A Ten-color Spectral Imaging Strategy to Reveal Localization of Gut Immune Cell Subsets
Explore the ZEISS options for advanced multiplexing applications including the new Axioscan 7 and NIR for the LSM 980. Let our ZEN software guide you in quickly going from placing your sample to getting quantitative data.
Expand Your Spectral Range
Expanding your spectral range into the NIR allows you to use more labels in parallel. Visualize additional structures with more dyes in your multi-color experiments, with the Quasar and NIR detectors efficiently supporting spectral multiplexing experiments. NIR fluorescent labels are less phototoxic for living samples due to the longer wavelength. This allows you to investigate living samples for longer periods of time while limiting the influence of light. Additionally, light of longer wavelength ranges is less scattered by the sample tissue, increasing penetration depth.
For any of the advantages you pursue with NIR labels, the dual-channel NIR detector combines two different detector technologies (extended red GaAsP and GaAs) for optimal sensitivity up to 900 nm.
LSM 980 brings you the best of two worlds to image your most challenging samples. You get the light efficient beam path of the LSM 9 family with up to 36 simultaneous channels for full spectral flexibility up to the near infrared (NIR) range. Plus, when you combine it with Airyscan 2, this revolutionary area detector extracts even more information from your sample in less time. This lets you image faint signals with 4 – 8 times higher signal-to-noise ratio (SNR). You don't need to close a pinhole to get super-resolution, which makes your 3D imaging even more light efficient.
It's never been easier to set up complex confocal live cell imaging experiments. ZEN microscopy software puts a wealth of helpers at your command to achieve reproducible results in the shortest possible time.
AI Sample Finder helps you quickly find regions of interest, leaving more time for experiments. Smart Setup supports you in applying best imaging settings for your fluorescent labels. Direct Processing enables parallel acquisition and data processing. ZEN Connect keeps you on top of everything, both during imaging and later when sharing the whole story of your experiment.
Speed, gentle treatment and the optimal wavelength are critical when it comes to multispectral fluorescence imaging. Axioscan 7 employs swift and reproducible LED illumination, fast filter wheels, and a sophisticated filter concept to efficiently separate a broad range of fluorescence channels.
Let Smart Setup guide you through the experiment design and watch AI Sample Finder automatically find your sample region. Even with low-contrast samples, you will quickly obtain an overview image to access relevant regions with just a click. Reduce the time to image from minutes to just seconds and start your experiment right away. Use ZEN Connect to catalog, correlate, and navigate, keeping it all together even across different microscopes.
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