![Array Tomographyのアプリケーション Courtesy of D. Sherrier, J. Caplan, and S. Modla, University of Delaware, USA Array TomographyのアプリケーションCourtesy of D. Sherrier, J. Caplan, and S. Modla, University of Delaware, USA]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-array-tomography-application-examples-stage-image_4.jpg/_jcr_content/renditions/original.image_file.100.56.file/zeiss-array-tomography-application-examples-stage-image_4.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-array-tomography-application-examples-stage-image_4.jpg/_jcr_content/renditions/original.image_file.360.203.file/zeiss-array-tomography-application-examples-stage-image_4.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-array-tomography-application-examples-stage-image_4.jpg/_jcr_content/renditions/original.image_file.768.432.file/zeiss-array-tomography-application-examples-stage-image_4.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-array-tomography-application-examples-stage-image_4.jpg/_jcr_content/renditions/original.image_file.1024.576.file/zeiss-array-tomography-application-examples-stage-image_4.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-array-tomography-application-examples-stage-image_4.jpg/_jcr_content/renditions/original.image_file.1280.720.file/zeiss-array-tomography-application-examples-stage-image_4.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-array-tomography-application-examples-stage-image_4.jpg/_jcr_content/renditions/original.image_file.1440.810.file/zeiss-array-tomography-application-examples-stage-image_4.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-array-tomography-application-examples-stage-image_4.jpg/_jcr_content/renditions/original./zeiss-array-tomography-application-examples-stage-image_4.jpg"})
Array Tomographyのアプリケーション
革新的な事例を見る
Array Tomography(AT)は、ナノスケールの分解能で3Dデータセットを生成する強力なアプローチです。試料は数百の切片にセクショニングされ、各切片が走査電子顕微鏡を使用して高分解能でイメージングされます。
非破壊的な方法として、試料の超薄切片をイメージング用基板上に置き、蛍光顕微鏡法などの手法やその他の解析アプローチを用いて観察します。
ボリュームEM(vEM)法には特別な装置が必要ないため、走査電子顕微鏡(SEM)とウルトラミクロトームがあればどのようなラボでもボリュームEMを活用できます。このような特徴を備えたATは、例からも分かるように、細胞から組織、植物に至るまで多様な試料の観察に有益なアプローチです。
脳組織の神経ネットワークを理解する
数百万の神経ネットワークを調べることで、脳のシグナル伝達経路に関する理解が深まります。
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg/_jcr_content/renditions/original.image_file.100.75.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg/_jcr_content/renditions/original.image_file.360.270.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg/_jcr_content/renditions/original.image_file.768.576.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg/_jcr_content/renditions/original.image_file.1024.768.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_1_4-3.jpg"})
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ATを用いて取得したサルの脳のオーバービュー画像。
ATを用いて取得したサルの脳のオーバービュー画像。
脳は、数百万の神経ネットワークとシグナル伝達経路を有する複雑な器官です。神経組織の構造と機能との関係を理解することは、この複雑さを部分的に解明するのに役立ち、脳の働きを深く理解することにつながるだけでなく、長期的な機能不全の医療介入による治療法の解明にも役立ちます。
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original.image_file.100.63.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original.image_file.360.225.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original.image_file.768.480.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original.image_file.1024.640.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg"})
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original.image_file.100.63.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original.image_file.360.225.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original.image_file.768.480.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original.image_file.1024.640.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_3_16-10.jpg"})
SEMを用いて取得したサルの脳の高解像度画像。
SEMを用いて取得したサルの脳の高解像度画像。
数百万の神経ネットワークを調べるには、高分解能3Dイメージングが必要です。小さな脳の試料では、全体の3Dデータセットを取得するのに時間がかかりますが可能な範囲です。ただし、マウスやサルの脳などの非常に大きな組織の場合は、イメージングをスケールアップさせて、現実的な時間枠の中で高分解能3Dデータセットを生成する必要があります。ATなら、これを実現できます。
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg/_jcr_content/renditions/original.image_file.100.75.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg/_jcr_content/renditions/original.image_file.360.270.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg/_jcr_content/renditions/original.image_file.768.576.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg/_jcr_content/renditions/original.image_file.1024.768.file/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_monkey-brain_atlas-5_2_4-3.jpg"})
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ATを用いて取得したサルの脳血管。
ATを用いて取得したサルの脳血管。
ZEISS Atlas 5 Array Tomographyは、基板上の連続する切片をナノメーターレベルの分解能で自動的にイメージングするハードウェア/ソフトウェアツールです。この独自のワークフローは使いやすいだけでなく、特に自動イメージング用に設計されており、脳組織の膨大な結合を解明するのに必要となる大容量の3Dビジュアライゼーションを可能にします。
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自動で取得した、脳血管を示すサルの脳の拡大画像。Atlas 5 Array Tomographyを用いて取得した画像。実視野:3700 mm
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_2_1-1.jpg"})
