Multiplex Imaging Solutions

Expand your Spatial Biology applications with the suite of ZEISS Multiplex Imaging and Software solutions

Explore the ZEISS options for advanced multiplexing applications including the new Axioscan 7 and NIR for the LSM 980. Let our ZEN software guide you in quickly going from placing your sample to getting quantitative data.


Your Unique Confocal Experience for Fast and Gentle Multiplex Imaging

  • Typical spectral quantum efficiency (QE) of ZEISS LSM 980 detectors, including NIR.

    Typical spectral quantum efficiency (QE) of ZEISS LSM 980 detectors, including NIR.

Near-infrared Imaging Allows You to Conduct New Experiments

Expand Your Spectral Range

Expanding your spectral range into the NIR allows you to use more labels in parallel. Visualize additional structures with more dyes in your multi-color experiments, with the Quasar and NIR detectors efficiently supporting spectral multiplexing experiments. NIR fluorescent labels are less phototoxic for living samples due to the longer wavelength. This allows you to investigate living samples for longer periods of time while limiting the influence of light. Additionally, light of longer wavelength ranges is less scattered by the sample tissue, increasing penetration depth.

For any of the advantages you pursue with NIR labels, the dual-channel NIR detector combines two different detector technologies (extended red GaAsP and GaAs) for optimal sensitivity up to 900 nm.

  • Increased Spectral Range
    Unravel the complex interactions in spatial biology.
  • Increased Depth Penetration
    Increase your depth information with emerging NIR dye technology for live animal imaging.
  • Less Phototoxicity
    Image your samples more gently and/or for longer periods of time.

Learn how to do multiplex imaging on the ZEISS LSM

Application Note

A Ten-color Spectral Imaging Strategy to Reveal Localization of Gut Immune Cell Subsets

ZEISS Axioscan 7

for your Slide Scanning Multiplexing Experiments

Highly Productive Fluorescence Imaging

Speed, gentle treatment and the optimal wavelength are critical when it comes to multispectral fluorescence imaging. Axioscan 7 employs swift and reproducible LED illumination, fast filter wheels, and a sophisticated filter concept to efficiently separate a broad range of fluorescence channels.

  • Shortest possible exposure times for maximum specimen protection
  • Fast switching between up to 9 fluorescence channels
  • Perfect spectral separation for advanced fluorescence multiplexing applications
  • Unparalleled information density without compromising data quality

Learn more about ZEISS Axioscan 7

Watch the webinar

Key Learnings:  

  • The main drivers that push researchers to move to an automated platform
  • How Axioscan 7 makes routine imaging more efficient
  • Example applications of multiplex imaging in the mouse brain

Widefield Solutions for Multiplex Imaging

Speed up your time to imaging and automate your results using AI technology

Take the Work out of Imaging

Let  Smart Setup  guide you through the experiment design and watch  AI Sample Finder  automatically find your sample region. Even with low-contrast samples, you will quickly obtain an overview image to access relevant regions with just a click. Reduce the time to image from minutes to just seconds and start your experiment right away. Use  ZEN Connect  to catalog, correlate, and navigate, keeping it all together even across different microscopes.

AI Sample Finder

  • Detect, Calibrate, Focus and Find
  • Hands-free, damage-free and trouble-free
  • Tissue regions are automatically detected and imported into experiment setup

ZEN Connect

  • Catalog images automatically within a single project
  • Correlate samples across different microscope modalities WF to Super Res to EM
  • Navigate within your images to drive the stage for further acquisition

Learn more about AI Sample Finder

Watch the virtual demonstration

Key Learnings:  

  • Rapidly generate a navigable overview image with assigned sample regions with minimal user interaction
  • Use deep-learning algorithms for automatic calibration of carriers, such as well plates
  • Capture fast, detailed overviews using a novel contrasting technique

Colibri 7

Flexible and Super-Fast

Multicolor LED Light Source for Gentle Fluorescence Imaging

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