ZEISS Lattice Lightsheet 7

Lattice Light Sheet Microscopy provides gentle, high-speed 3D imaging using structured light sheets to minimize phototoxicity and photobleaching. 3D cell culture models, including spheroids and organoids, better recapitulate tissue architecture and gradients than 2D cultures, enabling more physiologically relevant studies.

Single Molecule Localization Microscopy (SMLM) creates ultra-high-resolution images by detecting individual fluorescent molecules one at a time. By precisely locating each molecule and repeating the process millions of times, it builds a highly detailed image at the nanoscale. One widely used SMLM method is STORM, which uses stochastic activation of fluorophores to localize single molecules with nanometer precision. Each binding event produces a detectable signal that reveals the target's exact location. Together, these repeated events generate exceptionally detailed images of cellular structures.

Cell proliferation is a fundamental process for understanding tissue growth and disease progression, particularly in cancer. Studying its underlying mechanisms enables researchers to develop targeted therapies that help control abnormal cell growth.

^{Image courtesy: Abel Pereira da Graca, ZMCC Oberkochen, Germany}

T cells play a central role in the immune response by recognizing and eliminating infected or cancerous cells. Research into their mechanisms and function is driving the development of more effective immunotherapies, helping improve treatment outcomes for a wide range of diseases.

^{Image courtesy: Elisa Sanchez, Morgan Huse lab, Memorial Sloan Kettering Cancer Center, New York}

Wound healing assays provide a model for studying tissue repair and evaluating treatment effectiveness. By replicating the wound closure process, they enable researchers to investigate healing mechanisms and advance the development of therapies for skin injuries and related conditions.

^{Image courtesy: Abel Pereira da Graca, ZMCC Oberkochen, Germany}

Studying antibody uptake in 4D provides dynamic insights into antibody–cell interactions in a physiologically relevant 3D environment. Unlike endpoint assays, it distinguishes true cellular uptake from membrane retention and enables precise quantification over time. Lattice Lightsheet 7 helps optimize therapeutic antibody uptake, supporting improved drug design, delivery, and treatment efficacy.

4D time series of a U2OS cell from the start of exposure to Trastuzumab until 48hrs later, when antibody uptake had reached saturation. Trastuzumab was labelled with pHrodo Red. Arivis Pro was used to segment compartments with Trastuzumab uptake and color-coded for fluorescence intensity.

Studying antibody uptake in 4D provides dynamic insights into antibody–cell interactions in a physiologically relevant 3D environment. Unlike endpoint assays, it distinguishes true cellular uptake from membrane retention and enables precise quantification over time. Lattice Lightsheet 7 helps optimize therapeutic antibody uptake, supporting improved drug design, delivery, and treatment efficacy.

3D rendering of U2OS cell after 24hrs of exposure to Trastuzumab labelled with pHrodo Red (cyan) and Zenon Alexa647 (orange). pHrodo Red fluoresces in acidic environments such as endosomes and lysosomes, which are the intracellular compartments where internalized antibodies are trafficked.

Studying antibody uptake in 4D provides dynamic insights into antibody–cell interactions in a physiologically relevant 3D environment. Unlike endpoint assays, it distinguishes true cellular uptake from membrane retention and enables precise quantification over time. Lattice Lightsheet 7 helps optimize therapeutic antibody uptake, supporting improved drug design, delivery, and treatment efficacy.

Trastuzumab was labelled with pHrodo and added to U2OS cells. Antibody uptake was observed for 24hrs every 10min. Fluorescence signal was segmented in arivis Pro and color-coded for fluorescence intensity. IgG antibodies labelled with pHrodo served as negative control. Graphic illustrating Trastuzumab uptake over the period of 48hrs. Analysed with arivis Pro.

Lattice Lightsheet 7 enables real-time visualization of T cell dynamics, interactions, and immune responses, providing deeper insights into T cell function and therapeutic potential. Combined with multi-well plate compatibility, it supports screening and identification of promising genetically retargeted T cell candidates for next-generation immunotherapies.

T cells are stained with a calcium dye. Cancer cells are stained with Lifeact-mCherry, which appear red under the imaging light. Cells shown in pink indicate apoptosis.