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1000を超える画像をつなぎ合わせたサルの脳を表すモザイク画像。各タイル画像は4096 x 4096ピクセル(ピクセルサイズ150 nm)。
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg/_jcr_content/renditions/original./zeiss-microscopy-array-tomography_screenshot_stitching_monkey-brain_atlas-5_1_1-1.jpg"})
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タイル画像の自動スティッチングにより作成した、サルの脳の広視野画像。
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.100.75.0,0,1346,1010.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.360.270.0,0,1346,1010.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.768.576.0,0,1346,1010.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.1024.768.0,0,1346,1010.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.1280.960.0,0,1346,1010.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.1346.1010.0,0,1346,1010.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.1346.1010.0,0,1346,1010.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg"})
マウス脳の超薄切片
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.100.75.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.360.270.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.768.576.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.1024.768.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.1280.960.file/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy_roi_cerebellum-atlas-array-tomography_4-3.jpg"})
マウス脳の超薄切片。試料ご提供:J. Lichtman, Harvard University, USA
マウス脳の超薄切片。試料ご提供:J. Lichtman, Harvard University, USA
Atlas 5 Array Tomographyのコンピューター支援ツールを使うことにより、何百もの連続する切片上におけるあらゆる形状の関心領域を制限なく定義できます。これは特に、サルまたはマウスの脳の神経ネットワークを捉える実験等で役立ちます。
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.100.75.file/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.360.270.file/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.768.576.file/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.1024.768.file/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg"})
マウス脳の超薄切片
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.100.75.file/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.360.270.file/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.768.576.file/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original.image_file.1024.768.file/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem-mouse-brain-atlas-array-tomography_4-3.jpg"})
マウス脳の超薄切片。試料ご提供:J. Lichtman, Harvard University, USA
マウス脳の超薄切片。試料ご提供:J. Lichtman, Harvard University, USA
ここに示すのは、基板に載せたマウス脳の超薄切片の拡大SEM画像です。画像の自動取得をサポートするAtlas 5 Array Tomographyによって撮影されました。
核やミトコンドリアなどの細胞内の詳細構造が特定でき、さらに3Dで再構成されると、異なるニューロン間の結合がマッピングされます。
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.100.63.0,0,1370,856.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.360.225.0,0,1370,856.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.768.480.0,0,1370,856.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.1024.640.0,0,1370,856.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.1280.800.0,0,1370,856.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.1370.856.0,0,1370,856.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.1370.856.0,0,1370,856.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg"})
マウス脳の超薄切片
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.100.62.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.360.225.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.768.480.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.1024.640.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original.image_file.1280.800.file/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem-cerebellum-atlas-array-tomography_16-10.jpg"})
マウス脳の超薄切片。試料ご提供:J. Lichtman, Harvard University, USA
マウス脳の超薄切片。試料ご提供:J. Lichtman, Harvard University, USA
この画像は、テープでマウントされ、イメージングの準備ができた多数の脳切片を示しています。Atlas 5 Array Tomographyは、後の高分解能SEMによる取得のために小脳の各切片を特定し、各切片の有益な情報すべてをソフトウェアプラットフォームに保存します。
この動画は、Atlas 5 Array Tomographyを使用してATのワークフローを効率化することがもたらす大きな価値を示しています。動画が進むにつれて、各切片から取得された高分解能データがどのように組み合わされ、最終的に3Dデータセットを生成するかを見ることができます。
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original./zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg"})
マウス脳の超薄切片
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg/_jcr_content/renditions/original./zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_1-1.jpg"})
高分解能SEMで視神経を捉えるには、各切片をイメージングしてその後再構成する必要があります。Array Tomographyは各切片の位置を自動で特定し、その後イメージングするため、迅速でシンプルな取得プロセスが実現します。
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original./zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg"})
マウス脳の超薄切片
![マウス脳の超薄切片 マウス脳の超薄切片]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.100.100.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.360.360.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.768.768.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg/_jcr_content/renditions/original./zeiss-microscopy_roi_optic-nerve-atlas-array-tomography_2_16-10_2.jpg"})
この画像は、Atlas 5ソフトウェアを使用してイメージングする前の、ウェハ上に載せられた視神経の各切片を示しています。
