Chemically Cleared Brain and Thick Tissues
Looking Deep Into Your Samples
With the rise of optical tissue-clearing techniques, voluminous specimens such as entire brains, organs or large model organisms can be imaged with cellular to subcellular resolution without the need for traditional, physical sectioning. This development has been critical for researchers investigating anatomy, vasculature, neural networks or connectomics.
Optical clearing methods, together with computing power, data storage capacity, intelligent rendering and analysis algorithms, and with the right microscopy technology, enable efficient analysis of these large samples.
Balance Large Sample Size, High Resolution Imaging and Fast Acquisition Speed with Light Sheet Microscopy
Light sheet fluorescence microscopy (LSFM) uses a unique illumination method to excite and collect entire planes of data at one time. With cleared samples, this means collecting amazingly high-resolution data throughout a large sample at extremely high speeds. ZEISS Lightsheet 7 equips you for fast imaging of chemically cleared brain, organs or large tissue sections.
Human brain organoid with ECi clearing
to study neuronal morphology
Imaged with light sheet microscopy. Sample courtesy of D. Reumann and J. Knoblich, Institute of Molecular Biotechnology (IMBA) of the Austrian Academy of Sciences, Austria
Mapping interneurons and Purkinje cells
from an entire mouse brain cleared using the CLARITY protocol.
Imaged using light sheet microscopy. Sample courtesy of E. Diel and D. Richardson, Harvard University, USA
Cleared in ethyl cinnamate to study vertebrate limb regeneration
Sample courtesy of W. Masselink, Research Institute of Molecular Pathology, IMP.
Image courtesy of P. Pasierbek, K. Aumayr, IMP BioOptics, Vienna, Austria
Achieve Incredible Resolution and Structural Detail in Cleared Samples with Confocal Microscopy
Confocal microscopy reveals minute, subcellular details and high-resolution structural information from deep within chemically cleared brains, organs or other tissues. The ZEISS LSM 9 confocal family with Airyscan is equipped with super-resolution capabilities and multiplex mode for enhanced acquisition speeds to quickly image the finest structures.
Large Overview Imaging with Cellular Resolution
Zoom microscopy with chemically cleared brain or large tissue samples enables cellular resolution within very large fields of view. The ZEISS Axio Zoom.V16 zoom microscope can provide you with a large, overview image and then allow you to zoom in and observe single cells within cleared tissues. Equip Axio Zoom.V16 with ZEISS Apotome 3 to add optical sectioning and enhanced structural information.
* The images shown on this page represent research content. ZEISS explicitly excludes the possibility of making a diagnosis or recommending treatment for possibly affected patients on the basis of the information generated with an Axioscan 7 slide scanner.