Application Note
A Ten-color Spectral Imaging Strategy to Reveal Localization of Gut Immune Cell Subsets
Explore the ZEISS options for advanced multiplexing applications including the new Axioscan 7 and NIR for the LSM 980. Let our ZEN software guide you in quickly going from placing your sample to getting quantitative data.
Expand Your Spectral Range
Expanding your spectral range into the NIR allows you to use more labels in parallel. Visualize additional structures with more dyes in your multi-color experiments, with the Quasar and NIR detectors efficiently supporting spectral multiplexing experiments. NIR fluorescent labels are less phototoxic for living samples due to the longer wavelength. This allows you to investigate living samples for longer periods of time while limiting the influence of light. Additionally, light of longer wavelength ranges is less scattered by the sample tissue, increasing penetration depth.
For any of the advantages you pursue with NIR labels, the dual-channel NIR detector combines two different detector technologies (extended red GaAsP and GaAs) for optimal sensitivity up to 900 nm.
Speed, gentle treatment and the optimal wavelength are critical when it comes to multispectral fluorescence imaging. Axioscan 7 employs swift and reproducible LED illumination, fast filter wheels, and a sophisticated filter concept to efficiently separate a broad range of fluorescence channels.
Let Smart Setup guide you through the experiment design and watch AI Sample Finder automatically find your sample region. Even with low-contrast samples, you will quickly obtain an overview image to access relevant regions with just a click. Reduce the time to image from minutes to just seconds and start your experiment right away. Use ZEN Connect to catalog, correlate, and navigate, keeping it all together even across different microscopes.
Please fill out the form below to get in contact with a ZEISS representative about the specific needs of your facility.