^{Image courtesy of Ms. Kylie Luong, A/Prof Misty Jenkins, Niall Geoghegan, WEHI, Melbourne.}

The uptake of antibodies by cancer cells plays an important role in the effectiveness of antibody-based therapies for cancer and infectious diseases. Understanding these mechanisms can help optimize drug delivery and improve therapeutic outcomes in clinical applications.

Lattice Lightsheet 7 enables real-time visualization of T cell dynamics, interactions, and immune responses, providing deeper insights into T cell function and therapeutic potential. Combined with multi-well plate compatibility, it supports screening and identification of promising genetically retargeted T cell candidates for next-generation immunotherapies.

Cytotoxic T-Lymphocyte interacting with a mouse melanoma target cell. T-Lymphocyte is stained with Cell Vue Maroon (orange) and the target cell is expessing F-tractin EGFP (green)

^{Image courtesy: Elisa Sanchez, Morgan Huse lab, Memorial Sloan Kettering Cancer Center, New York}

Lattice Lightsheet 7 enables real-time visualization of T cell dynamics, interactions, and immune responses, providing deeper insights into T cell function and therapeutic potential. Combined with multi-well plate compatibility, it supports screening and identification of promising genetically retargeted T cell candidates for next-generation immunotherapies.

Cytotoxic T-Lymphocyte interacting with a mouse melanoma target cell. T-Lymphocyte is stained with Cell Vue Maroon (orange) and the target cell is expessing F-tractin EGFP (green)

^{Image courtesy: Elisa Sanchez, Morgan Huse lab, Memorial Sloan Kettering Cancer Center, New York}

Lattice Lightsheet 7 enables real-time visualization of T cell dynamics, interactions, and immune responses, providing deeper insights into T cell function and therapeutic potential. Combined with multi-well plate compatibility, it supports screening and identification of promising genetically retargeted T cell candidates for next-generation immunotherapies.

Cytotoxic T-Lymphocyte interacting with a mouse melanoma target cell. T-Lymphocyte is stained with Cell Vue Maroon (orange) and the target cell is expessing F-tractin EGFP (green)

^{Image courtesy: Elisa Sanchez, Morgan Huse lab, Memorial Sloan Kettering Cancer Center, New York}

Lattice Lightsheet 7 enables detailed 3D visualization of cell proliferation over time, revealing how cells interact, organize, migrate, and differentiate in response to stimuli. Combined with Arivis Pro’s AI-powered segmentation and tracking, researchers can automatically identify cells and nuclei, detect mitotic events, and follow cell divisions to distinguish mother and daughter cells, providing deeper insights into disease mechanisms and therapeutic development.

LLC-PK1 cells undergoing mitosis. Cells are expressing H2B-mCherry (magenta) and α-Tubulin mEGFP (white).

^{Data Acquisition and Analysis: Abel Pereira da Graca and Noemi Castroviejo Jimenez, ZM Customer Center Oberkochen, Germany.}

Lattice Lightsheet 7 enables detailed 3D visualization of cell proliferation over time, revealing how cells interact, organize, migrate, and differentiate in response to stimuli. Combined with Arivis Pro’s AI-powered segmentation and tracking, researchers can automatically identify cells and nuclei, detect mitotic events, and follow cell divisions to distinguish mother and daughter cells, providing deeper insights into disease mechanisms and therapeutic development.

LLC-PK1 cells undergoing mitosis. Cells are expressing H2B-mCherry (magenta) and α-Tubulin mEGFP (white).

^{Data Acquisition and Analysis: Abel Pereira da Graca and Noemi Castroviejo Jimenez, ZM Customer Center Oberkochen, Germany.}

Lattice Lightsheet 7 enables detailed 3D visualization of cell proliferation over time, revealing how cells interact, organize, migrate, and differentiate in response to stimuli. Combined with Arivis Pro’s AI-powered segmentation and tracking, researchers can automatically identify cells and nuclei, detect mitotic events, and follow cell divisions to distinguish mother and daughter cells, providing deeper insights into disease mechanisms and therapeutic development.

LLC-PK1 cells undergoing mitosis. Cells are expressing H2B-mCherry (magenta) and α-Tubulin mEGFP (white).

^{Data Acquisition and Analysis: Abel Pereira da Graca and Noemi Castroviejo Jimenez, ZM Customer Center Oberkochen, Germany.}

Lattice Lightsheet 7 enables real-time 3D visualization of cell migration and wound healing, capturing large volumes of dynamic cellular activity with high precision. This approach provides deeper insights into the mechanisms driving tissue repair by revealing how cells interact with their environment and respond during healing processes.

