![蔡司冷冻关联工作流程 蔡司冷冻关联工作流程]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.100.100.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.360.360.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.768.768.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1024.1024.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-accessories.jpg/_jcr_content/renditions/original.image_file.1280.1280.320,0,1600,1280.file/correlative-cryo-workflow-accessories.jpg"})
蔡司冷冻关联工作流程
冷冻条件下的TEM薄片制备和体积成像
蔡司冷冻关联工作流程通过简单易用的无缝工作流程,将宽场、激光共聚焦和聚焦离子束扫描电子显微镜相关联。这一解决方案针对冷冻关联工作流程的需求对软硬件进行了优化,涵盖从荧光大分子的定位到高衬度体积成像以及冷冻透射电子断层扫描的薄片样品制备等多个方面。
接近原生状态的成像
![精简的工作流程,帮助您专注于研究 蔡司冷冻关联工作流程可让您在冷冻条件下掌握具有挑战性的不同成像模式组合。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit1.jpg/_jcr_content/renditions/original.image_file.100.50.0,24,1875,962.file/correlative-cryo-benefit1.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit1.jpg/_jcr_content/renditions/original.image_file.360.180.0,24,1875,962.file/correlative-cryo-benefit1.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit1.jpg/_jcr_content/renditions/original.image_file.768.384.0,24,1875,962.file/correlative-cryo-benefit1.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit1.jpg/_jcr_content/renditions/original.image_file.1024.512.0,24,1875,962.file/correlative-cryo-benefit1.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit1.jpg/_jcr_content/renditions/original.image_file.1280.640.0,24,1875,962.file/correlative-cryo-benefit1.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit1.jpg/_jcr_content/renditions/original.image_file.1440.720.0,24,1875,962.file/correlative-cryo-benefit1.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit1.jpg/_jcr_content/renditions/original.image_file.1875.938.0,24,1875,962.file/correlative-cryo-benefit1.jpg"})
精简的工作流程,帮助您专注于研究
蔡司冷冻关联工作流程可让您在冷冻条件下掌握具有挑战性的不同成像模式组合。这一工作流程解决方案将光学和电子显微镜连接起来,实现了冷冻体积成像和冷冻TEM薄片的高效制备。专用配件精简了工作流程,方便冷冻样品在显微镜之间的安全转移。ZEN Connect可保障数据管理,让您对整个工作流程的数据都了如指掌。一系列处理工具帮助您增强成像结果。
![双重标记酵母细胞(CNM67-tdTomato和NUP-GFP) 双重标记酵母细胞(CNM67-tdTomato和NUP-GFP)。LSM图像(左)和Crossbeam图像(右)。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.100.100.263,0,938,675.file/cryo-yeast-lsm-crossbeam.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.360.360.263,0,938,675.file/cryo-yeast-lsm-crossbeam.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.675.675.263,0,938,675.file/cryo-yeast-lsm-crossbeam.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.675.675.263,0,938,675.file/cryo-yeast-lsm-crossbeam.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.675.675.263,0,938,675.file/cryo-yeast-lsm-crossbeam.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.675.675.263,0,938,675.file/cryo-yeast-lsm-crossbeam.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.675.675.263,0,938,675.file/cryo-yeast-lsm-crossbeam.jpg"})
![双重标记酵母细胞(CNM67-tdTomato和NUP-GFP) 样品由瑞士苏黎世联邦理工学院的M. Pilhofer提供 双重标记酵母细胞(CNM67-tdTomato和NUP-GFP)。LSM图像(左)和Crossbeam图像(右)。 样品由瑞士苏黎世联邦理工学院的M. Pilhofer提供]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.100.56.file/cryo-yeast-lsm-crossbeam.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.360.203.file/cryo-yeast-lsm-crossbeam.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.768.432.file/cryo-yeast-lsm-crossbeam.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original.image_file.1024.576.file/cryo-yeast-lsm-crossbeam.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original./cryo-yeast-lsm-crossbeam.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original./cryo-yeast-lsm-crossbeam.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-yeast-lsm-crossbeam.jpg/_jcr_content/renditions/original./cryo-yeast-lsm-crossbeam.jpg"})
双重标记酵母细胞(CNM67-tdTomato和NUP-GFP)。LSM图像(左)和Crossbeam图像(右)。
出色的部件为您提供高质量的数据
借助兼容低温的冷冻物镜和Airyscan探测器的高灵敏度,蔡司LSM系统让您能够以高分辨率检测蛋白质和细胞结构,同时低光毒性的照明和恒定低温系统可防止您的样品产生去玻璃化效应。蔡司Crossbeam FIB-SEM可以让您享受高衬度的体积成像,甚至无需对您的样品进行重金属染色。这两种模式都提供了宝贵的功能和结构信息,无论您是否跟进后续的TEM研究,都可以对超微结构有全面的了解。
图片说明: 双重标记酵母细胞(CNM67-tdTomato和NUP-GFP)。LSM图像(左)和Crossbeam图像(右)。样品由瑞士苏黎世联邦理工学院的M. Pilhofer提供
![配备冷冻设备的分析测试平台 配备冷冻设备的分析测试平台]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit3.jpg/_jcr_content/renditions/original.image_file.100.50.file/correlative-cryo-benefit3.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit3.jpg/_jcr_content/renditions/original.image_file.360.180.file/correlative-cryo-benefit3.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit3.jpg/_jcr_content/renditions/original.image_file.768.384.file/correlative-cryo-benefit3.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit3.jpg/_jcr_content/renditions/original.image_file.1024.512.file/correlative-cryo-benefit3.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit3.jpg/_jcr_content/renditions/original.image_file.1280.640.file/correlative-cryo-benefit3.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit3.jpg/_jcr_content/renditions/original.image_file.1440.720.file/correlative-cryo-benefit3.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-benefit3.jpg/_jcr_content/renditions/original./correlative-cryo-benefit3.jpg"})
