A New Confocal Experience

Learn more about LSM Plus and Airyscan jDCV

Laser scanning microscopy is valued for its instant, high-quality imaging of optical sections and has set the imaging standard for a wide variety of samples and experiments. It’s hard to imagine how the data quality of this technology can be improved further while fully preserving its appreciated ease of use and application flexibility.

The ZEISS LSM product family, now featuring LSM Plus and Airyscan Joint Deconvolution (jDCV), is doing just that: improving literally any confocal experiment with ease, independent of detection mode or emission range.

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March 1, 2022

Join us on March 1, 2022 for a virtual live user workshop and learn more about LSM Plus and Airyscan jDCV, see how they are used, and ask your questions.

LSM Plus

Improve spectral imaging along the complete wavelength range

This new linear Wiener filter deconvolution needs next to no interaction while still ensuring a reliable quantitative result, regardless of whether you perform classic confocal or advanced multispectral imaging including NLO and NIR. Just as in the time-tested Airyscan super-resolution processing, the underlying optical property information is adapted automatically based on objective lens, refractive index, and emission range.

Apply LSM Plus with no extra effort and benefit from:

  • Enhanced signal to noise at high acquisition speed and low laser power—particularly useful for live cell imaging with low expression levels
  • Improved resolution of spectral data with up to 36 channels in a single scan
  • More spatial information and even greater resolution enhancement for bright samples that allow to close the pinhole of the LSM
  • Integrated workflows to combine the advantages of LSM Plus with Airyscan super-resolution imaging
Cos-7 cells Anti-TOM20 AF750 (red), Anti-Tubulin AF700 (cyan), Actin Phalloidin-OG488 (magenta), DAPI (orange).
Cos-7 cells Anti-TOM20 AF750 (red), Anti-Tubulin AF700 (cyan), Actin Phalloidin-OG488 (magenta), DAPI (orange). Sample: courtesy of U. Ziegler and J. Doehner, University of Zurich, ZMB, Switzerland.
Separated fluorescent signals. The comparison illustrates how LSM Plus improves SNR and resolution.
Separated fluorescent signals. The comparison illustrates how LSM Plus improves SNR and resolution. Sample: courtesy of U. Ziegler and J. Doehner, University of Zurich, ZMB, Switzerland.
RPE1 Cells Transfected with H2B-GFP Plasmid
RPE1 cells transfected with H2B-GFP plasmid. Maximum intensity pr ojection of 117 z planes. Comparison of without (left) and with LSM Plus (right). Courtesy of Tingsheng Liu, Mitosis Lab, Singapore.
Live Imaging of LLC-PK1 Dividing Cell (Porcine Kidney)
Live imaging of LLC-PK1 dividing cell (porcine kidney), expressing H2B-mCherry (red) and α-Tubulin-mEGFP (cyan). Maximum intensity projection of 37 z-planes. Comparing without (left) and with LSM Plus (right).

Airyscan Joint Deconvolution

Add structural information and push resolution even further

Airyscan 2 is an area detector with 32 circularly arranged detection elements. Each of these acts as a small pinhole, contributing to super-resolution information, while the complete detector area collects more light than the standard confocal setting. This produces much greater light efficiency while capturing enhanced structural information.

32 Views Mean More Information

Each of the 32 detector elements has a slightly different view on the sample, providing additional spatial information that makes Joint Deconvolution possible. This reduces the distance that can be resolved between two points even further—down to 90 nm. Your super-resolution experiments will benefit from an improved separation of single or multiple labels.

Mitochondria in an Arabidopsis Thaliana Cell
Comparing the confocal image (left) with Airyscan SR (middle) and Airyscan Joint Deconvolution (right). Mitochondria in an Arabidopsis thaliana cell. mCherry (green) is targeted to the matrix and GFP (magenta) to the intermembrane space. Courtesy of J.-O. Niemeier, AG Schwarzländer, WWU Münster, Germany.
HeLa Cell - 4× Expanded and Labelled with Acetylated Alpha Tubulin (green)
Comparing the confocal image (left) with Airyscan SR (middle) and Airyscan Joint Deconvolution (right). HeLa cell, 4× expanded and labelled with acetylated alpha tubulin (green). Courtesy of S. Zhang, Prof. Liou Yih-Cherng's lab, Singapore.
GATTA SIM Nanoruler
GATTA SIM nanoruler imaged with Airyscan SR (GATTA-SIM 120B, left) and Airyscan jDCV (GATTA-SIM 90B, right).
Spermatogonia in Drosophila Testis
Spermatogonia in Drosophila testis. Multi-color label with asterless (magenta), acetylated tubulin (cyan), and Hoechst 33258 (yellow). Imaged with ZEISS Airyscan 2 followed by Joint Deconvolution. Courtesy of S. Song, Prof. Liou Yih-Cherng's lab, Singapore.

Learn More about the Technology

In these two white papers, read more about the technology behind LSM Plus and Airyscan Joint Deconvolution (jDCV).

A Practical Guide of Deconvolution

Application Note

A Practical Guide of Deconvolution - Application Note

The Basic Principle of Airyscanning

Technology Note

The Basic Principle of Airyscanning - Technology Note

Get the New Confocal Experience

ZEISS LSM Plus and Airyscan Joint Deconvolution (jDCV) are available as upgrades for the following systems:

  LSM Plus Airyscan jDCV

LSM 800
LSM 800 with Airyscan

LSM 880
LSM 880 with Airyscan

-

✔ 
on additional processing PC

LSM 900
LSM 900 with Airyscan 2

LSM 980
LSM 980 with Airyscan 2

Celldiscoverer 7 with LSM 900
Celldiscoverer 7 with LSM 900 Airyscan 2

Your compact confocal for gentle multiplex imaging and smart analysis.

Your unique confocal experience for fast and gentle multiplex imaging.

Get in Contact with Us

Do you have questions about ZEISS LSM Plus or Airyscan jDCV or want to upgrade? Get in contact with one of our confocal imaging specialists.
We look forward to hearing from you!

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