Light Sources for Fluorescence Imaging
Choose between Colibri 7, the super-fast 7 wavelengths LED light source from ZEISS, or the white light LED light source X-Cite Xylis:
Digitize your specimens with Axioscan 7 – the reliable, reproducible way to create high-quality virtual microscope slides. Axioscan 7 combines qualities that you would not expect to get in a slide scanner: high speed digitization and outstanding image quality plus an unrivaled variety of imaging modes are all available in a fully automated and easy to operate system.
From an automated system, you expect absolute reliability for continuous operation. ZEISS Axioscan 7 repeatedly produces digitized slides at dramatically improved speed, thanks to hardware components designed for extended, uninterrupted operation. A fully motorized condenser, powerful light sources and sensitive cameras ensure 24/7 scan performance, whether you have many similar slides or mixed applications to process. Easily assigned scan profiles allow acquisition runs to be set up quickly. Axioscan 7 software is built to flawlessly process large amounts of raw data – in the range of several terabytes.
ZEISS Axioscan 7 allows rapid switching among fluorescence, brightfield and polarization scanning modes, with the highest quality fast and gentle imaging fully available to you in each of these modes. Benefit from contrast flexibility and high scan speed when digitizing HE-stained tissue samples or other brightfield applications. Fast filter wheels and a spectral range up to far red excitation light expand your fluorescence imaging capabilities – in combination with the new sample-preserving contrast method Transfer of Intensity Equation, multiplex imaging reaches a new level of performance. The combination of all imaging modes let you extract maximum sample information with a minimum of effort.
ZEN Slidescan is integrated within the powerful ZEN imaging software universe, which provides access to numerous additional processing and analysis functions. ZEN Connect, the ZEISS software for correlative microscopy, enables more advanced workflows – from automated slide scanning to detailed studies on other microscope systems. The established CZI data format opens the possibility to use additional third-party data analysis tools. With ZEN Data Storage and ZEN Data Explorer, you can access and share your scanned data from anywhere, at any time.
Caption: Automated slide scanning and detailed studies on other ZEISS microscope systems combined in a ZEN Connect project
Speed, gentle treatment and the optimal wavelength are critical when it comes to multispectral fluorescence imaging. Axioscan 7 employs swift and reproducible LED illumination, fast filter wheels, and a sophisticated filter concept to efficiently separate a broad range of fluorescence channels.
The newly designed condenser with its motorized modulator disk allows automatic switching between different brightfield imaging modes to adapt to the different requirements of your applications, opening a new range of experiments and modality combinations.
The Axioscan 7 brightfield imaging performance is driven by a motorized condenser and a powerful white light source
Colibri 7 operates with reproducible output power levels for each wavelength and therefore produces consistent quantitative data for all important dyes, fluorescent proteins and probes. Individual LEDs and integrated excitation filters make an additional filter wheel unnecessary and allow switching times of a few milliseconds between the color channels.
The use of X-Cite Xylis together with a fast excitation filter wheel enables long wavelength sample illumination up to 770 nm. In addition, the green gap – typically a problem with LED fluorescent light sources – is overcome and comparable to classic arc lamps in this spectral range.