多用途解决方案,保证您成像设备的效率
与其他解决方案不同,工作流程中所涉及的蔡司显微镜不仅可以用于冷冻电镜,还可以用于室温应用,这在显微镜并非完全只用于冷冻实验时尤为有利。用户无需专业技术知识,即可快速将设备从冷冻切换到室温使用。该灵活性让您有更多的时间进行实验,而成像设备让您拥有更高的利用率和更快的投资回报。
蔡司冷冻关联工作流程概览
![冷冻关联工作流程 冷冻关联工作流程]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.100.67.29,0,1892,1242.file/correlative-cryo-workflow-overview.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.360.240.29,0,1892,1242.file/correlative-cryo-workflow-overview.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.768.512.29,0,1892,1242.file/correlative-cryo-workflow-overview.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.1024.683.29,0,1892,1242.file/correlative-cryo-workflow-overview.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.1280.853.29,0,1892,1242.file/correlative-cryo-workflow-overview.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.1440.960.29,0,1892,1242.file/correlative-cryo-workflow-overview.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.1863.1242.29,0,1892,1242.file/correlative-cryo-workflow-overview.png"})
![冷冻关联工作流程 冷冻关联工作流程]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.100.65.file/correlative-cryo-workflow-overview.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.360.233.file/correlative-cryo-workflow-overview.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.768.497.file/correlative-cryo-workflow-overview.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.1024.662.file/correlative-cryo-workflow-overview.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.1280.828.file/correlative-cryo-workflow-overview.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original.image_file.1440.932.file/correlative-cryo-workflow-overview.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/correlative-cryo-workflow-overview.png/_jcr_content/renditions/original./correlative-cryo-workflow-overview.png"})
解决方案概览
![蔡司冷冻配件工具包 蔡司冷冻配件工具包]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.100.56.0,0,1198,674.file/cryo-accessory-kit.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.360.203.0,0,1198,674.file/cryo-accessory-kit.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.768.432.0,0,1198,674.file/cryo-accessory-kit.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.1024.576.0,0,1198,674.file/cryo-accessory-kit.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/cryo-accessory-kit.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/cryo-accessory-kit.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.1198.674.0,0,1198,674.file/cryo-accessory-kit.jpg"})
蔡司冷冻配件工具包
![蔡司冷冻配件工具包 蔡司冷冻配件工具包]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.100.56.file/cryo-accessory-kit.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.360.203.file/cryo-accessory-kit.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.768.432.file/cryo-accessory-kit.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original.image_file.1024.576.file/cryo-accessory-kit.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original./cryo-accessory-kit.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original./cryo-accessory-kit.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-accessory-kit.jpg/_jcr_content/renditions/original./cryo-accessory-kit.jpg"})
蔡司冷冻配件工具包
蔡司冷冻配件工具包
蔡司冷冻关联工作流程可以使用各种样品载具。无论您是使用TEM铜网、AutoGrid、蓝宝石盘还是HPF板,都可以借助冷冻配件工具包轻松装载、转移和存储样品。一系列的配件和工具可保证在整个工作流程中能够安全处理样品。组件与以下设备兼容:
- Linkam CMS196V³冷冻关联显微镜载物台
- Quorum PP3010Z冷冻系统
![蔡司Crossbeam:可旋转冷冻镜台下部 蔡司Crossbeam:可旋转冷冻镜台下部]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.100.75.240,0,1680,1080.file/cryo-crossbeam-substage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.360.270.240,0,1680,1080.file/cryo-crossbeam-substage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.768.576.240,0,1680,1080.file/cryo-crossbeam-substage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.1024.768.240,0,1680,1080.file/cryo-crossbeam-substage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.1280.960.240,0,1680,1080.file/cryo-crossbeam-substage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.1440.1080.240,0,1680,1080.file/cryo-crossbeam-substage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.1440.1080.240,0,1680,1080.file/cryo-crossbeam-substage.jpg"})
