![蔡司3View超薄切片机]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.100.100.477,0,1556,1079.file/sensebsd_product-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.360.360.477,0,1556,1079.file/sensebsd_product-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.768.768.477,0,1556,1079.file/sensebsd_product-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.477,0,1556,1079.file/sensebsd_product-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.1079.1079.477,0,1556,1079.file/sensebsd_product-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.1079.1079.477,0,1556,1079.file/sensebsd_product-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.1079.1079.477,0,1556,1079.file/sensebsd_product-stage.jpg"} "蔡司3View超薄切片机")
蔡司Sense BSD 使用SEM实现TEM级别的成像
透射电子显微镜（TEM）一直是超微结构成像的优选，但配备背散射电子探测器的扫描电子显微镜也可以获取类似TEM的高分辨率图像。蔡司Sense BSD可以在高效率和高质量图像的基础上，实现高分辨率超微结构成像，使您的SEM可以进行TEM级别的成像。
![Tricellaria inopinata的超微结构]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.100.100.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.360.360.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.768.768.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg"} "Tricellaria inopinata的超微结构")
Tricellaria inopinata的超微结构
以全新的速度和质量对超微结构进行成像
凭借出色的探测器灵敏度，Sense BSD可以检测到少量电子并将低信号转换为高衬度图像，因此得以用低加速电压和低电子剂量实现快速图像采集。您的生物样品可以在不受损的情况下成像，并防止因荷电效应引起的图像质量下降。
图片说明：Tricellaria inopinata的超微结构。样品由挪威卑尔根大学萨斯海洋分子生物学研究中心的Harald Hausen提供。
![从斑马鱼中分离出的免疫细胞，使用蔡司GeminiSEM和蔡司Sense BSD成像（1.5 kV，88 pA，像素大小：3 nm，驻留时间：1.6 µs，载物台偏置）。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.100.100.file/zebrafish-immune-cells-sense-bsd.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.360.360.file/zebrafish-immune-cells-sense-bsd.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.768.768.file/zebrafish-immune-cells-sense-bsd.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg"} "从斑马鱼中分离出的免疫细胞，使用蔡司GeminiSEM和蔡司Sense BSD成像（1.5 kV，88 pA，像素大小：3 nm，驻留时间：1.6 µs，载物台偏置）。")
从斑马鱼中分离出的免疫细胞，使用蔡司GeminiSEM和蔡司Sense BSD成像（1.5 kV，88 pA，像素大小：3 nm，驻留时间：1.6 µs，载物台偏置）。
单幅二维图像的采集
蔡司Sense BSD专为高分辨率、高衬度的二维图像采集设计。低加速电压和低电子剂量在防止电子束对生物样品造成损伤的同时，仍能清楚地显示组织或细胞的超微结构。同样，低光毒性的激发条件可以防止会使图像质量下降的荷电效应。
图片说明：从斑马鱼中分离出的免疫细胞，使用蔡司GeminiSEM和蔡司Sense BSD成像（1.5 kV，88 pA，像素大小：3 nm，驻留时间：1.6 µs，载物台偏置）。
![小鼠海马区图像，使用GeminiSEM和Sense BSD获取（1.5 kV，像素大小：1 nm，驻留时间：2 µs）。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.100.50.file/mouse-hippocampus-array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.360.180.file/mouse-hippocampus-array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.768.384.file/mouse-hippocampus-array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.1024.512.file/mouse-hippocampus-array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.1280.640.file/mouse-hippocampus-array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.1440.720.file/mouse-hippocampus-array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original./mouse-hippocampus-array-tomography.jpg"} "小鼠海马区图像，使用GeminiSEM和Sense BSD获取（1.5 kV，像素大小：1 nm，驻留时间：2 µs）。")
使用Array Tomography采集三维数据集
Array Tomography是可以对树脂包埋的生物样品连续切片进行成像，然后从图像系列中重构三维数据集的方法。蔡司Sense BSD出色匹配了生物样品切片成像的要求：高探测器灵敏度可实现低电压成像并防止样品损坏，同时，由于生成高衬度图像所需的背散射电子更少，还能实现快速成像。
图片说明：小鼠海马区图像，使用GeminiSEM和Sense BSD获取（1.5 kV，像素大小：1 nm，驻留时间：2 µs）。样品由加州大学圣地亚哥分校国家显微镜和成像研究中心的Mark H. Ellisman提供。
相关应用
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蔡司Sense BSD
用于对超微结构进行快速温和成像的背散射电子探测器
文件大小: 6 MB
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![蔡司Sense BSD的预览图像]({"max":"https://asset-downloads.zeiss.com/catalogs/preview/mic/be8f5326-ae42-4ad1-bfc1-860b6e54d883/CN_product-flyer_Sense_BSD_Acquiring_TEM-like_images_with_an_SEM.pdf.jpg"} "下载蔡司Sense BSD")
![蔡司Crossbeam——专为高效率三维分析和样品制备量身打造的FIB-SEM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.100.100.file/crossbeam-550-system.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.360.360.file/crossbeam-550-system.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.768.768.file/crossbeam-550-system.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/crossbeam-550-system.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/crossbeam-550-system.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/crossbeam-550-system.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/fib-sem/crossbeam/crossbeam-550-system.jpg/_jcr_content/renditions/original./crossbeam-550-system.jpg"} "蔡司Crossbeam")
![蔡司GeminiSEM系列]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.100.100.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.360.360.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.768.768.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1280.1280.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1353.1353.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/geminisem/zeiss_gemini-sem-stage.jpg/_jcr_content/renditions/original.image_file.1353.1353.285,86,1638,1439.file/zeiss_gemini-sem-stage.jpg"} "蔡司GeminiSEM系列")
![蔡司Sigma]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.100.100.420,0,1500,1080.file/sigma360-stage.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.360.360.420,0,1500,1080.file/sigma360-stage.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.768.768.420,0,1500,1080.file/sigma360-stage.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1024.1024.420,0,1500,1080.file/sigma360-stage.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sigma/sigma360-stage.png/_jcr_content/renditions/original.image_file.1080.1080.420,0,1500,1080.file/sigma360-stage.png"} "蔡司Sigma")
![ZEN Correlative Array Tomography]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.100.100.file/correlative-array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.360.360.file/correlative-array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.768.768.file/correlative-array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/correlative-array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/correlative-array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/correlative-array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/software/ZEN%20Correlative%20Array%20Tomography/correlative-array-tomography.jpg/_jcr_content/renditions/original./correlative-array-tomography.jpg"} "ZEN Correlative Array Tomography")