Frequently Asked Questions About Imaging Cleared Tissues

17 June 2020 · 20 min watch
  • Life Sciences
  • Light Sheet Microscopy
Author Courtney Akitake Ph.D Product Marketing Manager - Lightsheet
ZEISS Microscopy

Frequently Asked Questions About Imaging Cleared Tissues

This presentation addresses five frequently asked questions that typically arise while preparing and imaging optically cleared tissue samples with Lightsheet and other microscopes:

  1. Why do I need to clear my sample?
  2. What clearing method should I use?
  3. How do I image my sample after clearing?
  4. How can I improve my image quality?
  5. How do I manage all the data?

The information presented here is intended to serve as an introductory overview for researchers that are just getting into tissue clearing, as well as a reference for more advanced individuals that want to optimize imaging of their already cleared samples.

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