蔡司Crossbeam:可旋转冷冻镜台下部
![蔡司Crossbeam:可旋转冷冻镜台下部 蔡司Crossbeam:可旋转冷冻镜台下部]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.100.56.file/cryo-crossbeam-substage.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.360.203.file/cryo-crossbeam-substage.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.768.432.file/cryo-crossbeam-substage.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.1024.576.file/cryo-crossbeam-substage.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.1280.720.file/cryo-crossbeam-substage.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original.image_file.1440.810.file/cryo-crossbeam-substage.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/cryo-crossbeam-substage.jpg/_jcr_content/renditions/original./cryo-crossbeam-substage.jpg"})
蔡司Crossbeam:可旋转冷冻镜台下部
蔡司Crossbeam安全易操作的样品转移和处理
蔡司冷冻关联工作流程配有Quorum PP3010Z,一个高度自动化、简单易用的气冷式冷冻制备系统。
- 冷冻制备室使用了涡轮分子泵,包括可控自动升华和喷镀的工具。
- 玻璃化样品从冷冻制备室直接连接到蔡司Crossbeam样品仓,在一个高度稳定的冷载物台上进行成像和切割。
- 冷冻制备室和Crossbeam样品仓中的冷阱可以保护样品免受冰污染。
- CHE3010柱外冷却系统确保了至少24小时的连续冷却。
- 所有Quorum冷冻组件均由Prepdek®工作站控制,该工作站包含了用于冷冻转移设备的真空存储管以及用于蔡司装载站的TEM prep slusher。
![蔡司ZEN Connect中的冷冻关联数据集 蔡司ZEN Connect中的冷冻关联数据集]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.100.56.file/zen-connect-cryo-correlation.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.360.203.file/zen-connect-cryo-correlation.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.768.432.file/zen-connect-cryo-correlation.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.1024.576.file/zen-connect-cryo-correlation.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.1280.720.file/zen-connect-cryo-correlation.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.1440.810.file/zen-connect-cryo-correlation.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original./zen-connect-cryo-correlation.jpg"})
蔡司ZEN Connect中的冷冻关联数据集
![蔡司ZEN Connect中的冷冻关联数据集 蔡司ZEN Connect中的冷冻关联数据集]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.100.56.file/zen-connect-cryo-correlation.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.360.203.file/zen-connect-cryo-correlation.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.768.432.file/zen-connect-cryo-correlation.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.1024.576.file/zen-connect-cryo-correlation.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.1280.720.file/zen-connect-cryo-correlation.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original.image_file.1440.810.file/zen-connect-cryo-correlation.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/zen-connect-cryo-correlation.jpg/_jcr_content/renditions/original./zen-connect-cryo-correlation.jpg"})
蔡司ZEN Connect中的冷冻关联数据集
一站式应用:配置完备的软件包
为了确保冷冻关联工作流程的简洁高效以及各种组件的无缝配合,我们扩展了相关软件平台,添加了冷冻特定功能。为应对关联冷冻电镜带来的挑战,我们还开发了额外的软件模块。
- ZEN
- ZEN Connect Toolkit
- ZEN EM处理工具箱
- SmartSEM和SmartFIB
- 冷冻漂移矫正
![纵向切开的核膜内的纺锤极体(上图)和横向切开的核膜外的微管(下图)。 纵向切开的核膜内的纺锤极体(上图)和横向切开的核膜外的微管(下图)。获取序列图像的图像步长:50 nm]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.100.25.0,65,1480,435.file/mtoc-series.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.360.90.0,65,1480,435.file/mtoc-series.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.768.192.0,65,1480,435.file/mtoc-series.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.1024.256.0,65,1480,435.file/mtoc-series.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.1280.320.0,65,1480,435.file/mtoc-series.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.1440.360.0,65,1480,435.file/mtoc-series.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.1480.370.0,65,1480,435.file/mtoc-series.jpg"})
![纵向切开的核膜内的纺锤极体(上图)和横向切开的核膜外的微管(下图)。 纵向切开的核膜内的纺锤极体(上图)和横向切开的核膜外的微管(下图)。获取序列图像的图像步长:50 nm]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.100.34.file/mtoc-series.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.360.122.file/mtoc-series.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.768.259.file/mtoc-series.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.1024.346.file/mtoc-series.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.1280.432.file/mtoc-series.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original.image_file.1440.486.file/mtoc-series.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/products/imaging/correlative-cryo/applications/mtoc-series.jpg/_jcr_content/renditions/original./mtoc-series.jpg"})
纵向切开的核膜内的纺锤极体(上图)和横向切开的核膜外的微管(下图)。获取序列图像的图像步长:50 nm
纵向切开的核膜内的纺锤极体(上图)和横向切开的核膜外的微管(下图)。获取序列图像的图像步长:50 nm