
ZEISS Primo Star iLED Observe microbiological structures
Primo Star iLED is your recommended microscope to visualize structures smaller than 0.5 µm with LED fluorescence excitation and transmitted-light brightfield illumination such as the rod-shaped bacterium Mycobacterium tuberculosis. Mycobacterium tuberculosis comes with a size of 2 – 4 µm in lengths and 0.2 – 0.5 µm in width.
Based on experiences in the WHO Supranational Reference Laboratory of Tuberculosis, Gauting, Germany, tuberculosis may whether analyzed with Ziehl-Neelsen-staining or fluorescence excitation, e.g. Auramine O.

Stop TB Partnership
Primo Star iLED is the result of a cooperation between ZEISS and the Foundation for Innovative New Diagnostics (FIND). Through the targeted promotion of diagnostic innovations, the Swiss foundation FIND has set itself the objective of combating the spread of diseases. Therefore, Primo Star iLED is available for you at a particularly low price.
Auramine O staining
In this video tutorial Dr Harald Hoffmann, MD and head of the WHO-certified supranational reference laboratory IML in Gauting, Germany, demonstrates how to stain smear preparations for diagnosis of tuberculosis with fluorescence microscopy.
*The protocol and microscope recommendation shown in the video is based on the experience of the WHO reference laboratory in Gauting.
Application Examples

Representative example of tubercle bacilli in brightfield illumination

Representative example of tubercle bacilli in brightfield illumination

Representative example of tubercle bacilli in fluorescence illumination
