ZEISS Celldiscoverer 7
Product

ZEISS Celldiscoverer 7

Your Automated Microscope for Live Cell Imaging

Combine the ease of use of an automated microscope with the image quality and flexibility of a research microscope. Whether working with 2D or 3D cell cultures, tissue or small model organisms, you will acquire better data in shorter times with this automated live cell imaging platform. Add LSM 900 with Airyscan 2 to gently image dynamic processes with highest framerates in superresolution.

  • Automated high-end live cell imaging
  • Calibration routines for reproducible results
  • Automatic sample recognition and barcode reading
  • Fast and gentle multi-fluorescence imaging
  • High-quality 3D data with LSM 900 and Airyscan 2
A Flexible Platform For Demanding Applications

A Flexible Platform

For Demanding Applications

The fully integrated high-end imaging system comes with various incubation and detection options so you can tailor it to your applications. Go for fast, sensitive cameras when performing demanding live cell experiments and rapid time-lapse recordings. To get better data from your 3D samples, simply add LSM 900 with Airyscan 2 for confocal super-resolution imaging, or fast GPU-based deconvolution.

Top Quality Data

From Living Samples

For long-term, time-lapse imaging, Celldiscoverer 7 gives you the advantage of Autoimmersion and a focus that is kept automatically after detecting the thickness and optical properties of the sample carrier. Autocorr objectives correct spherical aberrations to deliver crisp contrast and high resolution. Integrated incubation options keep your cells happy to deliver unbiased data.

Reproducible Results Made Easy

Reproducible Results

Made Easy

As soon as you start imaging, automatic calibration routines take over to ensure reproducible results. Check the current status and follow progress of your experiments on the touchscreen. With barcode recognition you can identify your sample, sample carrier and even the type of experiment. If you don’t work with barcodes, an automatic preview scan will identify the sample carrier and calibrate it.

ZEISS Celldiscoverer 7 Product Family

ZEISS Celldiscoverer 7 (Widefield)
Celldiscoverer 7 (Widefield)
ZEISS Celldiscoverer 7 with LSM 900 (Confocal)
Celldiscoverer 7 with LSM 900 (Confocal)

Imaging Modes

Widefield imaging

Confocal imaging with LSM 900

 

LSM Plus

 

optional

Airyscan 2

 

optional

Airyscan 2 Multiplex

 

optional

Airyscan jDCV

optional

Automated Workflows and Advanced Methods

Guided Acquisition

BioApps

ZEN Intellesis – AI based image segmentation

ZEN Connect – connect all multi-modal data

(Automated) Photomanipulation

 

Optical sectioning

 

Simultaneous widefield and confocal imaging

 

External Cameras

Axiocam 712 mono

optional

Axiocam 702 mono

optional

 

CMOS camera Prime 95B

optional

 

Hamamatsu Orca Flash 4.0 V3

optional

 

ZEISS Celldiscoverer 7 Technology Insights

A Wealth of Options to Automate Your Live Cell Imaging

Spherical aberration due to unadjusted optics. Tubulin in FluoCells prepared slide #1.
Same structure using an Autocorr objective. Tubulin in FluoCells prepared slide #1.
Left: spherical aberration due to unadjusted optics. Right: same structure using an Autocorr objective. Tubulin in FluoCells prepared slide #1. Sample courtesy of Invitrogen, Thermo Fisher Scientific Inc.

Automatic Adaptation to Your Samples and Sample Carriers

Live cell imaging requires objectives with high numerical apertures. Those objectives will only deliver high contrast and sensitivity if their optics can adapt to variations in bottom thickness or to the material of different sample carriers. You are free to use Petri dishes, chamber slides, multi-well plates, plastic or glass, thin or thick vessel bottoms, low-skirt or high-skirt plates. Automatic sample recognition detects all relevant vessel features while loading your sample. Autocorr adjusts the correction ring of the objective to compensate for spherical aberrations.

ZEISS Celldiscoverer 7 water immersion objective

ZEISS Celldiscoverer 7 water immersion objective

Autoimmersion: There Is No Life Without Water

In life sciences, cell biology or screening applications, your samples mostly consist of water and / or will be mounted in aqueous solutions. Celldiscoverer 7 combines an outstanding 50x water immersion objective with rapid automated immersion supply and removal. An elastic silicon membrane simultaneously seals the sample chamber to avoid unnecessary airflow while protecting the system from potential liquid spillage. You can perform unbiased live cell experiments at 37 °C over several days or carry out extensive scanning processes on multi-well plates.

ZEISS Celldiscoverer 7 - Find Focus function

Find Focus – Keep Focus

Use the hardware-based Find Focus function to automatically focus your sample and find your region of interest quickly with just a single click. Select Definite Focus to maintain the focal position throughout your live cell experiment, whether it takes a few seconds or several days. Or combine both methods: Celldiscoverer 7 can automatically create focus maps for multiple positions in long-term time-lapse experiments.

ZEISS Celldiscoverer 7 - LED Technology for Live Cell Imaging

LED Technology for Live Cell Imaging

Use the advantages of LED-technology for efficient illumination with low phototoxicity, fast switching times and long-term stability. That’s what delivers gentle live cell imaging, increased throughput and reproducible results. The fluorescence excitation unit combines up to seven LEDs for maximum flexibility in the choice of dyes – from deep blue to far-red. Celldiscoverer 7 simply switches LEDs on / off – without moving any mechanical parts – so you get high-speed multi-channel imaging, even when combined with transmitted light.

Hoechst – Chromatin (blue), anti-alpha-tubulin antibody FITC for alphas tubulin (green), Phalloidine for actin (red), MitoTracker Deep Red for mitochondria (purple).
Hoechst – Chromatin (blue), anti-alpha-tubulin antibody FITC for alphas tubulin (green), Phalloidine for actin (red), MitoTracker Deep Red for mitochondria (purple). Sample courtesy of P. Denner, Core Research Facilities, German Center of Neuro-degenerative Diseases (DZNE), Bonn, Germany.
Sample courtesy of P. Denner, Core Research Facilities, German Center of Neuro-degenerative Diseases (DZNE), Bonn, Germany.

SH-SY5Y cells cultured on a 384 micro-well plate; multichannel image at a single position. Hoechst – Chromatin (blue), anti-alpha-tubulin antibody FITC for alphas tubulin (green), Phalloidine for actin (red), MitoTracker Deep Red for mitochondria (purple). 

SH-SY5Y cells cultured on a 384 micro-well plate; multichannel image at a single position. Hoechst – Chromatin (blue), anti-alpha-tubulin antibody FITC for alphas tubulin (green), Phalloidine for actin (red), MitoTracker Deep Red for mitochondria (purple). Sample courtesy of P. Denner, Core Research Facilities, German Center of Neuro-degenerative Diseases (DZNE), Bonn, Germany.

LSM 900 beampath

LSM 900 with Airyscan 2

Automated Confocal 3D Imaging

Life happens in 3D. Add LSM 900 with Airyscan 2 and get the best of both worlds: ease of use and automation from a fully integrated microscope platform and the superb confocal image quality and flexibility of LSM 9 family. Super-resolution 3D imaging with up to 1.9x resolution improvement becomes possible. Easily separate multiple labels with spectral imaging. Analyze dynamic processes in living samples with photomanipulation for FRAP, FRET or related techniques. Precisely connect widefield and confocal images. Fast mixed-mode acquisition simplifies and speeds up your workflow and gives you unique insights into your sample.

ZEISS Celldiscoverer 7 at Work

  • 384 microwell plate imaged with different magnifications in 3 channels.
  • HeLa Kyoto cell growth assay imaged every 15 minutes for 72 hours using Autoimmersion.
  • Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo. Overview image: Phase Gradient Contrast with fluorescence acquisition. High-resolution images: Airyscan 2 Multiplex.
  • Rat cortical primary neuron culture. Deconvolved images using GPU-based deconvolution.
  • Expansion microscopy in mouse brain. Left: whole brain; top right: axon bundles, bottom right: pyramidal cells.
  • Growth of cultured cells imaged over 72 hours using phase gradient contrast. To quantify proliferation, cell region was detected using machine learning. The growth curve shows relative cell coverage over time
  • Living cricket embryos mounted in low-melt agarose, expressing nuclear-localized GFP, multi-position experiment.

    Growth of cultured cells imaged over 72 hours using phase gradient contrast. To quantify proliferation, cell region was detected using machine learning. The growth curve shows relative cell coverage over time. Sample and assay courtesy of P. Denner, Core Research Facilities, German Center of Neurodegenerative Diseases (DZNE), Bonn, Germany.

  • Starlet sea anemone (Nematostella vectensis) imaged with Airyscan 2 high sensitivity mode.

    Starlet sea anemone (Nematostella vectensis) imaged with Airyscan 2 high sensitivity mode. Sample courtesy of A. Stokkermans, Ikmi Group, EMBL, Heidelberg, Germany.

  • 384 microwell plate imaged with different magnifications in 3 channels.

    384 microwell plate imaged with different magnifications in 3 channels. Sample courtesy of P. Denner, Core Research Facilities, German Center of Neurodegenerative Diseases (DZNE), Bonn, Germany.

  • HeLa Kyoto cell growth assay imaged every 15 minutes for 72 hours using Autoimmersion.

    HeLa Kyoto cell growth assay imaged every 15 minutes for 72 hours using Autoimmersion. Sample courtesy of I. Charapitsa, Chemical Biology Core Facility, EMBL, Heidelberg, Germany.

  • Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo. Overview image: Phase Gradient Contrast with fluorescence acquisition. High-resolution images: Airyscan 2 Multiplex.

    Lateral line primordium migration and deposition of immature neuromasts in a Zebrafish embryo. Overview image: Phase Gradient Contrast with fluorescence acquisition. High-resolution images: Airyscan 2 Multiplex. Sample courtesy of J. Hartmann and D. Gilmour, EMBL, Heidelberg, Germany.

  • Rat cortical primary neuron culture. Deconvolved images using GPU-based deconvolution.

    Rat cortical primary neuron culture. Deconvolved images using GPU-based deconvolution. Sample courtesy of H. Braun, LSM Bioanalytik GmbH, Magdeburg, Germany.

  • Expansion microscopy in mouse brain. Left: whole brain; top right: axon bundles, bottom right: pyramidal cells.

    Expansion microscopy in mouse brain. Left: whole brain; top right: axon bundles, bottom right: pyramidal cells. Sample courtesy of S. Asano, Boyden lab, MIT, Cambridge, USA.

  • Growth of cultured cells imaged over 72 hours using phase gradient contrast. To quantify proliferation, cell region was detected using machine learning. The growth curve shows relative cell coverage over time

    Growth of cultured cells imaged over 72 hours using phase gradient contrast. To quantify proliferation, cell region was detected using machine learning. The growth curve shows relative cell coverage over time. Sample and assay courtesy of P. Denner, Core Research Facilities, German Center of Neurodegenerative Diseases (DZNE), Bonn, Germany.

  • Living cricket embryos mounted in low-melt agarose, expressing nuclear-localized GFP, multi-position experiment.

    Living cricket embryos mounted in low-melt agarose, expressing nuclear-localized GFP, multi-position experiment. Sample courtesy of S. Donoughe, Biological Labs, Harvard University, Cambridge, USA.

  • Starlet sea anemone (Nematostella vectensis) imaged with Airyscan 2 high sensitivity mode.

    Starlet sea anemone (Nematostella vectensis) imaged with Airyscan 2 high sensitivity mode. Sample courtesy of A. Stokkermans, Ikmi Group, EMBL, Heidelberg, Germany.

  • GFP HEK (Human Embryonic Kidney) cells

    Imaged through a 1 mm plastic bottom; images taken every 5 minutes for a total of 5 days

    Sample courtesy of Sanofi-Aventis Deutschland GmbH; R&D IDD / in vitro Biology, Frankfurt, Germany
  • Porcine kidney cell division

    100 z stacks over 29 minutes, imaged with improved SNR and resolution using LSM Plus

  • Top view of a young starlet sea anemone

    Maximum intensity projection of 69 z planes imaged with Airyscan 2 Multiplex

    Sample courtesy of A. Stokkermans, Ikmi Group, EMBL, ­Heidelberg, Germany
  • Drosophila embryos – Guided Acquisition

    Automatic identification and imaging of a subset from a group of fixed embryos

    Sample courtesy of Dr. G. Wolfstetter, University of Gothenburg, Germany

Downloads

    • 3D Imaging Systems

      Your Guide to the Widest Selection of Optical Sectioning, Electron Microscopy and X-ray Microscopy Techniques.

      Pages: 68
      File size: 5 MB
    • ZEISS Celldiscoverer 7

      Your Automated Platform for Live Cell Imaging

      Pages: 60
      File size: 13 MB
    • Automation in Microscopy.

      WILEY Special edition of Imaging & Microscopy – Collection of 4 white papers (Wolff, Pepperkok, Donoughe, Gelman)

      Pages: 20
      File size: 1 MB
    • Automating Cellular Imaging with ZEISS Celldiscoverer 7

      Technology Note

      Pages: 9
      File size: 2 MB
    • Plastic Labware for Optimal Results in Modern Life Science Microscopy

      Using ZEISS Axio Observer and ZEISS Celldiscoverer 7

      Pages: 10
      File size: 4 MB
    • ZEISS LSM 9 Family with Airyscan 2

      Multiplex Mode for Fast and Gentle ConfocalSuperresolution in Large Volumes

      Pages: 11
      File size: 3 MB
    • ZEISS Celldiscoverer 7

      Analysis of three-dimensional cell culture using fast and sensitive widefield microscopy

      Pages: 6
      File size: 1 MB
    • ZEISS Celldiscoverer 7

      High quality LSM imaging with autocorrection objectives using plastic labware.

      Pages: 8
      File size: 2 MB
    • ZEISS Celldiscoverer 7

      Instruction Manual (English, Polish)

      Pages: 90
      File size: 4 MB
    • ZEISS Celldiscoverer 7

      Instruction Manual (English + German)

      Pages: 178
      File size: 9 MB

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