Array Tomographyを用いて脳をイメージングする際は、数百、あるいは数千もの切片の位置を特定してイメージングする必要があります。この切片をそれぞれ手動で特定するのは大変な作業であり、あまりにも時間がかかります。Atlas 5は、各切片を自動で素早く特定するため、その後のイメージングを素早く効率的に開始でき、各切片のすべての高分解能情報がソフトウェアに登録されます。
植物の健康状態に対する根粒内の細菌の影響を理解する
Correlative Array Tomography(CAT)を用いた蛍光および構造データのオーバーレイにより、根粒と細菌の分布を視覚化できます。
![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.100.56.1,0,1334,750.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.360.203.1,0,1334,750.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.768.432.1,0,1334,750.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.1024.576.1,0,1334,750.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.1280.720.1,0,1334,750.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.1333.750.1,0,1334,750.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.1333.750.1,0,1334,750.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg"})
プラスモデスマータの分布を示す根粒
![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.100.56.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.360.202.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.768.432.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.1024.576.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original.image_file.1280.720.file/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original./zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg/_jcr_content/renditions/original./zeiss-microscopy_3d_root-nodules-plasmodesmata_cat_19-9.jpg"})
プラスモデスマータの分布を示す根粒。ご提供:D. Sherrier, J. Caplan, and S. Modla, University of Delaware, USA
プラスモデスマータの分布を示す根粒。ご提供:D. Sherrier, J. Caplan, and S. Modla, University of Delaware, USA
植物の根は、ネットワークを張り巡らせることで、すべての植物の成長に欠かせない水や栄養にアクセスします。植物の健康や産出量を最適化するには、根のネットワーク全体を調べ、外部の微生物の影響を理解することが重要です。
植物と根粒内の細菌における共生関係を調べるにあたっては、根粒と細菌分布に関する知識が必要であり、これを詳細に理解するためには、蛍光および高分解能の構造評価を組み合わせることが必須となります。
Correlative Array Tomographyでは蛍光および構造データにおける両方のオーバーレイが可能なため、根粒と細菌分布を視覚化することができます。
根粒内の細菌が植物の状態に与える影響をきちんと理解するためには、高分解能の構造情報を精度の高い蛍光情報と組み合わせることが重要です。
![]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg/_jcr_content/renditions/original.image_file.100.63.0,0,1731,1082.file/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg/_jcr_content/renditions/original.image_file.360.225.0,0,1731,1082.file/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg/_jcr_content/renditions/original.image_file.768.480.0,0,1731,1082.file/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg/_jcr_content/renditions/original.image_file.1024.640.0,0,1731,1082.file/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg/_jcr_content/renditions/original.image_file.1280.800.0,0,1731,1082.file/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg/_jcr_content/renditions/original.image_file.1440.900.0,0,1731,1082.file/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg/_jcr_content/renditions/original.image_file.1731.1082.0,0,1731,1082.file/zeiss-microscopy_lm_3d-data-reconstruction_cat_16-10.jpg"})
プラスモデスマータの分布を示す根粒。ご提供:D. Sherrier, J. Caplan, and S. Modla, University of Delaware, USA
これらの画像は、根粒の連続する切片から得られた蛍光データの3D再構成または1枚の切片であり、プラスモデスマータの分布を示しています。SEMデータを用いてスタックをオーバーレイすることで、細菌感染とプラスモデスマータの分布との関係を調べることができます。
試料はEponで包埋し、ウルトラミクロトームで切断しています。連続する切片を、マイクロマニピュレーターでITOコーティングしたカバーガラスに移動しました。細胞壁はCalcofluor White(青)で、プラスモデスマータはカロースに対してAlexa Fluor 647で染色されました。SEMでのイメージングのため、試料は後染色されています。
![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,999,562.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,999,562.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,999,562.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.999.562.0,0,999,562.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.999.562.0,0,999,562.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.999.562.0,0,999,562.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.999.562.0,0,999,562.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg"})
プラスモデスマータの分布を示す根粒
![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.100.56.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.360.203.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original.image_file.768.432.file/zeiss-microscopy_3d_correlated-data-stack_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original./zeiss-microscopy_3d_correlated-data-stack_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original./zeiss-microscopy_3d_correlated-data-stack_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original./zeiss-microscopy_3d_correlated-data-stack_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_3d_correlated-data-stack_cat.jpg/_jcr_content/renditions/original./zeiss-microscopy_3d_correlated-data-stack_cat.jpg"})
