Laser Scanning Microscopy

Fluorescence Correlation Spectroscopy (FCS)

@ LSM 980

Fluorescence Correlation Spectroscopy (FCS) is a highly sensitive method that detects movement and binding of fluorescence-labeled particles on the single molecule level. Furthermore, concentrations of movable fluorescent particles can be estimated. Thus biochemical reactions can be monitored in real time solutions as well as within living cells.

Course Details

Description

This course will familiarize you with the theoretical background of FCS and train you on how to perform and evaluate measurements in solutions.

Topics of the course

  • Theory of FCS
  • Correlation function
  • Model functions for diffusion processes
  • Data acquisition for FCS and FCCS
  • Data analysis for FCS and FCCS
  • Objective lenses for FCS and FCCS
  • Short introduction to RICS

Learning Level

Expert

Prerequisites

Profound knowledge of LSM 980 is recommended

Systems

LSM 980

Software

ZEN (blue edition)

Date

March 1 to 2, 2023
Registration until 2 weeks prior to the course

Duration

2 days

Location

Training and Support Center Jena

Language

English

Course Fee

Per person: 1440 € (plus VAT)

Participants

Max. 3

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