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Laser Scanning Microscopy

Advanced F-Techniques

FRAP & FRET

Advanced fluorescence techniques, commonly known as the F-techniques, measure the kinetics and the interactions of biomolecules with high sensitivity and spatiotemporal resolution.

Course Details

Description

This course provides detailed theoretical and practical knowledge about principles and applications of the most commonly used F-techniques: Fluorescence Recovery After Photo-bleaching (FRAP) & Förster Resonance Energy Transfer (FRET) on the LSM 9 family.

Topics of the course
  • FRAP (Fluorescence Recovery After Photobleaching)
  • FRET (Forster Resonance Energy Transfer), a technology to prove molecular interaction on the basis of (confocal) light microscopy
  • Theory of other Photomanipulation strategies (e.g. Photoconversion)

Learning Level

Advanced

Prerequisites

Fundamental knowledge about confocal laser scanning microscopy
ZEN Modules FRAP and FRET are recommended

Systems

LSM 9 family

Software

ZEN (blue edition)

Date

December 13 to 14, 2023
Registration until 2 weeks prior to the course

Duration

2 days (2 x 4 hours)
Course takes place in the European timezone (CET/CEST)

Location

Virtual classroom
(Required: reliable internet connection; computer speakers, headphones or a reliable telephone connection; Microsoft Teams and/or TeamViewer)

Language

English

Course Fee

Per person: 945 € (plus VAT)

Participants

Max. 3

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