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Workshop

Conquer the Confocal

A ZMCC SkillBuilder Workshop

Register for the workshop

Workshop Overview & Agenda

This workshop will cover methods and supporting theory to enable attendees to become more comfortable and competent operating their ZEISS LSM 900s and 980s. Prior LSM imaging background will be helpful but not required. It is recommended that attendees secure time on their instruments after the workshop sessions to apply the shared methods on their own challenging samples. Attendees are strongly encouraged to share their images from individual sessions and interact with our Applications team through independent email threads for further optimization.

This is a two-day in-person paid workshop located at the ZEISS Microscopy Customer Center (ZMCC) in Dublin, CA. The workshop will consist of comprehensive sessions of hands-on instrument practice as well as engaging classroom lectures and discussions. With small group sizes of <5 attendees/trainer, you will get valuable, individualized access to the most cutting-edge microscopy solutions ZEISS offers.

Who should attend?

  • Existing users of ZEISS LSMs (700/800/880/900/980)
  • Infrequent LSM users looking for a refresher on basic and advanced LSM techniques
  • Lab managers or technicians looking to improve their own skill level to better support and/or train other users of the instrument

Learning objectives after completion of this workshop, attendees should:

  • Possess an improved ability to select imaging parameters to yield high-quality images of their samples
  • Have greater confidence with complex imaging setups, optimizing imaging parameters, troubleshooting on LSMs and analyzing acquired datasets
  • Be connected with the North American Applications team who can support them as they work towards acquiring the best data possible

Workshop Package Inclusions:

  • World-class training and certification at the end of workshop
  • Hotel accommodation within walking distance
  • Lunches and refreshments during the workshop

Payment Deadline:
This paid workshop costs $2,400 per attendee.  To guarantee your room and hotel coverage, please make sure to submit your payment by November 7. To secure your seat in the workshop, the absolute payment deadline is November 22.

Preview the topics covered below and register for the workshop here. 

 

Day 1

Session A: Introduction to Confocal

  • What is a pinhole and how does optical sectioning work
  • Why pixel size matters
  • LSM Lightpaths
  • Varying Imaging Conditions
    • How to take good images (dynamic range; axial resolution and sampling; etc)
    • Speed v. Spectral Separation

Session B: Image Analysis using ZEISS arivis

  • ZEISS arivis basics – uploading, exporting, display settings
  • Analysis pipeline foundations
  • Introduction to ZEISS arivis machine learning

Day 2

Session C: Spectral Imaging on LSM 980

  • LSM 980 beam path and detector overview (PMTs; Spectral GaAsP; NIR detectors)
  • Far red sensitivity on NIR
  • Linear Unmixing
  • Spectral imaging in Lambda Mode
  • Online Fingerprinting

Session D: Airyscan Imaging

  • LSM 900
    • Basics of the Airyscan detector
    • AS detector alignment
    • Superresolution and multiplex modes on LSM 900
  • LSM 980
    • Multiplex modes on LSM 980 (4Y and 8Y)
    • AS detector alignment
    • Imaging with live cells on the Airyscan

Rebecca Duffy, Ph.D.

Applications Manager, Life Sciences

Dr. Duffy spent the beginning of her career training as a tissue engineer and applying advanced 3D imaging techniques to quantify engineered organs-on-a-chip before becoming an applications specialist for ZEISS. She attended Virginia Commonwealth University to earn her B.S. in Biomedical Engineering, and she pursued her graduate studies at Carnegie Mellon University in in Adam Feinberg’s lab where her research focused on engineering contractile skeletal and cardiac muscle microtissues. She continued her in vitro tissue engineering work through a collaborative post-doc in Boehringer Ingelheim’s SHINE program where she worked in Linda Griffith’s lab at MIT. For this project she focused on building 3D intestinal fibrosis models to measure Collagen Type I assembly from patient-derived cells in 3D microenvironments using laser scanning confocal and multi-photon imaging. From there she moved on to work as a scientist at United Therapeutics, working to build technology to 3D print human lungs for transplant patients. During this time, she established key cellular attachment, proliferation, and migration assays used to assess in-house developed bioinks, and she used a suite of ZEISS microscopes to assess cellularization of 3D printed scaffolds (Celldiscoverer 7, LSM 880, LSM 980, Lightsheet 7).

Elise Shumsky

Head of Product Application Sales Specialist Team in North America, ZEISS Research Microscopy Solutions

Joining ZEISS more than 40 years ago as a Scientific Programmer, Elise began her career programming our Light and Electron Microscopes. She holds a BS in both Chemistry and Mathematics from Binghamton University and obtained her master’s in computer science from Pace University. After 3 years of programming, she transitioned into the Product Management group where she spent the next 10 years as the Product Manager for Image Analysis. She then joined the field Sales team as the Field Product Specialist for Confocal Microscopy at ZEISS, in the Mid-Atlantic Region, before becoming the Head of the NA Product Application Sales Specialist group.

Register for the workshop

Registration is now closed.

Thank you for your interest in the November session of this workshop. Registration is now closed. If you initiated a registration, please note that to guarantee your seat in the workshop, the payment deadline is November 22, 2023.

Workshop Location

ZEISS Microscopy Customer Center, Bay Area

ZMCC, Bay Area 5300 Central Pkwy 94568 Dublin, CA USA
Website
800 233-2343
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