![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1291,726.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1291,726.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1291,726.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1291,726.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg/_jcr_content/renditions/original.image_file.1280.720.0,0,1291,726.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg/_jcr_content/renditions/original.image_file.1291.726.0,0,1291,726.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg/_jcr_content/renditions/original.image_file.1291.726.0,0,1291,726.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-3_cat_16-9.jpg"})
プラスモデスマータの分布を示す根粒
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プラスモデスマータの分布を示す根粒
![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg/_jcr_content/renditions/original.image_file.100.62.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg/_jcr_content/renditions/original.image_file.360.225.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg/_jcr_content/renditions/original.image_file.768.480.file/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem_plasmodesmata-root-nodules-2_cat_16-10.jpg"})
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プラスモデスマータの分布を示す根粒
![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg/_jcr_content/renditions/original.image_file.100.56.file/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg/_jcr_content/renditions/original.image_file.360.203.file/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg/_jcr_content/renditions/original.image_file.768.432.file/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg/_jcr_content/renditions/original.image_file.1024.576.file/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg/_jcr_content/renditions/original.image_file.1280.720.file/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg/_jcr_content/renditions/original./zeiss-microscopy_sem_plasmodesmata-root-nodules_cat.jpg"})
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プラスモデスマータの分布を示す根粒
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![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1447,814.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1447,814.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1447,814.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1447,814.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.1280.720.0,0,1447,814.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.1440.810.0,0,1447,814.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.1447.814.0,0,1447,814.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg"})
プラスモデスマータの分布を示す根粒
![プラスモデスマータの分布を示す根粒 プラスモデスマータの分布を示す根粒]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.100.56.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.360.203.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.768.432.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.1024.576.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.1280.720.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original.image_file.1440.810.file/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg/_jcr_content/renditions/original./zeiss-microscopy_serial-sections_root-nodules_atlas-5-array-tomography_1.jpg"})
Array Tomographyによる画像取得の設定方法
この動画では、ZEISS Atlas 5 Array Tomographyを使用して、蛍光およびSEMデータとの相関データセットを生成するワークフローを説明します。動画内で示される例の1つは酵母試料で、この酵母の構成を示しています。
動画内の酵母試料はEponで包埋後、ウルトラミクロトームで切断されています。連続する切片を、マイクロマニピュレーターでITOコーティングしたカバーガラスに移動しました。試料はまず光学顕微鏡でイメージングされ、その後SEMによるイメージングのために後染色されました。
ご提供:D. Sherrier, J. Caplan, and S. Modla, University of Delaware, USA
ハンチントン病の疾患生物学と進行を理解する
Correlative Array Tomography(CAT)を用いてマクロファージ内のタンパク質プラークの発達を視覚化できます。
![変異型ハンチンチンタンパク質の凝集により誘発されたタンパク質プラークを示すマクロファージ 変異型ハンチンチンタンパク質の凝集により誘発されたタンパク質プラークを示すマクロファージ]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.100.63.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.360.225.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.768.480.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.1024.640.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.1280.800.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.1440.900.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original./fluorescence_huntingtin-protein-plaque-formation_cat.jpg"})
変異型ハンチンチンタンパク質の凝集により誘発されたタンパク質プラークを示すマクロファージ
![変異型ハンチンチンタンパク質の凝集により誘発されたタンパク質プラークを示すマクロファージ 変異型ハンチンチンタンパク質の凝集により誘発されたタンパク質プラークを示すマクロファージ]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.100.63.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.360.225.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.768.480.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.1024.640.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.1280.800.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original.image_file.1440.900.file/fluorescence_huntingtin-protein-plaque-formation_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/fluorescence_huntingtin-protein-plaque-formation_cat.jpg/_jcr_content/renditions/original./fluorescence_huntingtin-protein-plaque-formation_cat.jpg"})
変異型ハンチンチンタンパク質の凝集により誘発されたタンパク質プラークを示すマクロファージ。この画像は、1つの切片からの蛍光データセットで、マクロファージ内のタンパク質プラークを示しています。DNA:青(DAPI)、ハンチンチンタンパク質:赤(Alexa Fluor 647)。ご提供:Jeff Caplan, University of Delaware, USA
