Focus on Microscopy 2023

Speaker: Klaus Weisshart

Time and Location: Monday, April 3, 14.20 – 14.40 pm, Infante Hall

Abstract:
Structured Illumination Microscopy (SIM) is a well-established method not only for optical sectioning but also super-resolution microscopy. In the classical SIM approach a 1-dimensional grid such as a line grating is used for structuring. Over the last years various 2-dimensional patterns have been employed leading among others to improved penetration depth.
We will present how innovation on the encoding and decoding parts of structed illumination opens the possibility to distribute the photon budget according to the need of the experiment. With the example of ELYRA 7 we present data how reduced numbers of required raw images adapts the system perfectly to the special needs of capturing fast dynamics in living cells like for example organelle interactions. Moreover, sample-dependent resolution well below 100 nm can be achieved.

Speaker: Chris Power

Time and Location: Tuesday, April 4, 16.10 – 16.30 pm, Archive Hall

Abstract:
Learn how to overcome the typical imaging and analysis challenges in neuroscience research, with the ZEISS LSM 9 series and arivis. LSM Plus and Airyscan joint Deconvolution (jDCV) can enable you to reliably gather higher quality data from your confocal imaging, enhancing resolution and signal to noise (SNR). Explore how the automatic end-to-end pipelines and AI-driven image analysis by arivis allow you to push the boundaries of your research. Automatic Neuron Tracing allows you to adapt the methods and parameters to your specific microscopy modalities and datasets, thus helping you to get the most out of your valuable imaging data from every sample.

Speaker: Kirstin Elgass

Time and Location: Monday, April 3, 17:50-19:20 pm, Poster Hall

Topic:
Light sheet fluorescence microscopy has become an established method for fast and unsurpassed gentle imaging of living specimens. By adding lattice structures to the light sheet, ZEISS Lattice Lightsheet 7 makes this technique available for live cell imaging at subcellular resolution. The lattice light-sheet can be adapted to each experiment enabling visualization of previously unobserved events in the most challenging of samples, such as a single cell undergoing multiple mitotic waves, stem cell organoid development, malaria parasite invasion, and T-cell attacks.
We will present technical details on the innovative approaches that turn Lattice Lightsheet 7 into an easy-to-use turn-key system leading to unique applications and research outcomes that demonstrate the potential for Lattice Lightsheet technology to drive new discoveries in biological and medical research.