Introduction To 3D Volume Acquisition Using Atlas On Your ZEISS FIB-SEM

Atlas 3D is ZEISS’ acquisition platform that extends the capability of your ZEISS Crossbeam FIB-SEM tool. Automated 3D cross-section image acquisition is performed within a comprehensive multi-scale correlative environment. In this workshop, the workflow of Atlas 3D will be covered: navigation and correlation of image from various sources, setup and optimization of SEM acquisition, FIB milling and drift correction parameters for 3D data acquisition, and alignment of the resulting 3D image data stack.

This is a 3-hour virtual workshop that will be streamed live from the  ZEISS Microscopy Customer Center (ZMCC) in Dublin, CA. Attendees will be able to view the operation of the instrument via screen-sharing and multiple web cameras and observe the operation of the tool as our FIB-SEM expert covers the topics detailed below.

This workshop is an introduction to 3D volume data acquisition with a focus on using Atlas 3D software on a ZEISS FIB-SEM tool. It is highly recommended that attendees have a basic understanding of scanning electron microscopy (SEM) and experience with Ga+ focused ion beam (FIB) milling. In addition, it is recommended that attendees secure time on their own instruments after the workshop session to practice what was shown using their own samples.

Who should attend?

• New users of Atlas 3D.
• Infrequent Atlas 3D users in need of a refresh on 3D setup and parameter optimization.
  
• Lab managers, engineers or technicians looking to improve their own skill level.
  

Learning objectives after completion of this workshop, attendees should:

• Gain an improved understanding of the Atlas 3D software and be able to efficiently navigate to the menus and settings used for 3D data acquisition.
  
• Improve their understanding of the 3D acquisition workflow using Atlas 3D on a ZEISS FIB-SEM instrument.
  
• Benefit from reproducible results by improving imaging and milling parameter selections that optimize the quality of the 3D data.
  
• Share results with a wider audience by exporting acquired images and data.

This is a paid workshop.​
Spots are limited.

​Access to a SEM after each day is strongly recommended to apply lessons learned and share results with applications team for further support.​

Preview the topics covered below and register for the workshop here.