LLC-PK1 cells undergoing mitosis. Cells are expressing H2B-mCherry (magenta) and α-Tubulin mEGFP (white).

^{Data Acquisition and Analysis: Abel Pereira da Graca and Noemi Castroviejo Jimenez, ZM Customer Center Oberkochen, Germany.}

Lattice Lightsheet 7 enables real-time 3D visualization of cell migration and wound healing, capturing large volumes of dynamic cellular activity with high precision. This approach provides deeper insights into the mechanisms driving tissue repair by revealing how cells interact with their environment and respond during healing processes.

LLC-PK1 cells undergoing mitosis. Cells are expressing H2B-mCherry (magenta) and α-Tubulin mEGFP (white).

^{Data Acquisition and Analysis: Abel Pereira da Graca and Noemi Castroviejo Jimenez, ZM Customer Center Oberkochen, Germany.}

Lattice Lightsheet 7 enables precise, real-time 3D imaging of cell migration and wound healing, capturing dynamic cellular processes across large volumes. By visualizing cell behavior and interactions with the surrounding environment, it provides valuable mechanistic insights into tissue repair and the factors regulating migration and healing.

Cancer invasion assays elucidate how cancer cells invade tissues, offering insights into tumor progression and metastasis. They also evaluate therapeutic agents’ effectiveness in inhibiting invasion, guiding targeted treatment development.

Spheroids provide physiologically relevant models of tumor growth and cell interactions. Observing their formation can enhance the study of cancer biology and drug responses, leading to more accurate predictions of therapeutic efficacy in vivo.

Characterizing CIVMs is crucial as it enhances understanding of cellular interactions and the microenvironment, influencing disease progression and treatment. These models improve preclinical study reliability, leading to better therapeutic strategies and patient care.

^{Image courtesy: Bjorn Onfelt, KTH Royal Institute of Technology, Stockholm, Sweden}

Mapping specific markers within CIVMs using multiplex immunofluorescence is essential for identifying the presence, proportion, and function of different cell types. This step is crucial to ensure that these models accurately reflect the characteristics of tissues found in vivo.

^{Image courtesy: InSphero AG, Switzerland}

ZEISS Lattice Lightsheet 7 enables detailed analysis of cancer cell invasion by capturing cell migration, extracellular matrix remodeling, and metastatic behaviors in 3D with high reproducibility. This advanced imaging approach supports more reliable insights into tumor biology and helps accelerate drug discovery workflows.

Spheroids formed from U2OS cells expressing Actin-EGFP, infiltrating the extracellular matrix (collagen), imaged for 24hrs every 5min. Color-coded depth projection.

Text: ZEISS Lattice Lightsheet 7 enables detailed analysis of cancer cell invasion by capturing cell migration, extracellular matrix remodeling, and metastatic behaviors in 3D with high reproducibility. This advanced imaging approach supports more reliable insights into tumor biology and helps accelerate drug discovery workflows.

Spheroids formed from U2OS cells expressingActin-EGFP, infiltrating the extracellular matrix (collagen), imaged for 24hrs every 5min. Color-coded depth projection.

ZEISS Lattice Lightsheet 7 enables detailed analysis of cancer cell invasion by capturing cell migration, extracellular matrix remodeling, and metastatic behaviors in 3D with high reproducibility. This advanced imaging approach supports more reliable insights into tumor biology and helps accelerate drug discovery workflows.

Spheroids formed from U2OS cells expressing Actin-EGFP, infiltrating the extracellular matrix (collagen), imaged for 24hrs every 5min. Color-coded depth projection.

Lattice Lightsheet 7 enables imaging of differentorgans of developing zebrafish embryos with ease. Tail, brain and even heartbeat can be imaged with subcellular resolution and high volume speeds, catering for all your imaging needs. The Zebrafish heartbead was reconstructed from 2D time series at each volume plane by synchronizing the individual time series using a custom-written Python script. For more insights into the creation of this data set, read the related Image-to-Results story on the ZEISS website.

