![マルチビームArray Tomographyを用いたVolume EM マルチビームArray Tomographyを用いたVolume EM]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.100.56.file/multibeam-array-tomography.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.360.203.file/multibeam-array-tomography.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.768.432.file/multibeam-array-tomography.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.1024.576.file/multibeam-array-tomography.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.1280.720.file/multibeam-array-tomography.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original.image_file.1440.810.file/multibeam-array-tomography.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography.jpg/_jcr_content/renditions/original./multibeam-array-tomography.jpg"})
Volume EM手法
マルチビームArray Tomography
高スループットで大容量の微細構造データを処理
ワークフローの略図
![マルチビームArray Tomographyの試料調製 マルチビームArray Tomographyの試料調製]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.100.100.file/multibeam-array-tomography-sample-preparation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.360.360.file/multibeam-array-tomography-sample-preparation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.768.768.file/multibeam-array-tomography-sample-preparation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original.image_file.1024.1024.file/multibeam-array-tomography-sample-preparation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original./multibeam-array-tomography-sample-preparation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original./multibeam-array-tomography-sample-preparation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-sample-preparation.png/_jcr_content/renditions/original./multibeam-array-tomography-sample-preparation.png"})
1
樹脂包埋試料を切削し、切片を並べます。各切片の厚さは通常30~70 nmで、切削順に切片を試料キャリアに貼り付けます。
![マルチビームArray Tomographyによる画像取得 マルチビームArray Tomographyによる画像取得]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.100.100.file/multibeam-array-tomography-image-acquisition.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.360.360.file/multibeam-array-tomography-image-acquisition.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.768.768.file/multibeam-array-tomography-image-acquisition.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original.image_file.1024.1024.file/multibeam-array-tomography-image-acquisition.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original./multibeam-array-tomography-image-acquisition.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original./multibeam-array-tomography-image-acquisition.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multibeam-array-tomography-image-acquisition.png/_jcr_content/renditions/original./multibeam-array-tomography-image-acquisition.png"})
2
連続する各切片は、マルチビーム走査電子顕微鏡(ZEISS MultiSEM)でそれぞれイメージングされます。
![セグメンテーションの処理 セグメンテーションの処理]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-processing-segmentation.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-processing-segmentation.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-processing-segmentation.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-processing-segmentation.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-processing-segmentation.png/_jcr_content/renditions/original./volume-em-processing-segmentation.png"})
3
取得した電子顕微鏡画像が処理され、デジタルに調整されて3Dデータセットになります。細胞コンパートメントを特定し、セグメント化することができます。
![3Dビジュアライゼーション解析 3Dビジュアライゼーション解析]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.100.100.file/volume-em-3d-visualization-analysis.png","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.360.360.file/volume-em-3d-visualization-analysis.png","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.768.768.file/volume-em-3d-visualization-analysis.png","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original.image_file.1024.1024.file/volume-em-3d-visualization-analysis.png","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/array-tomography/volume-em-3d-visualization-analysis.png/_jcr_content/renditions/original./volume-em-3d-visualization-analysis.png"})
4
セグメント化された3Dデータセットは、視覚化および調査を行い、統計的に分析することができます。
アプリケーション例
脳組織の神経ネットワークを大きなスケールで理解する
![30 nm厚の脳切片 高コントラスト染色プロトコルによって作製し、ATUMtome(切片をテープに収集するウルトラミクロトーム)で切削した30 nm厚の脳切片から、ピクセルサイズ4 nm、6.5分で取得した画像を合成した1平方ミリメートルのモザイク画像。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.100.100.file/multi-beam-mouse-brain.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.360.360.file/multi-beam-mouse-brain.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.768.768.file/multi-beam-mouse-brain.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain.jpg"})
![30 nm厚の脳切片 高コントラスト染色プロトコルによって作製し、ATUMtome(切片をテープに収集するウルトラミクロトーム)で切削した30 nm厚の脳切片から、ピクセルサイズ4 nm、6.5分で取得した画像を合成した1平方ミリメートルのモザイク画像。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.100.100.file/maginfication_multi-beam-mouse-brain.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.360.360.file/maginfication_multi-beam-mouse-brain.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.768.768.file/maginfication_multi-beam-mouse-brain.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/maginfication_multi-beam-mouse-brain.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/maginfication_multi-beam-mouse-brain.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/maginfication_multi-beam-mouse-brain.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain.jpg/_jcr_content/renditions/original.image_file.1920.1920.file/maginfication_multi-beam-mouse-brain.jpg"})
高コントラスト染色プロトコルによって作製し、ATUMtome(切片をテープに収集するウルトラミクロトーム)で切削した30 nm厚の脳切片から、ピクセルサイズ4 nm、6.5分で取得した画像を合成した1平方ミリメートルのモザイク画像。
![個別の六角形のマルチビーム視野(mFoV) 既存のデータから抽出された7つの個別の六角形のマルチビーム視野(mFoV)をまとめた代表的なデータ]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.100.100.file/multi-beam-mouse-brain-mfov.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.360.360.file/multi-beam-mouse-brain-mfov.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.768.768.file/multi-beam-mouse-brain-mfov.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-mfov.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-mfov.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-mfov.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-mfov.jpg"})
![個別の六角形のマルチビーム視野(mFoV) 既存のデータから抽出された7つの個別の六角形のマルチビーム視野(mFoV)をまとめた代表的なデータ]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.100.100.file/maginfication_multi-beam-mouse-brain-mfov.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.360.360.file/maginfication_multi-beam-mouse-brain-mfov.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.768.768.file/maginfication_multi-beam-mouse-brain-mfov.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/maginfication_multi-beam-mouse-brain-mfov.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/maginfication_multi-beam-mouse-brain-mfov.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/maginfication_multi-beam-mouse-brain-mfov.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-mfov.jpg/_jcr_content/renditions/original.image_file.1920.1920.file/maginfication_multi-beam-mouse-brain-mfov.jpg"})
既存のデータから抽出された7つの個別の六角形のマルチビーム視野(mFoV)をまとめた代表的なデータ
既存のデータから抽出された7つの個別の六角形のマルチビーム視野(mFoV)をまとめた代表的なデータ
![61枚の画像タイルからなる単一mFoV 61の電子ビームで並行して取得した61枚の画像タイルからなる単一mFoVの例。左から右に100 µm以上をカバーし、取得時間は通常わずか数秒です。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.100.100.file/multi-beam-mouse-brain-single-mfov.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.360.360.file/multi-beam-mouse-brain-single-mfov.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.768.768.file/multi-beam-mouse-brain-single-mfov.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-single-mfov.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-single-mfov.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-single-mfov.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original./multi-beam-mouse-brain-single-mfov.jpg"})
![61枚の画像タイルからなる単一mFoV 61の電子ビームで並行して取得した61枚の画像タイルからなる単一mFoVの例。左から右に100 µm以上をカバーし、取得時間は通常わずか数秒です。]({"xsmall":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.100.100.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","small":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.360.360.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","medium":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.768.768.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","large":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.1024.1024.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","xlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.1280.1280.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","xxlarge":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.1440.1440.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg","max":"https://www.zeiss.com/content/dam/rms/reference-master/applications/life-sciences/volume-em/multibeam-array-tomography/maginfication_multi-beam-mouse-brain-single-mfov.jpg/_jcr_content/renditions/original.image_file.1920.1920.file/maginfication_multi-beam-mouse-brain-single-mfov.jpg"})
61の電子ビームで並行して取得した61枚の画像タイルからなる単一mFoVの例。左から右に100 µm以上をカバーし、取得時間は通常わずか数秒です。