Unravel Mysteries at the Cellular Level

ZEISS Microscopy offers a broad range of advanced microscopy techniques tailored for cell biology research, supporting both
qualitative and quantitative analysis across multiple scales. Here are the key types available:

Light Microscopy

• Widefield Microscopy: Ideal for fast imaging of fluorescently labeled cells and tissues.
• Confocal Microscopy: Includes laser scanning confocal, providing optical sectioning and 3D reconstruction of cellular structures.
• Multiphoton Microscopy: Enables deep tissue imaging with reduced photodamage, useful for live tissue and intravital imaging.
  Super-resolution Microscopy:
  • Airyscan: Combines confocal imaging with enhanced resolution (lateral resolution down to 90 nm) and sensitivity.
  • Elyra: Structured Illumination Microscopy (SIM), PALM, and dSTORM techniques allow resolution beyond the diffraction limit

Electron Microscopy (EM):

• Scanning Electron Microscopy (SEM): Surface morphology imaging; ZEISS also offers solutions like:
  • Array Tomography and serial block-face imaging with ZEISS Volutome
  • 3D Volume Imaging with FIB-SEM for high-resolution 3D reconstructions of cells and organelles.

Correlative Microscopy

• CLEM (Correlative Light and Electron Microscopy): Integrates fluorescent light microscopy with EM to link functional data (live cell or fluorescence) with ultrastructural context.

Digital and AI-Enhanced Imaging

• AI-driven segmentation and analysis using ZEN software and ZEISS arivis for 3D/4D image visualization and quantification.

The ZEISS Celldiscoverer 7 and ZEISS LSM 980 and 990 with Airyscan 2 are optimized for live-cell imaging. They integrate incubation systems with precise control of temperature, CO₂/O₂ levels, and humidity, ensuring physiological conditions over long durations. The LSM 980 also offers gentle imaging with high sensitivity, making it ideal for time-lapse experiments with minimal phototoxicity.T

The ZEISS Lattice Lightsheet is ideal for long-term imaging of living cells due to its ability to provide high-resolution, three-dimensional images with minimal phototoxicity and photobleaching. This advanced imaging system uses lattice light-sheet illumination, which allows for gentle and continuous observation of cellular processes over extended periods, preserving cell viability and function while capturing dynamic events with exceptional clarity.

The Zeiss LSM 980 & 990 with Airyscan is ideal for long-term imaging of living cells because it utilizes Airyscan technology to achieve super-resolution imaging with enhanced sensitivity and reduced phototoxicity. This allows researchers to capture detailed, high-quality images of cellular processes over extended periods while maintaining cell health and viability. Additionally, the system's advanced environmental control features ensure optimal conditions for live-cell imaging, making it well-suited for prolonged studies of dynamic cellular events.

ZEISS provides multiple super-resolution modalities:

• Airyscan (on LSM 990/LSM 910) – Improves resolution down to ~90 nm laterally while enhancing signal-to-noise ratio and is particularly gentle on live samples.
• Lattice SIM Family – Offers Structured Illumination Microscopy (Lattice SIM²), PALM, and dSTORM, achieving resolutions down to 20–40 nm. These systems enable detailed imaging of organelles, cytoskeletal elements, and protein complexes with high temporal and spatial resolution.

Yes. ZEISS offers several systems optimized for imaging 3D cell cultures:

• ZEISS LSM 990 with Airyscan 2: Ideal for high-resolution 3D imaging with optical sectioning and improved depth penetration.
• ZEISS Lightsheet 7: Specialized for imaging cleared or live 3D specimens (e.g., organoids, embryos) with minimal phototoxicity.
• Celldiscoverer 7: Allows automated imaging of spheroids in multi-well platesunder physiological conditions.
• ZEISS Lightfield 4D: Acquire large volumes with a single image snap and speed up your screening experiments.

Image analysis tools (ZEISS arivis Pro, ZEN 3D) support full workflows from acquisition to 3D rendering and analysis.

Yes, ZEISS provides powerful AI-based tools for image segmentation, classification, and quantitative analysis, specifically designed to enhance cell biology workflows, inlcuding:

• ZEISS arivis Pro: A scalable 3D/4D image analysis software with deep learning plugins that support large-volume datasets, such as those generated from confocal, Airyscan, light sheet, or EM imaging. It allows segmentation of entire cell populations, tracking over time, and visualization of dynamic processes in high-dimensional data.