変異型ハンチンチンタンパク質の凝集により誘発されたタンパク質プラークを示すマクロファージ。この画像は、1つの切片からの蛍光データセットで、マクロファージ内のタンパク質プラークを示しています。DNA:青(DAPI)、ハンチンチンタンパク質:赤(Alexa Fluor 647)。ご提供:Jeff Caplan, University of Delaware, USA
ハンチントン病は、ハンチンチンタンパク質をコーディングする4番染色体上の遺伝子の欠陥によって生じる不治で進行性の神経変性疾患です。欠陥遺伝子は変異型ハンチンチンタンパク質の形成をもたらします。変異型ハンチンチンタンパク質はミスフォールドして凝集し、脳内でタンパク質プラークの発達を引き起こします。このような脳の変質により、運動、行動、思考、感情の混乱が生じます。
![ハンチンチンの過剰発現の結果、凝集したタンパク質がZスタック内で明確に視認できる ハンチンチンの過剰発現の結果、凝集したタンパク質がZスタック内で明確に視認できる]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.100.63.0,0,1821,1138.file/lm_sem_z-stack-correlation_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.360.225.0,0,1821,1138.file/lm_sem_z-stack-correlation_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.768.480.0,0,1821,1138.file/lm_sem_z-stack-correlation_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.1024.640.0,0,1821,1138.file/lm_sem_z-stack-correlation_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.1280.800.0,0,1821,1138.file/lm_sem_z-stack-correlation_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.1440.900.0,0,1821,1138.file/lm_sem_z-stack-correlation_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.1821.1138.0,0,1821,1138.file/lm_sem_z-stack-correlation_cat.jpg"})
ハンチンチンの過剰発現の結果、凝集したタンパク質がZスタック内で明確に視認できる
![ハンチンチンの過剰発現の結果、凝集したタンパク質がZスタック内で明確に視認できる ハンチンチンの過剰発現の結果、凝集したタンパク質がZスタック内で明確に視認できる]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.100.62.file/lm_sem_z-stack-correlation_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.360.225.file/lm_sem_z-stack-correlation_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.768.480.file/lm_sem_z-stack-correlation_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.1024.640.file/lm_sem_z-stack-correlation_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.1280.800.file/lm_sem_z-stack-correlation_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original.image_file.1440.900.file/lm_sem_z-stack-correlation_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_z-stack-correlation_cat.jpg/_jcr_content/renditions/original./lm_sem_z-stack-correlation_cat.jpg"})
ハンチンチンの過剰発現の結果、凝集したタンパク質がZスタック内で明確に視認できます。GFP-Huntington抗体を使用して、細胞内のハンチンチンタンパク質のプラークの位置を特定しました(Alexa Fluor 647、赤)。核は青で示されています(Hoechst)。ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関。ご提供:J. Caplan, E. Kmiec and S. Modla, University of Delaware, USA
ハンチンチンの過剰発現の結果、凝集したタンパク質がZスタック内で明確に視認できます。GFP-Huntington抗体を使用して、細胞内のハンチンチンタンパク質のプラークの位置を特定しました(Alexa Fluor 647、赤)。核は青で示されています(Hoechst)。ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関。ご提供:J. Caplan, E. Kmiec and S. Modla, University of Delaware, USA
マクロファージ内でのタンパク質プラークの発達(ハンチントン病の研究のモデル系)を視覚化することと、これらが微細構造の細胞細部にどのように関連しているかを理解することは、ハンチントン病の疾患生物学と進行の理解において重要です。
Correlative Array Tomographyにより、このマクロファージの連続切片から微細構造と蛍光データの両方を取得することができます。
![ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関 ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.100.63.file/lm_sem_3d-schematic-representation_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.360.225.file/lm_sem_3d-schematic-representation_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.768.480.file/lm_sem_3d-schematic-representation_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.1024.640.file/lm_sem_3d-schematic-representation_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.1280.800.file/lm_sem_3d-schematic-representation_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.1440.900.file/lm_sem_3d-schematic-representation_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original./lm_sem_3d-schematic-representation_cat.jpg"})
ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関
![ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関 ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.100.63.file/lm_sem_3d-schematic-representation_cat.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.360.225.file/lm_sem_3d-schematic-representation_cat.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.768.480.file/lm_sem_3d-schematic-representation_cat.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.1024.640.file/lm_sem_3d-schematic-representation_cat.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.1280.800.file/lm_sem_3d-schematic-representation_cat.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original.image_file.1440.900.file/lm_sem_3d-schematic-representation_cat.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/application-examples/lm_sem_3d-schematic-representation_cat.jpg/_jcr_content/renditions/original./lm_sem_3d-schematic-representation_cat.jpg"})
ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関。ご提供:J. Caplan, E. Kmiec and S. Modla, University of Delaware, USA
ハンチンチンプラークの分布と核の位置を3Dで示すLMおよびSEMのZスタックの相関。ご提供:J. Caplan, E. Kmiec and S. Modla, University of Delaware, USA
ハンチントン病の疾患生物学と進行をきちんと理解するには、微細構造および蛍光データの相関が重要です。
左の画像は、ハンチンチンタンパク質を発現しているマクロファージの連続LM画像とSEM画像を組み合わせて3Dで取得した情報を示しています。画像は、ZEISS ZEN Correlative Array Tomographyを用いた実験で取得しました。