See beyond Structure with Fluorescence Correlation Spectroscopy

More and more often, your research needs require looking beyond the structure of fixed samples to examining live samples in as close to physiological conditions as possible. Laser scanning microscopes have evolved to provide the fast and gentle imaging conditions that are required for these measurements and are capable of capturing high resolution images of fast-moving living samples. While there is a wealth of information that can be gained from these structural datasets, did you know that laser scanning microscopes are also capable of looking beyond structure to the molecular and mechanistic details of the sample? This can be easily accomplished with ZEISS laser scanning microscopes through the spectroscopic techniques of fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS).

How to use FCS and FCCS in your research

With the FCS and FCCS acquisition modes for ZEISS ZEN software, you can easily record and analyze FCS and FCCS data through a fully integrated, user friendly platform you are already familiar with from your confocal imaging.

  • Switch quickly between confocal imaging and FCS acquisition.
  • Easily image with multiple positions, and use a variety of sample carriers.
  • Use built in curve fitting during the experiment or easily reanalyze the data after the experiment.

Use your existing detectors to measure:

  • Mechanisms of transport
  • Molecule mobility
  • Molecule density (concentration)
  • Molecule size
  • Reaction rate
  • Kinetics and more!

FCS and FCCS are available for LSM 980 and LSM 880 microscopes

For more information on how you can add FCS/FCCS to your LSM, please contact your local representative or email us at, or learn more on our website: