tongue_evo.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.768.576.file/sen_mouse-tongue_evo.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1024.768.file/sen_mouse-tongue_evo.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1280.960.file/sen_mouse-tongue_evo.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1440.1080.file/sen_mouse-tongue_evo.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/ultrastructural-imaging/sen_mouse-tongue_evo.jpg/_jcr_content/renditions/original.image_file.1920.1440.file/sen_mouse-tongue_evo.jpg"})
マウス舌の断面像、SEMの可変圧力モードでイメージング。
ご提供:R. Reimer, Heinrich Pette Institute, Germany
マウス舌の断面像、SEMの可変圧力モードでイメージング。
ご提供:R. Reimer, Heinrich Pette Institute, Germany
トポグラフィーイメージング
ZEISS EVOのようなSEMやZEISS SigmaのようなFE-SEMを使うことで、コーティング処理や複雑な試料調整をすることなく、様々な試料の表面形態の卓越したイメージングが可能です。コーティング処理をしていない昆虫や骨試料、水分を含む試料、グリッド切片、樹脂包埋ブロック、陶化試料を撮影できます。可変圧力によりチェンバー内の環境を制御し、低真空で試料のイメージングを行えます。
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