Workshop

Regeneron ZEISS Day

Learn How Microscopy Can Accelerate Your Research

Tue, 10 September 2024

A 1-day workshop @ Regeneron Pharmaceuticals

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How to Choose the Right Microscope for Your Experiment

Presented by: Kalliopi Arkoudi, Ph.D, Applications Development Scientist

This presentation will focus on how the different microscopy technologies can help you achieve your experimental goals. Topics to be covered include:

Brightfield and Fluorescence microscopy
2D imaging & 3D sectioning
Imaging workflows
Automation
LSM 900 & 980; New Features & Capabilities on Your Campus

Presented by: Antonio Tedim Pedrosa, Ph.D., Product Application Sales Specialist

Overview of the new LSM 900 & 980 microscopes, including the new features and capabilites available on campus, and specific use cases highlighting their optimal use.
Optimizing Imaging Parameters

Presented by: Christian Hellriegel, Ph.D., Product Application Specialist

Optimizing imaging parameters for both confocal and widefield microscopes:

Practical tips: coverslip choice and Nyquist resolution.
Objective choices: live cell imaging, high resolution imaging, fixed endpoint imaging
How to set up confocal experiments properly: avoiding bleedthrough, gain, laser power, average, etc.
ZEISS Q&A

Come prepared with your questions on microscopy. Lunch will be provided!
Lattice Lightsheet 7 – Track Cellular & Organelle Dynamics from minutes to hours

Presenters: William Briley, Ph.D., Applications Scientist

Introduction to the Lattice Lightsheet-7, including a live demo on the instrument. We will explore why the LLS7 is capable of performing volumetric imaging faster and gentler than any other widefield, confocal, or spinning disk microscope, often by several orders of magnitude. After discussing the technology, there will be a live demonstration of the LLS7, illustrating how the discussed benefits apply practically to imaging live cells and organisms, enabling 4D imaging that is both highly time-resolved and spatially-resolved far beyond what is possible with other microscope systems.
Lattice SIM – Receptor Clustering & Endosomal Trafficking at Super Resolution

Presenters: William Briley, Ph.D., Applications Scientist

Introduction to the Zeiss Lattice SIM family of instruments, the newest in the Zeiss light microscopy portfolio, including a live demo on one of the instruments performing gentle, live cell, super-resolution imaging. After introducing the technology, we will use the Lattice SIM-3 to image rapidly moving endosomes in live cells with very high resolution and speed. During the demonstration we will also illustrate how the inclusion of automation and AI have made these instruments easy to use, even for those with little to know microscopy background, and how Lattice SIM imaging can simplify and expedite particle tracking and other analyses.

William Briley, Ph.D. Product Applications Specialist

William Briley is responsible for ZEISS’ advanced 3D imaging solutions at the Zeiss Microscopy Customer Center (ZMCC), including the Lattice SIM family, Lattice Lightsheet 7, and Lightsheet 7. He has been with ZEISS for 4 years and has 18 years of microscopy experience across a spectrum of biological and nonbiological applications. Will holds a B.S. and M.S. in Chemical Engineering, and a Ph.D. in Interdisciplinary Biological Sciences from Northwestern University.

Kalliopi Arkoudi, Ph.D. Applications Development Scientist for Pharma & Biotech

Kalliopi Arkoudi brings a robust expertise in developing advanced microscopy applications and workflows within the realm of life science research. With a focus on advanced fluorescence microscopy, spectral multiplexing, super-resolution imaging, and molecular dynamics, she has consistently pushed the boundaries of imaging capabilities. Kalliopi earned her doctorate in Inflammation and Regenerative Medicine research from King's College London in 2020, where she developed an innovative NLO system for studying protein-protein interactions in vivo.

Antonio Tedim Pedrosa, Ph.D. Product Application Sales Specialist

Antonio Pedrosa is a Product Application Sales Specialist for the northeast region including Regeneron. He is responsible for providing technical sales support for laser-based life science systems and high-end wide field microscopes. Antonio joined ZEISS after completing his post-doctoral training at NHLBI/NIH, where he used advanced microscopy techniques to study the role of Myo10 in cancer cell division. During his training, he worked with several microscopes, including LSM 880 Airyscan, and was involved in beta testing the ZEISS Lattice Lightsheet microscope.

Christian Hellriegel, Ph.D. Product Application Sales Specialist

Christian Hellriegel is an Automated Product Application Sales Specialist at ZEISS, specializing in Axioscan and Celldiscoverer. His journey with ZEISS began in 2011, and he has since served as an embedded specialist at Harvard's HCBI imaging facility. Christian holds a doctorate from Ludwig-Maximilians-Universität, Munich, and has done postdoctoral work at UC Irvine. He has a diverse expertise in advanced microscopy techniques including super resolution and localization microscopy.