![ZEISS 3Viewウルトラミクロトーム ZEISS 3Viewウルトラミクロトーム]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.100.100.477,0,1556,1079.file/sensebsd_product-stage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.360.360.477,0,1556,1079.file/sensebsd_product-stage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.768.768.477,0,1556,1079.file/sensebsd_product-stage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.1024.1024.477,0,1556,1079.file/sensebsd_product-stage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.1079.1079.477,0,1556,1079.file/sensebsd_product-stage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.1079.1079.477,0,1556,1079.file/sensebsd_product-stage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/sensebsd_product-stage.jpg/_jcr_content/renditions/original.image_file.1079.1079.477,0,1556,1079.file/sensebsd_product-stage.jpg"})
ZEISS Sense BSD SEMでTEMレベルのイメージングを実現
これまで超微細構造イメージングには透過型電子顕微鏡(TEM)が推奨されてきましたが、後方散乱電子検出器を搭載した走査型電子顕微鏡(SEM)でも、高解像度でTEM並みの画像を取得することができます。ZEISS Sense BSDを、これまでにない高効率かつ高画質の高分解能超微細構造イメージングと組み合わせることで、お持ちのSEMでTEMレベルのイメージングが可能となります。
![コケムシ類(Tricellaria inopinata)の微細構造。 コケムシ類(Tricellaria inopinata)の微細構造。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.100.100.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.360.360.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.768.768.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg"})
![コケムシ類(Tricellaria inopinata)の微細構造。 試料ご提供: Harald Hausen, Sars Centre for Marine Molecular Biology, University of Bergen, Norway コケムシ類(Tricellaria inopinata)の微細構造。 試料ご提供: Harald Hausen, Sars Centre for Marine Molecular Biology, University of Bergen, Norway]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.100.100.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.360.360.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original.image_file.768.768.file/tricellaria-inopinata-ultrastructure-sense-bsd.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/tricellaria-inopinata-ultrastructure-sense-bsd.jpg/_jcr_content/renditions/original./tricellaria-inopinata-ultrastructure-sense-bsd.jpg"})
コケムシ類(Tricellaria inopinata)の微細構造。
これまでにない速度と品質で微細構造をイメージング
Sense BSDは優れた検出器感度によって非常に少数の電子を検出し、低信号を高コントラスト画像に変換します。また低加速電圧と低電子線量での高速画像取得が可能で、生体試料を損傷することなくイメージングでき、帯電効果による画質の低下を防止します。
キャプション:コケムシ類(Tricellaria inopinata)の微細構造。試料ご提供: Harald Hausen, Sars Centre for Marine Molecular Biology, University of Bergen, Norway
![ZEISS GeminiSEMおよびZEISS Sense BSDでゼブラフィッシュから単離した免疫細胞をイメージング(1.5 kV、88 pA、ピクセルサイズ:3 nm、画素滞在時間:1.6 µs、ステージバイアス)。 ZEISS GeminiSEMおよびZEISS Sense BSDでゼブラフィッシュから単離した免疫細胞をイメージング(1.5 kV、88 pA、ピクセルサイズ:3 nm、画素滞在時間:1.6 µs、ステージバイアス)。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.100.100.file/zebrafish-immune-cells-sense-bsd.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.360.360.file/zebrafish-immune-cells-sense-bsd.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.768.768.file/zebrafish-immune-cells-sense-bsd.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg"})
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.100.100.file/zebrafish-immune-cells-sense-bsd.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.360.360.file/zebrafish-immune-cells-sense-bsd.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original.image_file.768.768.file/zebrafish-immune-cells-sense-bsd.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/zebrafish-immune-cells-sense-bsd.jpg/_jcr_content/renditions/original./zebrafish-immune-cells-sense-bsd.jpg"})
ZEISS GeminiSEMおよびZEISS Sense BSDでゼブラフィッシュから単離した免疫細胞をイメージング(1.5 kV、88 pA、ピクセルサイズ:3 nm、画素滞在時間:1.6 µs、ステージバイアス)。
単一2D画像の取得
ZEISS Sense BSDは、高解像度・高コントラスト比の2D画像取得用に設計されており、低加速電圧と低電子線量が生体試料のビーム損傷を防ぐとともに、組織または細胞の微細構造を明らかにします。同様に、穏やかな励起状態は、画質低下の原因となる帯電の影響を防ぎます。
キャプション:ZEISS GeminiSEMおよびZEISS Sense BSDでゼブラフィッシュから単離した免疫細胞をイメージング(1.5 kV、88 pA、ピクセルサイズ:3 nm、画素滞在時間:1.6 µs、ステージバイアス)。
![GeminiSEMおよびSense BSDでマウス海馬をイメージング(1.5 kV、ピクセルサイズ:1 nm、画素滞在時間:2 µs)。 GeminiSEMおよびSense BSDでマウス海馬をイメージング(1.5 kV、ピクセルサイズ:1 nm、画素滞在時間:2 µs)。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.100.50.file/mouse-hippocampus-array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.360.180.file/mouse-hippocampus-array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.768.384.file/mouse-hippocampus-array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.1024.512.file/mouse-hippocampus-array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.1280.640.file/mouse-hippocampus-array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original.image_file.1440.720.file/mouse-hippocampus-array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/electron-ion/sem/sense-bsd/mouse-hippocampus-array-tomography.jpg/_jcr_content/renditions/original./mouse-hippocampus-array-tomography.jpg"})
アレイトモグラフィーでの3Dデータセット取得
アレイトモグラフィーは樹脂に埋め込まれた生体試料の連続切片をイメージングし、画像シリーズから3Dデータセットを再構築します。生体試料切片のイメージング要件に完全に適合するZEISS Sense BSDの高感度検出器によって低加速電圧イメージングが可能になり、試料の損傷が防止できます。また、少ない後方散乱電子でも高コントラスト画像を取得可能なため、高速イメージングが実現します。
キャプション:GeminiSEMおよびSense BSDでマウス海馬をイメージング(1.5 kV、ピクセルサイズ:1 nm、画素滞在時間:2 µs)。試料ご提供:Mark H. Ellisman, National Center for Microscopy and Imaging Research, University of California San Diego