ZEN Microscopy Software

Our novel AI chatbot in ZEN, currently available on LSM systems, has received significant updates:

• Supports multiple ZEN versions and answers version-specific
• Takes separate capabilities of LSM 910/990 into account
• Improved stability also for long answers
• Takes conversation history into account

The high-speed LSM Lightfield 4D imaging received important updates:

• Viluma 9 compatibility for fast multi-color imaging and high illumination power in the 500-600nm spectrum
• Maximum size of positions array increased to 1000
• Acceleration of Direct Processing by a factor of two
• Usability improvements in Lightfield processing

Lattice SIM received some highly anticipated usability upgrades:

• Automatic synchronization of the selected objective lens with all tracks of the experiment
• Smart setup allows defining dye names in the tracks

A number of small additions round up this release:

• Image analysis (2D/3D Toolkit): Improved user interface for selecting segmentation methods
• AI Toolkit: Improved import of multi-channel images for training of Intellesis Segmentation models
• File import: Glencoe Bioformats importer upgraded to the latest version 8.0.1-761
• LSM: Iterative Deconvolution (DCV) for confocal images, compatible with LSM Plus

Newly added features to Guided Acquisition enable advanced time-lapse imaging:

• Repeatedly loop over an experiment to dynamically find rare events.
• Create high-resolution time-lapse recordings of your favorite biologically processes.
• Save your sample from unnecessary bleaching and your disk space from unneeded data.

The Deep Learning segmentation workflow received important updates:

• Super-fast image annotations with Segment Anything (SAM) on arivis Cloud
• Simplified model management with new filter function in the ZEN model store

Channel definition and Smart Setup in ZEN are now based on a new spectral database:

• New database contains many modern dyes, especially for genetically-encoded fluorescent proteins.
• Database is maintained and updated by scientific community.
• Legacy spectra from old database remain available.
  

The Microscopy Copilot, your personal AI assistant, helps you to interactively discover new possibilities for your imaging experiments:

• Novice and advanced users get instant advice on how to optimize their acquisition setups.
• The chatbot considers your LSM system configuration.
  

Built on the established jDCV algorithm and Airyscan Multiplexing, this new processing option delivers superior image quality at high imaging speed and is compatible with Direct Processing and Batch Processing.

This new processing algorithm for Apotome datasets enables confocal-like image quality on widefield microscopes:

• Lateral resolution down to 180 nm 
• Increased axial resolution
• Increased signal-to-noise ratio (SNR)
• Fully integrated in ZEN Direct Processing & Batch Processing workflows
  

This new ZEN acquisition module enables NLO-FLIM (nonlinear optical fluorescence lifetime imaging microscopy) on ZEISS LSM 980 systems.

• Compatible with single- or dual-line excitation
• Single- or dual-channel non-descanned detection
  

This module extends the possibilities of ZEN lite – the free version of ZEN for basic image acquisition and analysis – to control microscopes with coded components:

• Readout, display, and adjustment of encoded microscope configurations in ZEN lite
• LED light source control with ZEN lite
  

ZEISS Viluma is a family of light sources for fluorescence imaging that provides you with all the benefits of advanced LED technology. With the integration of the new light sources in ZEN 3.11, you can unlock their full potential across the entire ZEN ecosystem and the ZEISS portfolio of widefield microscopes.

The ZEN 3.11 release continues the performance enhancement of Instance Segmentation:

• Faster time to result in all workflows that include Instance Segmentation
• Faster training speeds
• Faster model download between ZEISS arivis Cloud and ZEN
  

After optimizing the Deep Learning training performance in the previous version, this release now adds accelerated model employment. You will benefit from:

• Better time-to-result for Instance Segmentation​
• Faster inference also in Image Analysis Wizard, Bio Apps, and Guided Acquisition workflows
  

As a user of the Bio Apps Toolkit, you can now analyze nuclear translocation of molecules. The new method comes with:

• Simple, one-step setups​
• A dedicated result view including multi-well plate heatmaps​
• The option to integrate advanced Deep Learning segmentation
  

Lattice SIM processing has been implemented as a feature of Direct Processing. When performing SIM² processing workflows, you will benefit from:

• Improved time-to-result​
• Increased computational resources by parallel processing on a dedicated processing workstation​
• Intelligent data compression for faster file transfers
  

On widefield modalities, users of the AI Toolkit can now employ Noise2Void Deep Learning during Continuous Acquisition Mode. This enables:

• Efficient live denoising with best-in-class algorithm​
• Sample exploration with quality images under mild illumination settings​
• Reduction of phototoxicity and bleaching