Zebrafish tail, fli1-EGFP fish line as published, DOI: 10.1006/dbio.2002.0711

All data sets were recorded during the EMBO Lightsheet course 2025 at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

^{Image courtesy: Joaquin Abugattas, Leiden University, Netherlands.}

Lattice Lightsheet 7 enables imaging of differentorgans of developing zebrafish embryos with ease. Tail, brain and even heartbeat can be imaged with subcellular resolution and high volume speeds, catering for all your imaging needs. The Zebrafish heartbead was reconstructed from 2D time series at each volume plane by synchronizing the individual time series using a custom-written Python script. For more insights into the creation of this data set, read the related Image-to-Results story on the ZEISS website.

Time Series of reconstructed Zebrafish heartbeat, fli1-EGFP fish line as published, DOI: 10.1006/dbio.2002.0711

All data sets were recorded during the EMBO Lightsheet course 2025 at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

^{Image courtesy: Vipin Balan, Polytecnico Milano, Milan, Italy.}

Lattice Lightsheet 7 enables imaging of differentorgans of developing zebrafish embryos with ease. Tail, brain and even heartbeat can be imaged with subcellular resolution and high volume speeds, catering for all your imaging needs. The Zebrafish heartbead was reconstructed from 2D time series at each volume plane by synchronizing the individual time series using a custom-written Python script. For more insights into the creation of this data set, read the related Image-to-Results story on the ZEISS website.

Zebrafish brain, fli1-EGFP fish line as published, DOI: 10.1006/dbio.2002.0711

All data sets were recorded during the EMBO Lightsheet course 2025 at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

^{Image courtesy: Li-Li Li, University of Turku, Finland.}

Lattice Lightsheet 7 enables imaging of differentorgans of developing zebrafish embryos with ease. Tail, brain and even heartbeat can be imaged with subcellular resolution and high volume speeds, catering for all your imaging needs. The Zebrafish heartbead was reconstructed from 2D time series at each volume plane by synchronizing the individual time series using a custom-written Python script. For more insights into the creation of this data set, read the related Image-to-Results story on the ZEISS website.

3D rendering of reconstructed Zebrafish heartbeat, fli1-EGFP fish line as published, DOI: 10.1006/dbio.2002.0711

All data sets were recorded during the EMBO Lightsheet course 2025 at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

^{Image courtesy: Vipin Balan, Polytecnico Milano, Milan, Italy.}

Imaging plants can be challenging due to strong autofluorescence from chlorophyll and light scattering caused by rigid cell walls and heterogeneous tissues. Lightsheet microscopy is favored for plant imaging as it reduces phototoxicity and enables deeper tissue penetration with improved sectioning. When combined with lattice light-sheet technology, this method allows for real-time observation of dynamic processes in plant cells, enhancing resolution and speed. This integration offers researchers the ability to study plant biology with greater clarity and precision.

Arabidopsis thaliana expressing GFP-tagged, chloroplast-localized protein (cyan) and choroplast autofluorescence (orange).

^{Image Courtesy: Agata Cieśla, Adam Mickiewicz University Poland. Recorded during the EMBO Lightsheet Course in Dresden.}

Imaging plants can be challenging due to strong autofluorescence from chlorophyll and light scattering caused by rigid cell walls and heterogeneous tissues. Lightsheet microscopy is favored for plant imaging as it reduces phototoxicity and enables deeper tissue penetration with improved sectioning. When combined with lattice light-sheet technology, this method allows for real-time observation of dynamic processes in plant cells, enhancing resolution and speed. This integration offers researchers the ability to study plant biology with greater clarity and precision.

Arabidopsis thaliana expressing GFP-tagged, chloroplast-localized protein (cyan) and choroplast autofluorescence (orange).

^{Image Courtesy: Agata Cieśla, Adam Mickiewicz University Poland. Recorded during the EMBO Lightsheet Course in Dresden.}

Imaging plants can be challenging due to strong autofluorescence from chlorophyll and light scattering caused by rigid cell walls and heterogeneous tissues. Lightsheet microscopy is favored for plant imaging as it reduces phototoxicity and enables deeper tissue penetration with improved sectioning. When combined with lattice light-sheet technology, this method allows for real-time observation of dynamic processes in plant cells, enhancing resolution and speed. This integration offers researchers the ability to study plant biology with greater clarity and precision.

Tobacco ER & golgi body Dynamics Dynamics of Golgi bodies (green) and endoplasmic reticulum (red) in Nicotiana tabacum leaves.

^{Image Courtesy: Dr. Verena Kriechbaumer, Oxford Brookes University, UK}